The coexistence of substance P with neurotensin-like immunoreactivity in certain neurons of the hypothalamus were demonstrated by the double immunofluorescence method. Substance P and neurotensin-like immunoreactivity coexisted within single neurons of some hypothalamic areas such as the medial preoptic area, perifornical area, anterior hypothalamic area, lateral hypothalamic area, periventricular nucleus and posterior hypothalamic nucleus, although they did not coexist in the majority of immunoreactive cells.

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PubMedDate not supplied

The colocalization of substance P- and somatostatin-like peptides in neurons of the entopeduncular nucleus of rats.

The colocalization of substance P-like and somatostatin-like immunoreactivity in cell bodies was investigated in the entopeduncular nucleus of rats by a double immunofluorescence method using species specific antibodies. Most of the substance P-like immunoreactive cells were also positive to somatostatin and mainly seen in the rostral to middle region of the entopeduncular nucleus. Therefore it is suggested that double-labeled neurons in the entopeduncular nucleus project to the lateral habenular nucleus which is involved in the limbic system, since the rostral portion of the entopeduncular nucleus has been shown to project to the lateral habenular nucleus.

Animals↗

PubMedDate not supplied

Posterior lens rupture in rats.

Rupture of the posterior lens capsule with extrusion of lens fibers or entire lens cortex and nucleus was found in Sprague-Dawley rats. The lesion was detected during an ophthalmoscopic examination as round shadows that interfered with the view of the fundus. By slit-lamp biomicroscopic and dissecting microscopic examinations, the shadows were related to opaque plaques located on the posterior capsule. By light microscopic examination, the posterior lens capsule was ruptured and lens fibers extruded into the vitreous. Mononuclear and multinucleated giant cells were present at the site of rupture, however, few other inflammatory responses were present. Although the incidence and pathogenesis of the lesion is unknown, the lesion should be considered along with other spontaneous ocular changes when evaluating safety assessment studies.

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