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Biomedical subjects

S Hong

Publications and source records attributed to S Hong.

At least 145 records · Page 8Linked to original sources
PubMed1997

[Primary analysis of the situation of hepatitis G virus infection in different groups of people].

In order to investigate the situation of hepatitis G virus (HGV) infection among different groups of people, two self-selected peptides from HGV NS5 were used to develop an indirect ELISA for anti-HGV IgG detection. 1209 sera from different peoples were detected and the positive rate of anti-HGV IgG was (3.8%). Among these groups the group of non A-E hepatitis had the highest anti-HGV positive rate (20.5%), higher than that in the groups of natural people (0.8%) and other hepatitis patients (3.3%), the difference was significant. The anti-HGV IgG positive rate in the group of HC patients 8.0% was higher than that in other hepatitis A-E patients, the positive rate (8.0%) in paid donors was higher than that in voluntary donors (0%), and that in groups with venereal diseases and HIV infection were 3.6% and 8.0%, respectively. The results suggested that there has higher rate of HGV infection in our country; HGV is the important factor causing non A-E, the paid donors is the dangerous group, they should be strictly screened, and the people with transfusion have higher rate of HGV infection. Since there is different rate of HGV infection in different human population so it is important to study further the epidemiological characteristics of HGV for its prevention.

China↗
PubMed1997

[The detection of human immunodeficiency virus type 1/2 antibody using synthetic peptides].

Four peptides of human immunodeficiency virus (HIV) located in the regions of gp41, gp120, p24 of HIV-1 and gp36 of HIV-2 have been synthesized according to the published amino acid sequences and the positions of antigenetic determinants of viruses by a solid-phase method. An indirect ELISA for detecting antibody against HIV 1/2 was established using these synthetic peptides as the coating antigens. We detected 41 control sera provided by National Institute for the Control of Pharmaceutical and Biological Products. The results indicated that the general coincident rate were 100%, the variant coefficient was less than 10%. A comparison of our reagent with Huayi, Pasteur and Jinhao reagents in detection of 90 positive sera and 140 normal sera for HIV-1/2 showed that the coincident rate was 100% with Pasteur and Jinhao, 99.57% with Huayi. All of 186 sera from patients with other diseases were negative. The reagent are stable at 37 degrees C for 4 days. The results indicated that our reagent is highly specific, sensitive and stable, it can be used for detection of HIV-1/2 infections.

Antibodies, Viral↗
PubMed1997

[The changes of T lymphocyte subsets and serum interleukin-2 receptor in peripheral blood of the pregnant women complicated by systemic lupus erythematosus].

OBJECTIVE: To determine the changes of T lymphocyte subsets, natural killer (NK) cell and serum interleukin-2 receptor (SIL-2R) in peripheral blood of the pregnant women with systemic lupus erythematosus (SLE). METHODS: T lymphocyte subsets NK cell, and SIL-2R in peripheral blood were detected by flow cytometry (FCM) and enzyme-labeled immunosorbent assay (ELISA) in 14 cases of pregnant women complicated by SLE (SLE+NP), 18 cases of stationary phase SLE (SLE), 20 cases of normal non-pregnant women (NNP) and 20 cases of normal pregnant women (NP). RESULTS: The percentages of CD4+ cell were decreased significantly in SLE+NP group as compared with the other three groups (P < 0.01); and the ratio of CD4+/CD8+ was decreased significantly in SLE+NP group, as compared that in SLE group and in NNP group (P < 0.01). There were no difference in the number of NK cells among the four groups. The SIL-2R values were found to be increased significantly in the SLE+NP group, as compared with that of other three groups (P < 0.01). CONCLUSION: The observation on the changes of peripheral T lymphocyte subsets and SIL-2R may be helpful for monitoring progress of SLE during pregnancy.

Adult↗
PubMed1997

[Antigenicity of nine synthetic peptides of human immunodeficiency virus].

To establish a ELISA method to detect the infection of human immunodeficiency virus (HIV), we synthesized nine peptides ranging in length between 10-27 amino acid (aa) of HIV located in the regions of gp41, p24 of HIV-1 and gp36 of HIV-2, according to the published amino acid sequence and the position of antigenic determinants of viruses, by a solid-phase method. We detected 10 positive sera of HIV-1 and 4 positive sera of HIV-2 by a indirect ELISA using these synthetic peptides as the coating antigen. The results indicated that all of 10 serum specimens of HIV-1 were positive using SP1, 5, 6, and 7 derived from gp41, and SP7 was the best in antigenicity. The 4 serum specimens of HIV-2 were positive using SP8 and SP9 in the region of gp36 and their antigenicity to HIV-2 were similar, but the antigenicity of SP8 to HIV-1 was higher than that of SP9. All sera of HIV-1 and HIV-2 were positive using SP7 and SP8 as the mixed coating antigen. A comparison of our reagent with UBI reagent in detection of 60 positive and 96 negative control sera for HIV showed that the coincident rate for positive ness was 98.33% (59/60), for negative ness was 100%, and the general coincident rate was 99.36% (155/156). It is concluded that SP7 and SP8 as the mixed coating antigen are applicable in the diagnosis of HIV infection.

