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S Jablonska

Publications and source records attributed to S Jablonska.

At least 145 records · Page 8Linked to original sources
PubMed1989

Apoptosis in Bowen's disease. An ultrastructural study.

We performed ultrastructural studies of apoptosis (previously referred to as "malignant dyskeratosis") in a case of genital Bowen's carcinoma in which human papillomavirus (HPV) type 33 genome was identified and in two cases of cutaneous Bowen's disease with no detectable viral DNA; herein we present the sequential stages in the development of apoptotic bodies. The apoptotic process in the HPV-containing genital Bowen's disease was similar to that in the cutaneous lesions with no detectable HPV. The presence of a large number of apoptotic bodies in Bowen's disease may be responsible for the slow progression and noninvasive growth of this carcinoma in situ.

Adult↗
PubMed1989

Characteristic nucleolar-reticular immunofluorescence staining pattern on touch prints of hamster liver: a marker for Scl-70 antibody.

Sera from 48 patients with systemic sclerosis (SSc) and from 62 patients with other connective tissue diseases were studied for nuclear staining patterns by indirect immunofluorescence (IIF) using touch prints of hamster liver, HEp-2 and cryostat sections of monkey esophagus as substrates. Immunodiffusion studies performed in all cases disclosed Scl-70 antibody in 36 SSc patients. The IIF pattern of Scl-70 antibody is characterized by diffuse granular and nucleolar staining on HEp-2, a combined nucleolar and diffuse reticular staining on imprints of hamster liver and large speckled (nucleolar) fluorescence on cryostat sections of monkey esophagus. Touch prints of hamster liver revealed a partially nucleolar fluorescence as a dominant sign of the combined staining. Hamster liver substrate which is easily available might replace the expensive HEp-2 cells for detecting Scl-70 antibody.

Animals↗
PubMed1989

Two subpopulations of patients with early and late onset of psoriasis differ regarding alpha 1-proteinase inhibitor activity.

Two subpopulations of patients with psoriasis were identified when activity of alpha 1-proteinase inhibitor was analysed according to the patient's age at onset of the disease. Patients with early onset had more active lesions, high incidence of familial psoriasis, and reduced alpha 1-proteinase inhibitor during flare similar to that activity in remission. In contrast, alpha 1-proteinase activity increased significantly during flare in patients with late onset (above 21 yrs of age) in response to the inflammatory process in the psoriatic skin.

Adolescent↗
PubMed1989

[The Scl-70 antibody and its clinical significance].

In the review of the literature on the Scl-70 antigen, methods of detection of the Scl-70 antibody, the correlation between its presence and the clinical, genetic and immunological parameters are presented. It is emphasized that the Scl-70 antibody which is regarded as a marker of diffuse scleroderma, may have a prognostic significance. In indirect immunofluorescence on HEp-2 cells the Scl-70 antibody gives a characteristic pattern described as diffuse fine speckles with dotted nucleolus. A somewhat different but distinct staining pattern on hamster liver described by the authors was found to be characteristic of that antibody. The fluorescence associated with Scl-70 antibody is designated as nucleolar-reticular mixed staining with nucleolus predominating. It is suggested that hamster liver substrate which is easily available may replace the expensive HEp-2 cells for detecting Scl-70 antibody.

Antibodies, Antinuclear↗
PubMed1989

[The PM-Scl (polymyositis-scleroderma) autoantibody and its nucleolar fluorescence pattern].

By indirect immunofluorescence studies of antinuclear antibodies on hamster liver imprints as a substrate performed to determine the nuclear staining pattern of sera from patients with connective tissue diseases 10 sera showed a distinct homogeneous nucleolar staining pattern associated with weaker speckled or homogeneous nucleoplasmic fluorescence. In all 10 cases the antibodies revealed the PM-Scl specificity in immunodiffusion. Clinically 9 patients had an acrosclerosis, in 56% overlapped with symptoms of polymyositis. Only one patient had a diffuse scleroderma. The homogeneous nucleolar immunofluorescence pattern of PM-Scl should be distinguished from mixed nucleolar and diffuse reticular nucleoplasmic pattern of Scl-70.

