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S Inoue

Publications and source records attributed to S Inoue.

At least 1,351 records · Page 75Linked to original sources
PubMed1985

The basement-membrane-like matrix of the mouse EHS tumor: II. Immunohistochemical quantitation of six of its components.

The presence of six substances--laminin, type IV collagen, heparan sulfate proteoglycan, entactin, fibronectin, and the amyloid P component--was investigated immunohistochemically in the matrix of the Engelbreth-Holm-Swarm (EHS) mouse tumor after it had been fixed in formaldehyde (with or without a brief preliminary glutaraldehyde fixation), embedded in Lowicryl K4M, and sectioned for processing through the protein A-gold sequence. Enumeration of the number of gold particles per square micrometer of matrix sections demonstrated that the six substances were present in distinct amounts. The results for each substance were fairly consistent throughout the matrix in three experiments. Furthermore, the available evidence indicated that, with the exception of the amyloid P component, the substances were associated with the cord network of the tumor matrix. Finally, the use of a reconstituted basement membrane containing known amounts of laminin, type IV collagen, and heparan sulfate proteoglycan as a standard, led to the conclusion that, in the tumor matrix, the relative content of laminin to type IV collagen to the proteoglycan was in a ratio of 1:0.6:0.03, suggesting molar ratios of approximately 1:1:0.2, respectively.

Animals↗
PubMed1985

The basement-membrane-like matrix of the mouse EHS tumor: III. Immunodetection of the amyloid P component in basotubules.

Immunohistochemical methods were applied to the ultrastructural localization of the amyloid P component in the EHS tumor matrix. First, the preembedding approach was used by exposing frozen sections of tumor to antiserum against the mouse amyloid P component followed by the peroxidase-antiperoxidase sequence. Second, using the postembedding approach, Lowicryl K4M sections of the tumor were exposed to antiserum against the amyloid P component and subjected to the protein A-gold procedure. In both cases, the immunostaining was restricted to structures which appeared in longitudinal section as fairly straight rods and in cross section as 7- to 10-nm pentagonal or roughly circular profiles outlining a lumen with a central dot. Since these features are characteristic of basotubules, it is concluded that the basotubules of the tumor matrix possess the antigenicity of the amyloid P component and presumably contain this substance itself. Similar experiments carried out on the thick basement membrane known as Reichert's membrane demonstrated that its basotubules also possessed amyloid P-component antigenicity. It is likely, therefore, that the amyloid P component is a constituent of basotubules.

Amyloid↗
PubMed1985

Two natural killer-cell subpopulations distinguished by heat sensitivity.

We examined the effect of heat on natural killer-cell activity and found that two different natural killer-cell subpopulations can be distinguished by their heat sensitivity; one subpopulation loses natural killer-cell activity at 41 degrees C, and the other is not affected. In a single-cell assay, the ability of natural killer cells to conjugate to K 562 cells was not affected by incubation at 41 degrees C, but the killer activity of natural killer cells after conjugating to K 562 cells was reduced at 41 degrees C. Therefore it is likely that the difference in heat sensitivity between the two subpopulations is due to postbinding cytolytic events. Tetracaine, which influences cytolytic events, was used to examine whether or not the two natural killer-cell subpopulations can be distinguished by tetracaine sensitivity. However, it was found that tetracaine inhibits natural killer-cell activity equally for both of these natural killer-cell subpopulations.

Hot Temperature↗
PubMed1985

Cyclosporine and partial liver allotransplants in a simplified rat model.

We designed a simplified rat model to assess the effects of cyclosporine (CsA) on liver transplants. Auxiliary liver grafts consisting of 20 per cent of the whole liver were supplied with portal inflow and the bile was drained into the stomach. The cuff technique was used routinely for both end-to-end and end-to-side venous anastomoses. The host liver was totally deprived of portal inflow. Two inbred strains of rats, LEW and F344, were used for isograft (LEW----LEW) and allograft (F344----LEW). All survivors were killed at the end of the fourth week after transplantation. During four weeks, the isografts increased by 108 per cent in wet liver weight. Animals with allograft were treated with CsA given i.m., in a daily dose of 20 and 40 mg/kg for the first ten days post transplantation. The CsA-treated rats were free from parenchymal destruction of the allograft, as was characteristic of the allograft controls. The rats given CsA in the dose of 40 mg/kg showed a 173 per cent increase in the wet liver weight of the transplant, such being greater than allografts in rats on 20 mg/kg of CsA (100 per cent) and even than isografts. These results indicate that auxiliary partial liver grafts benefit from the use of CsA not only through suppression of allorejection but through a potential "hepatotrophic effect" of the agent.

Alanine Transaminase↗
PubMed1985

Hepatic triglyceride lipase is not an insulin-dependent enzyme in rats.

