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Biomedical subjects

S J Southern

Publications and source records attributed to S J Southern.

17 recordsLinked to original sources

Reconstruction of the high-risk chest wall with endoscopically assisted latissimus dorsi harvest and expander placement.

Certain patients requesting breast reconstruction may be described as having a high-risk chest wall with regard to implant loss and well-documented high complication rates. Such patients have a combination of one or more of the following: previous chest wall radiotherapy, heavy smoking, and thin, tethered chest wall flaps. If autologous transfer is not appropriate for such patients then reconstruction may be difficult. In this specific patient group the assistance of endoscopy has been used to raise the latissimus dorsi muscle to cover an expander placed within an endoscopically created chest wall pocket. The first 12 consecutive cases using this technique are discussed, showing an expander loss rate of 8% for the primary implant placement.

Breast Implantation↗

Endoscopic rectus harvest: a simplified sheath-saving technique.

The potential benefits of harvesting the rectus abdominis muscle endoscopically are great. To date no simple, reliable method has been developed for harvest based on the inferior epigastric artery and transferred to the living patient. We have devised a simplified endoscopically assisted method of harvest on a cadaveric model that adds speed and the advantage of a grossly intact sheath to other published methods. It has been used successfully in a clinical situation. Two-portal access and triplanar dissection are the key points in this method.

Adult↗

A skin model for wound closure tuition.

An easy to make skin model has been developed using commonly available materials. It allows simple wound closure to be practised safely on a medium which attempts to simulate the properties of soft tissue.

Dermatologic Surgical Procedures↗

A comparison of titration methods for live avian encephalomyelitis virus vaccines.

Factors associated with the indirect fluorescent antibody test used for the titration of avian encephalomyelitis virus (AEV) in chick embryo brain cell cultures were examined for their influence on virus replication. It was found that virus should be inoculated onto semi-confluent cell cultures and adsorbed for two hours at room temperature. The cells should then be examined for fluorescence after five days' incubation. Using these conditions, the cell culture assay was compared with the embryo and chick assays for its ability to estimate the virus content of live commercial AEV vaccines. In most cases titres obtained by the chick assay were slightly, but not significantly, higher than those obtained in the cell culture assay, although the reliability of the chick assay was, at times, questionable. In all cases titres obtained in the embryo assay were low. It is recommended that the cell culture assay be adopted as the method of choice for titrating AEV vaccines because it is rapid, reproducible, specific and greatly reduces the requirement for experimental animals.

Animals↗