Biomedical subjects

S Huang

Publications and source records attributed to S Huang.

At least 307 records · Page 17Linked to original sources

PubMed1998

Overexpression of 20-oxidase confers a gibberellin-overproduction phenotype in Arabidopsis.

In the gibberellin (GA) biosynthesis pathway, 20-oxidase catalyzes the oxidation and elimination of carbon-20 to give rise to C19-GAs. All bioactive GAs are C19-GAs. We have overexpressed a cDNA encoding 20-oxidase isolated from Arabidopsis seedlings in transgenic Arabidopsis plants. These transgenic plants display a phenotype that may be attributed to the overproduction of GA. The phenotype includes a longer hypocotyl, lighter-green leaves, increased stem elongation, earlier flowering, and decreased seed dormancy. However, the fertility of the transgenic plants is not affected. Increased levels of endogenous GA1, GA9, and GA20 were detected in seedlings of the transgenic line examined. GA4, which is thought to be the predominantly active GA in Arabidopsis, was not present at increased levels in this line. These results suggest that the overexpression of this 20-oxidase increases the levels of some endogenous GAs in transgenic seedlings, which causes the GA-overproduction phenotype.

Arabidopsis↗

PubMed1998

Temporal gene regulation of the channel catfish virus (Ictalurid herpesvirus 1).

To identify promoter regions that impart differential temporal regulation of channel catfish virus (CCV) genes, the transcriptional kinetics of an immediate-early gene and prospective early and late genes were characterized. A cDNA clone, designated IE3C, representing a third immediate-early transcript was identified. The 5' end of the IE3C transcript was mapped to nucleotides 15,368 and 131,043 in the terminal repeat regions of the CCV genome. The full length of the transcript represented by the IE3C clone is 1,412 bp, and it most likely codes for the protein specified by open reading frame (ORF) 12. The putative product of ORF12 contains a consensus RING finger metal binding motif (C3HC4 structure). Temporal expression studies, in conjunction with protein synthesis and DNA replication inhibition, demonstrated that the IE3C transcript belongs to an immediate-early kinetic class, the ORF5 transcript is a member of the early kinetic class, and ORF39 and ORF46 are true late-kinetic-class genes. Additionally, we demonstrated that ORF38 transcription overlaps ORF39 and the products presumably share the same poly(A) signal. The 5' ends of the transcripts encoding ORF38, ORF39, and ORF46 were mapped to nucleotides 44,862, 45,254, and 59,644, respectively, and potential transcriptional control elements were located.

Amino Acid Sequence↗

PubMed1998

Adenovirus internalization and infection require dynamin.

The cell receptors that facilitate adenovirus internalization into cells have been identified; however, the infectious pathway of virus entry has not been established. Adenovirus entry and infection were examined in HeLa cells lacking or overexpressing mutant dynamin, a protein that specifically regulates clathrin-mediated endocytosis. Expression of mutant dynamin significantly reduced adenovirus internalization and gene delivery, indicating a functional requirement for this molecule. These findings are consistent with virus entry via the clathrin-coated pit pathway.

Adenoviruses, Human↗

PubMed1998

Identification of a human immunodeficiency virus type 2 (HIV-2) encapsidation determinant and transduction of nondividing human cells by HIV-2-based lentivirus vectors.