The aim of this study is to examine whether insulin regulates the activity of hepatic triglyceride lipase. The activities of both hepatic triglyceride lipase (H-TGL) and lipoprotein lipase (LPL) were measured in plasma after intravenous administration of heparin. Insulin-deficient diabetic rats had normal H-TGL activity. Insulin-treated normal rats had no different enzyme activities from those of controls. Ventromedial hypothalamic (VMH) lesioned rats that became obese and showed hyperinsulinemia, had also normal enzyme activities. From these experiments, it is concluded that H-TGL is not an insulin-dependent enzyme. As far as LPL is concerned, this enzyme was increased in both insulin-treated rats and rats with VMH lesions. This was consistent with the established fact that insulin has a stimulatory effect on adipose tissue LPL. The LPL activity in postheparin plasma was not decreased in insulin-deficient rats. It is speculated that a possible increase in muscle LPL compensated for the low adipose tissue LPL in diabetic rats.

Animals↗
PubMed1985

A case of verrucous carcinoma of the uterine cervix: clinical, light, and electron microscopic, and immunohistological observations.

A case of verrucous carcinoma of the uterine cervix in a 67-year-old patient is reported. On transmission electron microscopy, virus-like particles with a diameter of 45 to 55 nm were found in the nuclei of koilocytotic cells. However, human papilloma virus antigen could not be detected by immunohistochemistry, using the peroxidase-antiperoxidase technique.

Aged↗
PubMed1985

Rationale for bone marrow transplantation in the treatment of autoimmune diseases.

Transplantation of normal bone marrow from C3H/HeN nu/nu (H-2k) mice into young MRL/MP-lpr/lpr (MRL/l; H-2k) mice (less than 1.5 mo) prevented the development of autoimmune diseases and characteristic thymic abnormalities in the recipient mice. When female MRL/1 (greater than 2 mo) or male BXSB (H-2b) mice (9 mo) with autoimmune diseases and lymphadenopathy were lethally irradiated and then reconstituted with allogeneic bone marrow cells from young BALB/c nu/nu (H-2d) mice (less than 2 mo), the recipients survived for more than 3 mo after the bone marrow transplantation and showed no graft-versus-host reaction. Histopathological study revealed that lymphadenopathy disappeared and that all evidence of autoimmune disease either was prevented from developing or was completely corrected even after its development in such mice. All abnormal T-cell functions were restored to normal. The newly developed T cells were found to be tolerant of both bone marrow donor-type (BALB/c) and host-type (MRL/1 or BXSB) major histocompatibility complex (MHC) determinants. Therefore, T-cell dysfunction in autoimmune-prone mice can be associated with both the involutionary changes that occur in the thymus of the autoimmune-prone mice and also to abnormalities that reside in the stem cells. However, normal stem cells from BALB/c nu/nu donors can differentiate into normal functional T cells even in mice whose thymus had undergone considerable involution, as in the case of BXSB or MRL/1 mice in the present studies. These findings suggest that marrow transplantation may be a strategy ultimately to be considered as an approach to treatment of life-threatening autoimmune diseases in humans. T-cell dysfunction in autoimmune-prone mice previously attributed to involutionary changes that occur in the thymus of these mice may instead be attributed to abnormalities that basically reside in the stem cells of the autoimmune-prone mice.

Animals↗
PubMed1985

Complete amino acid sequence of cytochrome c from the honeybee, Apis mellifera, and evolutionary relationship of the honeybee to other insects on the basis of the amino acid sequence.

The complete amino acid sequence of cytochrome c purified from the honeybee, Apis mellifera was determined. Only one molecular species of cytochrome c was found in the honeybee throughout its metamorphic stages. On the basis of a comparison of the amino acid sequence of honeybee cytochrome c with those of cytochromes c from other insects, it seems that the bee has evolutionarily appeared earlier than would be expected from the morphological and fossil evidence. If the classical phylogenetic relationships of the honeybee are correct, the evolutionary rate of cytochrome c must have been more rapid in the honeybee than in other insects.

Amino Acid Sequence↗
PubMed1985

A micro-scale method for the conjugation of affinity-purified Fab' to beta-D-galactosidase from Escherichia coli.

A micro-scale method for the conjugation of affinity-purified Fab' to beta-D-galactosidase from Escherichia coli is described. Rabbit anti-human chorionic gonadotropin serum (0.2 ml) was digested with pepsin to convert IgG to F(ab')2 and applied to a column of human chorionic gonadotropin-Sepharose 4B, followed by elution at pH 2.5. The affinity-purified anti-human chorionic gonadotropin F(ab')2 was mixed with non-specific goat F(ab')2 (0.5 mg) as a carrier, reduced with 2-mercaptoethylamine to split F(ab')2 to Fab' and conjugated to beta-D-galactosidase using N,N'-o-phenylenedimaleimide. The affinity-purified rabbit anti-human chorionic gonadotropin Fab'-beta-D-galactosidase conjugate was separated from non-specific goat Fab'-beta-D-galactosidase conjugate and unconjugated beta-D-galactosidase by affinity chromatography on a column of goat (anti-rabbit IgG) IgG-Sepharose 4B using 4 M urea. The amount of the affinity-purified conjugate obtained was 56-69 micrograms. The detection limit of human chorionic gonadotropin by a sandwich enzyme immunoassay technique was improved 30-fold by using the affinity-purified conjugate as compared with that before affinity-purification. This method is applicable to the conjugation with alkaline phosphatase from calf intestine and probably also other enzymes which are stable in 4 M urea.

