By using thin-layer chromatography and infrared spectroscopy, xanthomegnin and viomellein have been isolated and identified from species of the Aspergillus ochraceus group. A correlation was established between the occurrence of these fungal quinones in the fungal cultural products and the ability of these products to induce mycotoxicosis in mice. In addition, a method was employed to estimate the amount of xanthomegnin and viomellein produced by the fungi.

Animals↗

PubMed1976

Electron spin resonance spin-label studies of intercalation of ethidium bromide and aromatic amine carcinogens in DNA.

A spin-labeled technique has been developed for the study of carcinogen-DNA intercalation mechanisms utilizing a modified ethidium bromide as a model ligand and specifically synthesized spin labels of carcinogenic aromatic amines such as 2-aminofluorene, 2-aminoanthracene, and 6-aminochrysene. The technique takes advantage of the characteristic electron spin resonance anisotropy of the corresponding spin labels complexed with DNA. Analysis of the orientation-dependent information thus obtained has clearly shown that the lignad moieties in all 4 respective complexes are accommodated in adjacent base-pair layers of the DNA with the nitroxide reporter oriented in 1 preferential direction. The geometry of the nitroxide rings with respect to their respective constraining ligands in a particular complex appeared to be largely dependent upon the size of the ligand moiety and the position of the reporter attachment on the ligand molecule. A coplanar configuration is most favored for the spin labels in which the ligand moiety per se is a 3-fused ring with the nitroxide substituted at a position on or close to the longitudinal end of the ligand molecule. Temperature studies in which the heat-induced release of the bound label is monitored by electron spin resonance gives an accurate measure of the complex dissociation characteristics that appear to parallel the helical coil cooperative melting transition monitored by absorbance measurements. The extent of dissociation of the bound spin labels parallels almost exactly the melting, i.e., unzipping of the DNA double helix, suggesting that the spin labels are immobilized by intercalation at the site on the DNA directly involved in the maintenance of the double helical structure. Analysis of the electron spin resonance spectra of the complexes in terms of the extent of immobilization indicates that, for aromatic amine spin labels, two different binding species exist, one strongly immobilized presumably at a guanine-cytosine base-pair region and the other partially immobilized in a region other than guanine-cytosine base pair. Competitive binding of the labels with respect to their parent ligand and binding isotherm results show that nitroxide substitution does not affect the type of binding but does affect the extent of binding, i.e.. binding constant with the labeled compound having a lower affinity.

Amines↗

PubMed1976

The effects of hydroquinone, hematin and heme-containing proteins on prostaglandin biosynthesis by methylcholanthrene-transformed mouse BALB/3T3 fibroblasts.

Cultured methylcholanthrene-transformed 3T3 mouse fibroblasts secrete large amounts of prostaglandin into the medium. Prostaglandin biosynthesis by these cells can be stimulated by arachidonic acid and, to a much lesser extent, by glutathione and porphyrin. In the presence of hydroquinone, prostaglandin production is increased; the most striking increases are seen at concentrations that are lethal. Porphyrins, when added to cells in the presence of lethal levels of hydroquinone, inhibit stimulation of prostaglandin E2 synthesis but stimulate the production of prostaglandin F2alpha.

Animals↗

PubMedDate not supplied

Activity-wheel running blunts suppression of splenic natural killer cell cytotoxicity after sympathectomy and footshock.

We used chemical sympathectomy by 6-hydroxydopamine (6-OHDA) to examine whether adaptation by the sympathetic nervous system (SNS) is a plausible explanation for our prior finding that activity-wheel running blunts the suppression of splenic natural killer cell cytotoxicity after footshock. Male Fischer rats were assigned to treatments using a group (activity wheel vs. sedentary)x treatment (6-OHDA vs. saline)x condition (footshock vs. no shock) design. After 5-6 weeks, rats were injected i.p. with saline or with 40, 80, and 80 mg/kg 6-OHDA on pre experimental days -5, -3, and -1. Half the rats received 6 min of random footshock during a 40-min period. Cytotoxicity was determined by standard 4-h 51Cr release assay. Sympathectomy reduced splenic [NE] by 72%. After 6-OHDA injection and footshock, percent lysis was 33% lower in sedentary rats compared with activity-wheel runners and home-cage controls, p=0.048. The results suggest that activity-wheel running leads to adaptations that offset an altered SNS modulation of splenic NK cell cytotoxicity in response to footshock.