Acquired Immunodeficiency Syndrome↗
PubMed1996

Unexplained elevated maternal serum alpha-fetoprotein levels and pregnancy outcome in twins.

OBJECTIVE: To evaluate whether elevated maternal serum alpha-fetoprotein (MSAFP) levels in uncomplicated twin gestations are associated with an increased risk for pregnancy complications and adverse outcomes. METHODS: A retrospective data-base analysis was conducted of 267 women with twin pregnancies delivered between January 1988 and October 1994, of whom 42 had elevated MSAFP levels and 225 had normal levels. We evaluated rates of preterm delivery (defined as gestational age less than 34 weeks at delivery), birth weight less than 1500 g, twin-to-twin birth weight discordance, small for gestational age (SGA) infants (defined as birth weight below the tenth percentile for gestational age), and fetal malformations. Also assessed were potentially confounding variables such as obstetric and medical histories as well as sociodemographic factors. RESULTS: Among nulliparous women, an unexplained elevation in MSAFP levels was associated with a statistically significant increased risk for preterm delivery. Among multiparous women, this association is suggested by the data, but not significantly so. An unexplained elevation in MSAFP level was also associated with a significantly increased risk for birth weight less than 1500 g, but this was related to the increased rate of preterm births. No appreciably increased risk was apparent for SGA infants, fetal malformations (other than neural tube defects and abdominal wall defects), or twin-to-twin birth weight discordance. CONCLUSION: In twin pregnancies, an unexplained elevation in MSAFP level may increase the risk for preterm delivery but not other adverse pregnancy outcomes.

Adult↗
PubMed1996

Ethnic variation of cord plasma apolipoprotein levels in relation to coronary risk level: a study in three ethnic groups of Singapore.

In multiracial Singapore, the prevalence of coronary artery disease is highest in ethnic Indian and lowest in ethnic Chinese populations. Since susceptibility to coronary artery disease is closely associated with plasma lipid traits, we studied the cord blood lipid and apolipoprotein profiles of the three ethnic groups in Singapore to determine if ethnic differences in lipid profile are present at birth. The high-risk lipid traits of high LDL-cholesterol, triglycerides and apo B, low HDL-cholesterol and apo A-I were found to be highest in ethnic Indian and lowest in ethnic Chinese populations. This difference was concordant with the relative coronary mortality rates for their respective adult populations in Singapore.

Analysis of Variance↗
PubMed1996

[An experimental study on the treatment of fetal rabbits with intrauterine growth retardation].

OBJECTIVE: To investigate the effect of histocyte activators and vasodilators on the amino acids concentration in fetal rabbits with intrauterine growth retardation (IUGR). METHODS: The rabbits with IUGR were caused by passive smoking and treated with histocyte activators (adenosine triphosphatase, coenzyme A, cytochrome C, and vasodilators (injectio salviac molfiorrhizae composita, anisodamine). The effects of different remedies on the fetal plasma amino acids' concentration and the fetal development (i.e. birth weight, the weights of fetal liver and fetal brain) were studied. RESULTS: Both histocyte activators and vasodilators had favorable effects on the improvement of fetal development and the increase of fetal plasma amino acids' concentration. But the action of histocyte activators was stronger than vasodilators. CONCLUSIONS: The results suggested that vasodilators could improve the uterine-placental flow, and histocyte activators could enhance placental transfer of amino acids. But histocyte activators seem to be better than vasodilators.

Adenosine Triphosphate↗
PubMed1995

Electroosmotic control of chiral separation in capillary zone electrophoresis.