Although previous lentivirus vector systems have used human immunodeficiency virus type 1 (HIV-1), HIV-2 is less pathogenic in humans and is amenable to pathogenicity testing in a primate model. In this study, an HIV-2 molecular clone that is infectious but apathogenic in macaques was used to first define cis-acting regions that can be deleted to prevent HIV-2 genomic encapsidation and replication without inhibiting viral gene expression. Lentivirus encapsidation determinants are complex and incompletely defined; for HIV-2, some deletions between the major 5' splice donor and the gag open reading frame have been shown to minimally affect encapsidation and replication. We find that a larger deletion (61 to 75 nucleotides) abrogates encapsidation and replication but does not diminish mRNA expression. This deletion was incorporated into a replication-defective, envelope-pseudotyped, three-plasmid HIV-2 lentivirus vector system that supplies HIV-2 Gag/Pol and accessory proteins in trans from an HIV-2 packaging plasmid. The HIV-2 vectors efficiently transduced marker genes into human T and monocytoid cell lines and, in contrast to a murine leukemia virus-based vector, into growth-arrested HeLa cells and terminally differentiated human macrophages and NTN2 neurons. Vector DNA could be detected in HIV-2 vector-transduced nondividing CD34(+) CD38(-) human hematopoietic progenitor cells but not in those cells transduced with murine vectors. However, stable integration and expression of the reporter gene could not be detected in these hematopoietic progenitors, leaving open the question of the accessibility of these cells to stable lentivirus transduction.

Antigens, CD34↗

PubMed1998

[Thiamine status of university teacher families in Changsha].

To evaluate the thiamine status of the population consuming the refined cereals and its products, we studied thiamine concentration in 23 food samples and human urine, and three-day dietary survey was conducted. The results showed that the thiamine content in the staple food was much lower than that in the raw material. The thiamine intake from diet was below to 80% RDA in 42.8% investigated families and the thiamine energy ratio was lower than 0.5 mg/4186.8 kJ in 71.5% of them. For the ratio of urinary tiamine (mg)/urinary inosine (g), 51.3% subjects exhibited decreased excretion, especially in the school-age children. We conclude that those people who take the refined cereals and its products as a staple food, is in the risk of thiamine deficiency.

Adolescent↗

PubMed1998

[Immuno-pathomorphological study of placenta with anticardiophospholipid antibody positive].

OBJECTIVE: To investigate the relationship between the anticardiophospholipid antibody (ACA)-positive cases and the poor pregnancy outcomes by observing the immuno-pathomorphology of the placenta. METHODS: 16 cases of ACA-positive placenta (8 normal and 8 poor pregnancy outcomes) were collected as the studied group, and 8 cases of ACA-negative placentas served as the controlled group, and immunofluorescence technique (IFT) was used. RESULTS: In the studied group, the deposits of immuno-complex were found in the cytoplasm of trophoblast and on the walls of villous blood vessels. IgA manifested as filiform, and IgG, IgM, C3 and C4 appeared as filiform, granular and tubercular as well. In contrast, all the immuno-globulins displayed negative reaction in the controlled group, and C4 demonstrated some small focal positive reaction. The frequency of ACA-positive in IUGR cases were 19.30% (22/144), and of the IUGR in all the ACA-positive pregnant women was 15.28%(22/144). There were 3 cases of IUGR complicated by severe pregnancy induced hypertension, 2 cases of prematurity and 5 newborns with serum ACA IgG positive. CONCLUSION: The poor pregnancy outcome in ACA-positive women is related to the dysfunction of autoimmuno-system, and ACA-positive may be one of the causes of IUGR. The immuno-injury occurred in the placenta may affect the normal function of placenta. Maternal antibody may be transmitted to the fetus, and its significance needs further study.

Antibodies, Anticardiolipin↗

PubMed1998

[Detection of intrauterine fetal growth of twins].

OBJECTIVE: To investigate how to detect intrauterine fetal growth of twins with height of uterine fundus. METHODS: This study was a randomized coupling trial. 75-couples of twins and single gestation were involved. Linear regression analysis was made after correcting the uterine height of single gestation. RESULTS: If each of twins develops as the same as single gestation, the relationship in uterine height between twins and single gestation can be described as a correlation equation: y = 1.26x + 1.3 (R2 = 0.656, r = 0.81, P < 0.001). The regression coefficient is 1.26, which is very closed to 3 square root of 2. According to the correlation equation, we made a table to forecast the uterine height of twins and single gestation in different gestational age. CONCLUSION: The ralationship in the uterine height between twins and single gestation can be used to predict intrauterine fetal growth of twins.

Adult↗

PubMed1998

[Nitric oxide inhalation in the treatment of acute respiratory distress syndrome].

