Biomedical subjects
Publications and source records attributed to S I Shereshkov.
The clinical course and some immunohematologic parameters of the blood and bone marrow were investigated in chronic neutropenia children with a high break of granulocyte maturation. Heterogeneity of etiopathogenetic mechanisms of the disease has been recorded in this group of children.
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Native and cryopreserved human embryo liver cells were stored up to be used in cancer patients with antitumor treatment-induced myelodepression. In the course of conservation and before administration, cells were bacteriologically tested after the USSR Ministry of Health standing procedures as well as in some additional serum media. As a result, both classical bacterial forms and their cell wall-degenerate organisms were identified. Contamination of biopreparations with the latter is hazardous for immunodepressive patients.
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Three types of human colonic carcinoma were studied in organ cultures. Apart from the formation of glandular structures and processes not structurally differing from those in human bodies, there occurred complexes of cancer cells spreading beyond the boundaries of the explant and forming no structures at all. It was shown that in organ cultures, cancer cells retained their capacity to synthesize carcinoembryonic antigen.
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It was shown by the method of multiple organ cultures on millipore filters that hemopoiesis preferably of erythroid type remained for more than one and a half months in the human embryo liver culture. General morphology of 7--50-day cultures was studied and described. Myeloid population of the cells (CFU conut) was exhausted practically by the 14--16th days of cultivation.
It was shown by the modified method of agar cultures that in the peripheral blood of a healthy man, aged from 4 days to 40 years. The number of cell precursors of granulocytes and macrophages (CFU-C) varied from 0.05 to 6.38 in adults and from 0.2 to 2.9 in children per 105 nuclear cells. CFU-C content in the patients with infectious mononucleosis and acute leukemia was 0.5--14 and 0--0.3 per 105 nuclear cells, respectively.
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