The resolution in capillary zone electrophoresis (CZE), with the assumption of diffusion control only, is strongly dependent on the direction and magnitude of electroosmotic flow. In fact, excellent separation resolution will be obtained if the electroosmotic flow is in the opposite direction of the electrophoretic migration. By applying various radial electric potential gradients across the capillary wall, the direct control of the zeta potential and the electroosmotic flow results in a great enhancement of chiral resolution in cyclodextrin-modified CZE. All 12 basic chiral drugs, including (+/-)-ephedrine, (+/-)-pseudoephedrine, (+/-)-norephedrine, (+/-)-epinephrine, (+/-)-norepinephrine, and (+/-)-isoproterenol, are separated and resolved within 22 min of the analysis time. There is no additional band broadening and dispersion introduced by the direct control of electroosmosis with the application of various radial electric potential gradients.

Amines↗
PubMed1995

Racial variation of factor VII activity and antigen levels and their correlates in healthy Chinese and Indians at low and high risk for coronary artery disease.

Plasma factor VII activity (FVIIc) is one of the independent risk factors of coronary artery disease (CAD) and is controlled by both genetic and environmental factors. South Asians including Indians have one of the highest prevalence and mortality rates from CAD while the Chinese have a much lower risk. Generally accepted risk factors cannot explain the high mortality from CAD in Indians. We examined two hundred and seventy seven Chinese (124 m, 153 f); and 216 healthy Indian (150 m, 66 f) adults for serum lipids; plasma FVIIc and FVIIag levels in order to examine racial variations of these and their correlates in these two populations. Both Indian men and women had significantly higher FVIIc levels (12% and 11%, respectively) than the Chinese even after adjustments of age, BMI and lipids (P < 0.01). In contrast, Indians had significantly lower plasma FVIIag levels than Chinese (8% and 9%, respectively in men and women; P < 0.01). Multiple linear regression analysis shows a strong correlation of FVIIc with serum triglycerides accounting for 4-8% of the total variability of FVIIc in different groups. Further, there was a stronger correlation between FVIIc and FVIIag in Indians than that in the Chinese (0.43 vs. 25) suggesting a greater activation resulting in higher FVIIc in Indians inspite of lower FVIIag levels. The higher FVIIc and stronger activation by triglycerides observed in this study partly explain the higher risk of CAD in Indians.

Adult↗
PubMed1995

Horseradish peroxidase permeability across rat nasal mucosa in selective stimulation of substance P innervation with capsaicin.

To investigate the physiological role of substance P innervation (SPI) in the nasal mucosa from inhaled irritant macromolecules, SPI in the rat nasal mucosa was selectively stimulated with capsaicin and the degree of horseradish peroxidase (HRP) permeability across the nasal epithelium was evaluated by enzyme immunoassay or by histochemical electron microscopy. The serum level of HRP was reduced significantly in capsaicin-administered rats compared to control rats along the time course with quantitative analysis of the enzyme immunoassay. Control rats showed heavy permeation of HRP across the epithelium, but capsaicin-administered rats showed weak permeation of HRP across the epithelium with histochemical electron microscopy. The epithelial lining and its tight junctions were left intact as judged by electron microscopy. In conclusion, selective stimulation of SPI of the rat nasal mucosa with capsaicin decreased the epithelial absorption permeability to HRP without compromising the epithelial integrity. These findings imply that the physiological role of capsaicin-sensitive SPI in the rat mucosa is to protect the airway from inhaled irritant macromolecules.

Alkaloids↗
PubMed1995

[The quantitative and morphological analysis of placenta and brain in rabbits with intrauterine fetal growth retardation].

OBJECTIVE: To observe the changes of quantitative and morphological structure in placenta and fetal brain of rabbits with intrauterine fetal growth retardation (IUGR) resulted from exposure to cigarette smoking. METHODS: The pure species of 23 pregnant New Zealand rabbits were used and divided into three groups. (1) group A: 9 rabbits were exposed to cigarette smoking everyday in the whole period of pregnancy; (2) group B: 7 rabbits were exposed to cigarette smoking everyday in the late half period of pregnancy; (3) group C: no smoking, as control group. The villus area of placenta, the capillary area in villus and the thickness of granulosa layer in fetal brain were studied by histomorphometric parameter. RESULTS: It was showed that the placental villus area was decreased significantly in group A than that of the control group. The capillary area in villus of both group A and group B was less than that of the group C. The thickness of granulosa layer in cerebrum and cerebellum of group B is less than that of the control group. Nevertheless, only the thickness of granulosa layer in cerebrum of group B decreased significantly than that of the control group. CONCLUSIONS: The development of cerebrum in fetal rabbits were influenced significantly by smoking. The development of cerebellum was influenced by smoking mainly in the early half period of pregnancy. It means that the most important time for cerebellum development is the early period of pregnancy and both the early and late half period of pregnancy were important for the cerebrum development.

Animals↗