Antibodies, Antinuclear↗
PubMed1988

Human papillomavirus 8-L1 immune serum: a new diagnostic possibility for Epidermodysplasia verruciformis-specific HPVs.

Human papillomavirus (HPV) 8 induces flat macular skin lesions with a high risk of malignant conversion. A 600-bp fragment from the center of the major structural protein gene L1 was cloned into pEX2 to produce a beta-galactosidase-L1 fusion protein. The expressed part of L1 is masked in intact virions and not detected by sera of infected patients. Immunization of guinea pigs with purified fusion protein raised high-titer antisera, which reacted with capsid proteins of HPV8 and closely related viruses in Western blot and indirect immunofluorescence tests. Structural proteins of HPV1, 2, and 3 were not detected, indicating specificity for the subgroup of ev-specific HPVs. The sera present themselves as convenient diagnostic reagents for the demonstration of infections with potentially oncogenic HPVs using routine immunofluorescence procedures.

Antibody Specificity↗
PubMed1988

Role of cell-mediated immunity in spontaneous regression of plane warts.

Human papillomavirus-induced plane warts most often occur in the second decade of age. Afterward, they either spontaneously regress or are eradicated in the course of various treatments. As proved by in vivo and in vitro tests as well as clinical observations, they most often affect and persist longer in immunocompromised hosts. In this work it was confirmed that specific-ie, anti-HPV-directed, cell-mediated immunologic response plays a role in spontaneous regression of plane warts and that preservation of nonspecific immunity is prerequisite for spontaneous regression of plane warts.

Adolescent↗
PubMed1988

Etretinate in severe psoriasis of children.

Ten children, five with generalized pustular psoriasis and five with erythrodermic disease, were treated with etretinate and observed for up to seven years. In all those with pustular psoriasis, complete clearing was obtained in three weeks to four months, but in one child maintenance therapy had to be introduced twice, for one year each time. In those with erythrodermic psoriasis, the results were favorable only in two, and in two it was necessary to introduce Re-PUVA. Clinical side effects were slight, and laboratory examinations did not disclose any significant abnormalities. In one child who was treated intermittently for seven years, focal osteoporosis of one tibia was disclosed by radiography. No adverse effects on children's development were noticed.

Child↗
PubMed1988

Action of topically applied arachidonic acid on the skin of patients with psoriasis.

Concentrations of arachidonic acid ranging from 0.1% to 2% were applied under occlusive dressings to psoriatic plaques in 45 patients. Alleviation of the clinical symptoms of psoriasis including complete clearing in some cases was obtained with the use of 0.5% to 2% arachidonic acid applied under occlusion every 24 to 48 hours five to seven times. Histologic examination showed polymorphonuclear leukocytes penetrating into the stratum corneum and formation of microabscesses or wide-spread accumulations of polymorphonuclear leukocytes in the stratum corneum, with its eventual destruction. The parakeratotic horny layer became detached; this was followed by restoration of the granular layer and an apparently normal stratum corneum. While arachidonic acid metabolites can be proinflammatory and proproliferative, they may also be important in the healing process for psoriasis.

Administration, Topical↗
PubMed1988

Scl-70 antigen stability and its effect on antibody detection in scleroderma.

We examined 38 patients with scleroderma, 10 with systemic lupus and 10 normal subjects for Scl-70 antibodies by the gel precipitation and by the immunoblot methods. Increased incidence of Scl-70 antibodies in scleroderma were found by the immunoblot method (55%) compared to the gel precipitation methods (40 or 42% depending on the test kits used). Immunoblot analysis of the antigen prepared with 4.0 M NaCl extraction of rabbit thymus acetone powder revealed antigen to be 100, 86, 80 and 70 kDa. However, mainly a single band of 70 kDa was obtained upon extraction of rabbit thymus with 0.3 M NaCl and of calf thymus antigen. Our data support the suggestion that different molecular species of the Scl-70 antigen have variable binding affinities to nuclear DNA. We suggest that the presence of various molecular forms of the antigen may be a result of this differential binding affinity to DNA and the partial proteolytic digestion of 100 kDa from the antigen as reported by others.

Adult↗