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Biomedical subjects

S INOUE

Publications and source records attributed to S INOUE.

At least 19 recordsLinked to original sources
PubMed1964

EFFECTS OF CUTANEOUS AND MUSCLE SENSORY NERVE VOLLEYS IN AWAKE CATS: A STUDY IN PERCEPTION.

The hypothesis that discharges from afferent nerves from muscle stretch receptors do not participate in kinesthesis has been substantiated by test of discrimination thresholds. In awake, unrestrained cats, nerve stimulation activating group I and most group II sensory fibers (from muscle spindles and Golgi tendon organs) in pure muscular nerves failed to evoke sensory discrimination. Cutaneous nerve stimulation in the same animals produced sensory discrimination even below intensities required to elicit detectable nerve thresholds.

Animals↗
PubMed1964

ISOLATION AND PROPERTIES OF INTACT MITOCHONDRIA FROM SPHEROPLASTS OF YEAST.

Duell, E. A. (Western Reserve University, Cleveland, Ohio), Sakae Inoue, and Merton F. Utter. Isolation and properties of intact mitochondria from spheroplasts of yeast. J. Bacteriol. 88:1762-1773. 1964.-Functionally intact mitochondria can be obtained in good yields by osmotic disruption of spheroplasts formed from yeast by treatment with an enzyme mixture from the snail digestive tract. The useful range of this method is extended greatly by pretreatment of the yeast cells with 2-mercaptoethylamine and ethylene-diaminetetraacetate. The concentration of the yeast suspension can be increased greatly, the incubation period can be shortened considerably, and the requirement for log-phase cells is obviated. Mitochondria prepared by this method have been compared with those obtained by mechanical disruption in terms of respiratory control, specific activity on a wide range of oxidizable substrates, heterogeneity during centrifugation, and structures observed by electron microscopy. In all cases, the mitochondria obtained from spheroplasts appear to be much less damaged by the preparative procedures.

Centrifugation↗