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Biomedical subjects

S Inouye

Publications and source records attributed to S Inouye.

At least 523 records · Page 29Linked to original sources
PubMed1975

Bioconversion of ribostamycin (SF-733). II. Isolation and structure of 3-N-acetylribostamycin, a microbiologically inactive product of ribostamycin produced by Streptomyces ribosidificus.

The isolation and structure determination of 3-N-acetylribostamycin, a microbiologically inactive derivative, produced enzymatically from ribostamycin by Streptomyces ribosidificus is described. The location of the acetyl group was established by mass and NMR spectrometry of the new compound and its derivatives, and by optical rotation studies conducted on N-ethoxycarbonyl-2-deoxystreptamine. The latter compound was obtained by partial acid hydrolysis of tri-N-ethoxycarbonyl-N-acetylribostamycin.

Acetyltransferases↗
PubMed1975

Bioconversion of ribostamycin (SF-733). III. Formation, structure and synthesis of 3-N-carboxymethyl ribostamycin.

A new inactivated product of ribostamycin (SF-733), 3-N-carboxymethyl ribostamycin, was obtained from the broth of Streptomyces ribosidificus which was grown on a medium containing D-xylose. Detection and some biochemical mechanism of N-carboxymethylation were discussed, and structure of 3-N-carboxymethyl ribostamycin was proposed based on the chemical degradation and synthesis.

Anti-Bacterial Agents↗
PubMed1973

Nonspecific adsorption of proteins to microplates.

When a solution of purified adenovirus hexon proteins was diluted in polystyrene microplates without carrier proteins in the diluent, a higher rate of hexon adsorption to the microplates at higher dilutions took place. Addition of gelatin (0.01%), bovine serum albumin (0.01%), or calf serum (0.2%, vol/vol) to the diluent effectively prevented this adsorption. The adsorption to the microplates of adenovirions and measles virions was also prevented by the carrier proteins in the diluent. Certain additional features which distinguish the microtechnique of Takatsy from conventional macromethods are also discussed.

Adenoviridae↗
PubMed1972

Effect of a modified kaolin treatment on serum immunoglobulins.

After kaolin treatment of fetal rabbit serum, 7S antibody titers were reduced more than 19S titers. This reduction was less when the kaolin treatment was performed at pH 9.0 than when it was performed at pH 7.3. A modification of the kaolin treatment of sera for use in the hemagglutination-inhibiting antibody titration, in which the hemagglutination reaction is performed at a neutral pH, is recommended. The advantage of the modified method is that adsorption of immunoglobulins to kaolin is minimized when serum is treated at a lower dilution with pH 9.0 kaolin, followed by reduction of the pH of the supernatant fluid to neutrality with a "serum adjusting diluent." When the serum was diluted with physiological saline before kaolin treatment, a great decrease in serum immunoglobulin concentrations occurred. This decrease was found to be less in the modified kaolin treatment than in the conventional pH 7.3 kaolin treatment.

Adsorption↗