OBJECTIVE: To investigate the role of nitric oxide (NO) in acute respiratory distress syndrome (ARDS). METHODS: Ten patients with ARDS underwent Swan-Ganz catheterization and mechanical ventilation. Before and after inhalation of 35 ppmNO, hemodynamic, blood gas and DO2 parameters were examined. RESULTS: After inhaling NO, mPAP and PVR were significantly reduced from 3.6 +/- 0.6 kPa to 2.7 +/- 0.5 kPa and from 32 +/- 11 kPa.s-1.L-1 to 22 +/- 9 kPa.s-1.L-1 respectively (P < 0.001). CO, BP and SVR were not significantly different (P > 0.05). Qs/Qt and P(A-a)O2 were decreased from 31% +/- 9% to 25% +/- 8% and from 37 +/- 6 kPa to 34 +/- 6 kPa respectively (P < 0.01). PaO2 and SaO2 were increased from 12 +/- 3 kPa to 15 +/- 5 kPa and from 95% +/- 3% to 96% +/- 3% respectively (P < 0.05, P < 0.001). CONCLUSION: In the treatment of ARDS, inhalation of NO can have a better effect.

Administration, Inhalation↗

PubMed1998

[Studies on red orpiment induction of NB4 and HL-60 cell apoptosis].

OBJECTIVE: To study the possible mechanism of red orpiment, which is main component of composite indigo naturalis tablets, in the treatment of acute promyelocytic leukemia(APL). METHODS: The effect of red orpiment on induction of APL cell line NB4 and HL-60 apoptosis were studied by cell morphology, DNA gel electrophoresis and flow cytometry assay. RESULTS: Red orpiment induced NB4 and HL-60 cell apoptosis. When treated with different concentration of red orpiment(25-200 micrograms/ml) for 16 hours, both NB4 and HL-60 cells showed typical apoptosis features. If decreased the concentration of red orpiment to 12.5 micrograms/ml, the NB4 cell still showed apoptosis features while the HL-60 cell did not when cultured for 72 hours. Arsenic disulfide(As2S2) had the same effect as red orpiment did under the same experiment condition. CONCLUSION: It is the main component, As2S2 of the red orpiment that can induces NB4 and HL-60 cell apoptosis. and the red orpiment is responsible for the high CR rate of APL induced by the composite indigo naturalis tablets.

Antineoplastic Agents↗

PubMed1998

[Aberrant expression of immunoglobulin germline gene C mu in leukemias].

OBJECTIVE: To investigate the significance of immunoglobulin germline gene C mu expression in leukemias. METHODS: Immunoglobulin germline gene C mu transcripts in 63 bone marrow samples from 59 leukemia patients were examined by RT-PCR. RESULTS: Germline C mu transcripts could be identified in 17 of 26 AML, 9 of 12 ALL, 4 CML-BC and 2 CLL patients. HLA-DR was expressed in 80% C mu(+) patients and in 92.9% C mu(+) AML patients. In 10 normal bone marrow or peripheral blood samples C mu transcripts were undetectable. CONCLUSION: Leukemogenesis in most acute leukemia may originate from the common progenitors of early B-lymphoid and myeloid cells.

Acute Disease↗

PubMed1998

[A pathological study on obliterative arteriopathy in human renal allografts].

OBJECTIVE: To observe the histopathological changes of obliterative arteriopathy (OBA) in human renal allografts, analyse OBA cellular components and probe its pathogenesis. METHODS: 74 renal allografts removed due to acute vascular rejection and chronic rejection were studied by light microscopy, electron microscopy and immunohistochemistry methods. RESULTS: OBA predominately affected the intima of interlobar, arcuate and interlobular arteries in all the 74 renal allografts. 29 cases developed OBA within 3 months post transplantation. 19 (25.7%) renal allografts showed early OBA with intimal arteritis, 55 (74.3%) cases had advanced OBA with intimal spindle-shaped smooth muscle cell (SMC) proliferation and intimal fibrosis. The cellular composition of OBA consisted of two origins, one from the host and the other from arterial wall intrinsic cells of renal allografts, including T lymphocytes; monocyte/macrophage cells; endothelial cells and different phenotypes--"contractile" or "synthetic" SMCs, "synthetic" SMCs secreted collagen types I, III. CONCLUSION: Our findings suggest that OBA is a post transplantation intrarenal arterial wall proliferative lesion. It is the result of a series of interactions between host immunocytes and allograft vascular wall cells. OBA is a type of immune reaction.

