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Biomedical subjects

S Hosaka

Publications and source records attributed to S Hosaka.

At least 73 records · Page 4Linked to original sources
PubMed1993

Muscle pH/PCO2 monitoring.

The surface pH of skeletal muscle has been confirmed to be a sensitive indicator of peripheral muscle blood flow, but the development of a better electrode has been required. As a consequence, a unique and minute sensor was developed to measure muscle pH/PCO2. The purpose of this article was to evaluate this new monitoring device experimentally and clinically in order to clarify its effects, usefulness and specificity. Our experimental and clinical results showed that placement and measurements using the muscle pH/PCO2 monitor were easily and quickly accomplished without any complications. This small sensor provided an accurate assessment of vital physiologic functions and gave an early warning of clinical deterioration. The new monitor should be useful in pediatric surgery clinical management.

Animals↗
PubMed1993

[Continuity of care during hospital and after hospitalization for the patients with stroke. Comparing the level of independence in ADL and care needs].

Thanks to the advancement of medical technology, there has been a significant decline in stroke mortality in Japan. This means that many stroke victims could gain additional years of survival with several disabilities. It is necessary that nurses assist these disabled patients upon discharge for shifting smoothly from the hospital to the home. The purpose of this study was to clarify the factors of continuity of care for the disabled patients who has had a stroke, between hospital and community. The 504 patients who were discharged from the Kanagawa Prefectural Rehabilitation hospital and were in need of visiting nursing services upon their discharge were selected in this study. The survey was conducted through discharge summaries and visiting nursing reports. The data were compared at discharge with the first nursing visit with respect to the level of independence in activities of daily living (ADL) and their care needs. The results were summarized as follows: 1. As for the functional level of ADL, most patients remained unchanged. Feeding was improved; however, grooming (including bathing), using the toilet, and dressing were worse. Hence, hospital nurses should strongly educate the patients and repeat ADL so that dressing is a rehabilitation exercise itself. Also, nurses, prior to discharge, should consider dealing with the housing problems, especially in the bathrooms and washrooms, due to the patients' physical disabilities. Nurses should pay particular attention to the aged group upon the continuity of care since their level of independence in walking changed variously among individuals. 2. Hospital nurses identified nursing problems mostly in the physical aspects, whereas public health nurses did it in terms of family-caregivers and social resources rather than physical needs. The reasons why nursing problems did not correspond between hospital nurses and public health nurses were probably owing to the differences in their practice settings, job content, and working conditions between the hospital and community. Thus, both nurses should reciprocally provide one's information and introduce other's information in order to understand and employ the other's standpoints.

Activities of Daily Living↗
PubMed1993

[Usefulness of Vmax and time constant in the evaluation of left ventricular function immediately after cardiac operation].

It is difficult to evaluate the cardiac function accurately, immediately after cardiac operation, because of the restriction of measurements and the variations of loading. To evaluate the exact cardiac function during and after cardiac surgery, we examined the usefulness of unloaded maximal shortening velocity of contractile element (Vmax) and time constant of isovolumic pressure fall (TC) in the condition of hasty changing of preload and afterload. In our experiment, we used canines as our subject. Following the sedation of the canines, the left ventricular pressure (LVP) curve was recorded by using the catheter-tip manometer which was introduced into the apex of the heart. The LVP was measured in the following instances: 1) at control condition, 2) 5 minutes after the administration of dobutamine of 2 or 5 micrograms/kg/min, 3) 5, 10, 15, and 30 minutes interval after the ligation of the left anterior descending artery (LAD). In every situations, preload and afterload were changed by the temporary occlusion of the inferior vena cava or descending thoracic aorta. Vmax and TC were analyzed easily only from the LVP by the personal computer. We used Grunkemeier's method to calculate Vmax which was analyzed only from the LVP curve. We presumed that the mean value of Vmax analyzed by Grunkemeier's method was almost the same as the data by Mason's method by calculating the mean value from the given number of heart beats. Because the correlation of two methods was linear, and furthermore the slope of this regression line was 0.97 and the Y-intercept was 0.19. Vmax was the index that was comparatively independent from the loading condition.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1993

[Computerized recording system during cardiopulmonary bypass utilizing the "GEM 6 PLUS analyzer"].