Animals↗
PubMed1985

One hundred hepatic resections. Indications and operative results.

The indications and operative results of hepatic resections were investigated in 100 consecutive patients over the past 20 years. There were 61 hepatocellular carcinoma, 13 hepatolithiasis, and 26 other miscellaneous diseases. An overall hospital mortality rate was 25%. It was 26, 0, and 35% in patients with hepatocellular carcinoma, hepatolithiasis, and other diseases, respectively. There was not any significant difference between survived and deceased cases in their preoperative laboratory data. The volume of operative blood loss in deceased cases was significantly larger than that in survived cases, so the influence of operative blood loss on morbidity and mortality was investigated. The incidences of postoperative bleeding, hepatic insufficiency, pulmonary insufficiency, and hospital death were significantly higher in patients whose operative blood loss exceeded 5000 ml. These results indicate that operative blood loss is one of the critical factors that decide the operative prognosis.

Adolescent↗
PubMed1985

Stiff skin syndrome.

Stiff skin syndrome was first documented by Esterly and McKusick. We saw a patient with this disease, as well as another who had a similar condition but a somewhat different histology. These two patients shared heritable stiff skin, restricted joint mobility, and absent mucopolysacchariduria. In stiff skin syndrome, which may have two variants, skin biopsy demonstrates large cells stained metachromatically by toluidine blue. These cells are shaped like dermal melanocytes or rounded cells and their presence may be directly related to the deposition of mucopolysaccharide in the dermis. In some cases they may invade the deeper tissues. Two cases of stiff skin syndrome that were in the Japanese literature are also discussed.

Adolescent↗
PubMed1985

In vivo and in vitro effects of angiotensin II on the release of beta-endorphin-like immunoreactivity.

The effect of angiotensin II (A II) on the release of beta-endorphin-like immunoreactivity (beta-END-LI) in rats was studied in vivo and in vitro. Intravenous injection of 1 microgram/100 g body weight of A II resulted in significant increase in the plasma beta-END-LI level after 10 and 20 min. Intraventricular injection of 1 ng/100 g body weight of A II also resulted in significant increase in the plasma beta-END-LI level after 10 min. A II at concentrations of 10(-12) M-10(-10) caused dose-dependent stimulation of beta-END-LI release from dispersed cells of rat anterior pituitary. On gel chromatography, the beta-END-LI released by incubation of the cells with 10(-10) M A II separated into two components which eluted in the same positions as human beta-lipotropin and human beta-endorphin, respectively. The ratio of beta-LPH to beta-END in these fractions was 5:1 on a molar basis. A II did not stimulate beta-END-LI release in Ca++-free-medium. [Sar1, Ala8]-A II at concentrations of 10(-9) M - 10(-7) M did not stimulate beta-END-LI release from the cells. Addition of [Sar1, Ala8]-A II to the incubation medium inhibited A II-induced beta-END-LI release from the cells. These results indicate that A II acts, at least in part, directly on anterior pituitary cells to stimulate beta-END-LI release and that calcium ion is involved in the mechanism of this effect.

Angiotensin II↗
PubMed1985

Effect of o,p'-DDD on cortisol metabolism in Cushing's syndrome of various etiology.

Effects of o,p'-DDD on parameters of cortisol metabolism were studied in 3 patients with Cushing's syndrome (ectopic ACTH-syndrome, Cushing's disease, and adrenal cancer). Before o,p'-DDD treatment, plasma cortisol, urinary 17OHCS, and urinary free cortisol were elevated in all patients. These parameters correlated well with each other in ectopic ACTH-syndrome and Cushing's disease. However, in adrenal cancer, urinary 17OHCS did not correlate with either plasma cortisol or urinary free cortisol, while the latter two parameters did. During o,p'-DDD, urinary 17OHCS rapidly declined in a patient with ectopic ACTH syndrome and a patient with Cushing's disease before plasma cortisol or urinary free cortisol decreases. Consequently the positive correlations of urinary 17OHCS with the other parameters were lost. In a case of adrenal cancer, urinary 17OHCS again did not correlate with plasma cortisol or urinary free cortisol. In these conditions, plasma cortisol and urinary free cortisol still significantly correlated. The present results demonstrated the limit of urinary 17OHCS as the index of the cortisol secretion rate both in some cases of adrenal cancer and in patients taking o,p'-DDD. It is suggested that urinary free cortisol should be utilized as a more accurate index for the cortisol secretion rate in such circumstances.

17-Hydroxycorticosteroids↗