Graft Occlusion, Vascular↗

PubMed1998

[Correlation between human papillomavirus type and grading of cervical intraepithelial neoplasia].

OBJECTIVE: To disclose the correlation between human papillomavirus (HPV) type and grade of cervical intraepithelial lesion (CIN). METHODS: PCR and in situ hybridization (ISH) techniques were used to detect the presence of HPV 6B/11, 16, 18 in 61 cases of CIN and 12 cases of cervical squamous cell carcinoma (SCC). RESULTS: PCR showed HPV 6, 11 distributing mainly in low-grade squamous intraepithelial lesion (LSIL, 61.9%) and partly in CINII (20%), but not in CINIII and SCC. The frequency of HPV 16, 18 increased corresponding to the increasing of CIN grading and up to 83.3% in SCC. ISH showed HPV 6b/11, 16, 18 DNA hybridization intranuclear signal were diffusely distributed in LSIL and granular in appearance in episomal HPV. Similar signal were also seen in CINII infected by HPV 6b/11 and partly by HPV 16, 18, anyhow, CINIII, SCC and partly CINII HPV 16, 18 DNA hybridization were nongranular in appearance. CONCLUSION: low-grade SIL associated with "low-risk" types of HPV 6, 11 belonged to productive infection. CINIII and SCC associated with "high-risk" types of HPV 16, 18 belonged to integrated infection, while CINII belonged to either productive or integrated infection.

Carcinoma, Squamous Cell↗

PubMed1998

[Clinical analysis of syndrome of obstructive sleep apnea (OSA) induced acute respiratory failure].

OBJECTIVE: To study the clinical characteristics of syndrome of OSA induced acute respiratory failure that might allow earlier recognition and treatment in critical care. METHOD: The clinical and laboratory characteristics of 9 patients were reviewed. RESULT: 9 patients (8 female, 1 male) presented with obesity and mental disturbance, with a BMI being 44.97 kg/m2, (45.25 kg/m2 in the female). The mean age of the group was 67.89 years (61-74 y). All had respiratory acidosis (mean pH 7.1732), hypercapnia (mean PaCO2 12.56 kPa) (8.53-19.09 kPa), and hypoxemia (mean PaO2 5.20 kPa) (3.87-5.87 kPa). During period of clinical stability all but 2 had awake hypercapnia (mean PaCO2 6.23 kPa) (5.10-7.29 kPa). Four of the 9 patients had pulmonary function test showing FEV1% > 70%. CONCLUSION: OSAS induced acute respiratroy failure has a sudden onset and various presentation and can be reversed with early and proper treatment. The severity of abnormal pulmonary function was less than what would be expected to cause respiratroy failure.

Acidosis, Respiratory↗

PubMed1998

[Comparison of continuous positive airway pressure (CPAP) with uvulopalatopharyngoplasty in the treatment of obstructive sleep apnea syndrome].

OBJECTIVE: To compare the efficacy, side effects, and effects on parameters of sleep of continuous positive airway pressure (CPAP) and uvulopalatopharyngoplasty (UPPP) therapy in OSAS and evaluate their role in the treatment of OSAS. METHOD: 60 patients with OSAS were studied, 36 patients treated with CPAP, 24 patients with UPPP, and the parameters of polysomnography (PSG) obtained before and after treatment were analysed. RESULT: Respiratory disturbance index and sleep hypoxemia were improved significantly in both groups, but the CPAP group was better than UPPP group (P < 0.01). The efficacy was 97% in CPAP and 46% in UPPP group (P < 0.01). The longest apnea duration shortened in CPAP group (from 89 +/- 46 to 39 +/- 19) but there was no difference in UPPP (from 76 +/- 26 to 62 +/- 30) group. The apnea duration in 10 patients was lengthened. CONCLUSION: The effects of UPPP in OSAS were limited. CPAP was more effective than UPPP. CPAP may be indicated as the first choice in the treatment of OSAS.