Animals↗

PubMedDate not supplied

Cytokines and innate immunity of fish.

Explore the source record for details and available documents.

Animals↗

PubMedDate not supplied

Multinucleation of koilocytes is actually multilobation.

In order to clarify the complete cytomorphology of cytopathic changes as a consequence of human papillomavirus (HPV) infection, we performed three-dimensional (3D) reconstruction from confocal fluorescent images. After confirming 22 HPV types using a DNA chip, we performed 3D confocal image restoration in human uterine cervical swab samples and corresponding tumor tissues. On restoration of 3D confocal images, the multinucleated feature of koilocytes was revealed to be multilobation of a single nucleus, as opposed to true multinucleation.

Cell Nucleus↗

PubMedDate not supplied

Five restriction fragment length polymorphisms of the APOA1-C3 gene and their influence on lipids and apolipoproteins in healthy Chinese.

The distribution of five restriction fragment length polymorphisms (RFLPs) of the APOA1-C3 gene cluster and their influence on serum lipids and apolipoprotein levels was investigated in 151 healthy Chinese of both sexes. The frequencies of the rare alleles at ApaI, BanI, XmnI (A1) and SstI (C3) sites were significantly different in the Chinese when compared to Caucasians as follows: ApaI: 0.25 vs. 0.42 (p < 0.02); BanI: 0.33 vs. 0.16 (p < 0.01); XmnI: X2, 0.30 vs. 0.14, and X3, 0.08 vs 0.05 (p = 0.001); SstI (C3): 0.23 vs. 0.12 (p = 0.011). The frequency of P2 (PstI) at 0.04 was similar to that in Caucasians (0.07). The distribution of the genotypes of all the RFLPs was in Hardy-Weinberg equilibrium in this population. A significant association of the SstI polymorphism of the C3 region with the serum high-density lipoprotein (HDL)-cholesterol level was observed in both men and women, the rarer allele (S2) being associated with higher levels (p < 0.05). 5.8% of the sample variance of the HDL-cholesterol level in this sample could be explained by the SstI polymorphism of the C3 region (F = 6.07, p = 0.003). The association of the SstI locus with serum HDL-cholesterol was stronger in males than in females (R2 = 13.8 and 6.7%, respectively). There was a similar trend of association of the serum apolipoprotein A-I level with the SstI polymorphism, though it did not reach statistical significance. There was no association between the levels of any of the lipid and apolipoproteins studied with RFLPs of the APOA1 gene.

Adult↗

PubMedDate not supplied

Lipoxygenase inhibitors prevent lung carcinogenesis and inhibit non-small cell lung cancer growth.

The effects of lipoxygenase inhibitors were investigated using human lung cancer cell lines and A/J mice. By RT-PCR, 5-, 12-, and 15-lipoxygenase mRNA was detected in NSCLC cells. NDGA inhibited 5-LO activity in adenocarcinoma cell line NCI-H1264. Using an MTT assay, NDGA, MK591 and AA861 inhibited the growth of NSCLC cell lines tested with IC50 values of 3, 2, and 7 microM, respectively. Using a clonogenic assay, 10 microM NDGA significantly reduced NSCLC colony number. NDGA significantly slowed NSCLC xenograft growth in nude mice. When the tumors were excised and analyzed, nude mice treated with NDGA had significantly more apoptotic figures than did untreated tumors. A/J mice treated with urethane developed adenomas after 4 months and NDGA administration significantly reduced lung adenoma number. These data indicate that lipoxygenase inhibitors inhibit lung cancer growth and prevent lung carcinogenesis.

Adenocarcinoma↗

PubMedDate not supplied

Adoption of Korean children by New York area couples: a preliminary study.

This paper reports on the first segment of a longitudinal study of the effects of transracial and transcultural transplantation into American families.

Adoption↗