We developed a new computerized recording system to reduce artificial errors by perfusionists during cardiopulmonary bypass. This system included the efficient functions to record the progress of perfusion flow, perfusion pressure, urine output and temperatures from several sources. Furthermore, it could automatically record considerable data of the "GEM 6 PLUS analyzer" which was developed for monitoring of pH, PCO2, PO2, BE, Ca2+, K+ and hematocrit during cardiopulmonary bypass. We considered it very important that the errors of hematocrits associated with electrolytic concentrations. Therefore, we corrected the hematocrit according to a formula based on our experiments, i.e., HCT = GEMHCT + 0.28 x ([Na+]+[K+]) -44. And also we expect that the "GEM 6 PLUS analyzer" will be improved to measure Na+ value in near future.

Body Temperature↗
PubMed1992

Adult Still's disease with myocarditis and peritonitis.

A 26-year-old woman had myocarditis and peritonitis during an acute multisystem attack of Still's disease. To our knowledge, these complications are rare manifestations of adult Still's disease. Treatment with high-dose adrenocorticosteroids was rapidly successful in controlling these manifestations.

Adult↗
PubMed1992

[Chest wall reconstruction with a prosthetic material molded from a extirpated specimen].

A new molding technique of the Acryl-resin Marlex-mesh sandwich method is described. After the wide extirpation of the chest wall, the defect size tends to grow up more larger than the specimen. For making a just fit size prosthesis, we cut off a cardboard as the same size as the specimen and the Acryl-resin Marlex sandwich is molded from the cardboard. Other some know-how are in the text.

Acrylic Resins↗
PubMed1991

Follicle-like hemicysts reorganized in monolayer culture of rat thyroid cells in the medium supplemented with isologous serum.

Monolayer culture of the cells isolated from rat thyroid glands formed a number of large and three-dimensional (about 200-microns diameter) hemicysts in media supplemented with isologous and high-concentration serum. The higher was the concentration of isologous serum, the larger was the hemicysts and the higher was the uptake of 131I of hemicysts. Substitution of heterologous serum, such as calf or fetal calf serum, for the isologous serum failed to form hemicysts.

Animals↗
PubMed1991

Dynamic movement of hemicysts reconstructed from monolayer cultures of rat thyroid cells in isologous serum.

Time-lapse studies using cine-microscopic observation showed that the hemicysts formed in a culture of rat thyroid cells in a high concentration of isologous serum were found to arise by swelling from the monolayer sheet, gradually increase in size, and then rapidly collapse. The hemicyst volume increased linearly. We speculate that each hemicyst epithelium transport a constant volume of liquid into the hemicyst lumen.

Animals↗
PubMed1991

[Nonocclusive intestinal necrosis after the successful veno-arterial bypass].

A 52-year-old male suffered from acute viral myocarditis with severe cardiogenic shock. On his admission, he underwent the inotropic and IABP supports, and subsequently was treated effectively by V-A bypass, which was continued for 35 hours. But he died in the period of 69 hours after V-A bypass from extended bowel necrosis and septic shock. The small and large intestinal mucosal necrosis, hemorrhagic hepatic necrosis and acute tubular necrosis were confirmed at necropsy. It is well known that the nonocclusive mesenteric vascular spasm is concerned strongly in their pathogenesis, after prolonged severe cardiogenic shock.

Acute Disease↗
PubMed1991

[A case of Morgagni's hernia: availability of CT scan, ultrasonography, and trans-abdominal surgical approach].

A 60-year-old obese woman was admitted to our hospital to receive surgical treatment for "slow-growing mediastinal lipoma". With the non-invasive examination such as CT scan and ultrasonography, she was revealed as Morgagni's hernia containing a greater omentum. With a trans-abdominal approach, we could take a good surgical field for the bilateral foramina of Morgagni. Several discussions were described for availability of CT scan, ultrasonography and a trans-abdominal approach.

Diagnosis, Differential↗
PubMed1988

Effect of neutrophil activating substances on intracellular generation of phagocyte chemiluminescence by means of luminol-bound microspheres.