Adult↗

PubMed1998

[Relationship between Syndrome-Type in TCM and four trace urinary proteins in patients with bronchial asthma].

OBJECTIVE: To explore the possible relationship between Syndrome-Type in TCM and the 4 trace urinary proteins in patients with bronchial asthma. METHODS: The 4 kinds of trace urinary proteins in 217 patients with asthma were measured by radioimmunoassay, and that in 66 normal subjects were compared. RESULTS: The results showed that there were increments of urinary albumin, urinary IgG, urinary beta 2-microglobulin and urinary Tamm-Horsfall protein in patients with asthma; and the gradual increase tended to be accompanied by the continuous aggravation of patient's condition on TCM Syndrome-Type. Furthermore, these datas proved that the 4 trace urinary proteins might be the most sensitive indicators for detecting impaired renal functions than serum blood urea nitrogen and creatinine. CONCLUSIONS: There were the objective material foundations on TCM Syndrome-Type in asthmatics, which consisted of renal glomerular dysfunctions to filter out albumin and IgG, renal tubular dysfunction to reabsorb beta 2-microglobulin, and to synthesize and excrete Tamm-Horsfall protein.

Adolescent↗

PubMed1998

[Intraventricular central neurocytoma and literature review].

OBJECTIVE: To understand the characteristics of central neurocytoma. METHODS: Two cases were confimmed operatively and pathologyically. RESULTS: 142 cases showed that the tumor is common in young people. It occurrs in the septum pellucidumor, the wall of third or lateral ventricle. Clinical symptom is intracranial hypertension. The diagnosis must depend on electromicroscope examination. CONCLUSION: The tumor must be removed totally by microsurgery.

Adult↗

PubMed1998

Cloning and characterization of a metal responsive element-containing fragment from the Wilson disease gene locus by junction trapping.

All mammalian metallothionein genes studied to date have several metal responsive elements (MRE) with consensus sequences of TGCRCNC (R, purine) in their regulatory region. MRE-like sequences were also found in many other metal-related genes. To see whether there is also such a sequence at the genetic locus (13q14.3) of Wilson disease, which is a genetic disorder of copper metabolism, junction-trapping method based on the MRE sequence was used. A fragment containing MRE and MRE-like sequences from YAC 27D8 at the WND locus was successfully cloned and mapped back to the YAC by PCR. Presence of such a sequence in the copper transporter gene at the WD locus might imply that it has a possible interesting role in the regulation of WD gene expression.

Adenosine Triphosphatases↗

PubMed1998

[Examination of anterior proliferative vitreoretinopathy with ultrasound biomicroscopy].

OBJECTIVE: To diagnose anterior proliferative vitreoretinopathy (a-PVR) with ultrasound biomicroscopy (UBM). METHODS: The UBM images of 27 cases (27 eyes) of suspicious a-PVR from cases with retinal detachment with PVR were analyzed and compared with the images of B scan ultrasound and the findings during vitrectomy. RESULTS: UBM diagnosis of a-PVR was found in 20 eyes with retinal circumferential contraction, and in 15 eyes with retinal anterior displacement. A-PVR was not diagnosed by UBM in 7 eyes. UBM images of a-PVR included: the pattern of dots and cords in vitreous proliferation, the echo augmentation and obvious central displacement of the retina with circumferential contraction. Besides, the trough, "angle" and "Z" shaped overlap of the retina were the characteristics of the retinal anterior displacement. CONCLUSION: UBM plays an important role in the clinical diagnosis of a-PVR.

Adolescent↗