The intracellular chemiluminescence of granulocytes was measured by luminol-bound microspheres. After one minute of incubation, which was necessary for the granulocytes to phagocytize microspheres, intracellular chemiluminescence was generated. The intracellular chemiluminescence was enhanced by the introduction of granulocyte stimuli including concanavalin A, phorbol-myristate acetate, and formyl peptides. This incremental effect was the result of certain metabolic changes inside the cells. This newly discovered chemiluminescent reagent promises to be a useful tool for examining metabolic changes inside phagocytic cells.

Concanavalin A↗
PubMed1986

Measurement of active phagocytosis by polymorphonuclear leukocytes by fluorescence liberation from phagocytized microspheres.

Phagocytosis by polymorphonuclear leukocytes (PMN) was determined by a newly developed technique based on measurement of liberation of a fluorescence substance from PMN phagosomes; 4-methylumbelliferyl-beta-D-glucuronide (4MUGL), which is a substrate of beta-glucuronidase in lysosome, was conjugated with a microsphere, and 4-methylumbelliferone (4MU) liberated from phagocytized 4MUGL-microspheres was measured. The microspheres were composed of glyceryl-methacrylate having a diameter of 2.0 micron. Liberating activity of six kinds of 4MUGL-microspheres containing various amounts of amino and carboxyl groups was compared. Among these six kinds of 4MUGL-microspheres, four kinds showed activity similar to that of morphological phagocytosis. These four kinds of 4MUGL-microspheres liberated 4MU into the extracellular fluid from PMN during phagocytosis. Furthermore, they were recognized as a substrate of purified beta-glucuronidase. 4MUGL-MS610 showed the highest liberating activity among the four kinds of microspheres. Optimal conditions for phagocytosis by PMN were determined using 4MUGL-MS610. Total liberation of 4MU from the microspheres increased almost linearly with incubation time with PMN from 0 to 60 min and was linear with 4MUGL-MS in concentrations up to 4 X 10(8) microspheres/ml. This liberation was parallel to phagocytosis in a dose-dependent fashion. During 10-min incubation 20.4% of 4MU was liberated from 4MUGL-microspheres with phagocytosis. Seventy-five percent of the liberated 4MU was distributed in the extracellular fluid. 4MU distributed in the extracellular fluid was not attributable to hydrolysis of unphagocytized microspheres by beta-glucuronidase extracellularly leaked from PMN by phagocytosis. Also phagocytized 4MUGL-MS610 by PMN was observed by scanning electron microscopy. These results indicate that 4MU was liberated from 4MUGL-MS by hydrolysis due to beta-glucuronidase released into phagosomes with phagocytosis by PMN. Sensitivity of this assay was limited to about 50 pmol/ml, being less than 0.5-1 microsphere phagocytized into one cell.

Cell Membrane Permeability↗
PubMed1985

Direct measurement of phagolysosomal esterase activity.

A new method of directly measuring esterase activity within phagolysosomes has been developed. Decanoyl fluorescein- binding microspheres were prepared and phagocytosed by human peripheral neutrophils. Within phagolysosomes lysosomal esterase hydrolyzed decanoyl fluorescein on the microspheres, causing the conversion of decanoyl fluorescein- binding microspheres (non-fluorescent) into fluorescein- binding microspheres (fluorescent). The activity of phagolysosomal esterase in intact neutrophils was assayed by the measurement of the fluorescence intensity without rupturing cells. By use of a flow cytometer, esterase activity within phagolysosomes in single cells was measured.

Esterases↗
PubMed1985

Direct measurement of phagosomal reactive oxygen by luminol-binding microspheres.

A new method, utilizing microsphere-bound luminol, which makes possible the direct measurement of highly reactive oxygen within phagosomes, was studied. When Freund's complete adjuvant-elicited mouse peritoneal macrophages and luminol-binding microspheres were mixed, the microspheres were engulfed in macrophages and enclosed in phagosomes, where chemiluminescence (CL) was generated, showing the generation of highly reactive oxygen. The reactive oxygen could be quantitatively assayed by measuring the intensity of CL. The addition of cytochalasin B inhibited the CL. CL production by the thioglycollate-elicited macrophages was found to be only a ninth of that by Freund's complete adjuvant-elicited macrophages, though the phagocytic activities were almost equivalent in both cases.

Animals↗