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Biomedical subjects

S Holland

Publications and source records attributed to S Holland.

126 records · Page 7Linked to original sources
PubMed1970

A microsurgical methodology for human cells in vitro: evolution and applications.

A methodology for micromanipulating human cells of normal and malignant origin, in vitro, has evolved from the study of about 2000 HeLa, ERK (a subline of HeLa cells), and human embryonic lung cells during interphase and mitosis. It is now possible to microinject interphase cells with aqueous and nonaqueous fluids intracytoplasmically. Chromosomes from human embryonic lung metaphase cells have been transplanted. Chromosomes have been manipulated within mitotic human embryonic lung, ERK, and HeLa cells. Clones have been obtained from HeLa cells subjected to such manipulation. Predictable derangements of mitotic cells and their progeny have been obtained. Intranuclear injections of silicone oil, DNA, and sodium chloride solutions have been made with survival of the cells. HeLa cells have been cloned from such injected cells. Subcellular fractions have been introduced into the nuclei and cytoplasms of HeLa and human embryonic lung cells. The lung cells have been subjected to nuclear micropuncture in groups and a clone has been obtained. Virus suspensions have been introduced into the nuclei of HeLa cells without killing the cells. Applications of this methodology are discussed.

Cell Line↗
PubMedDate not supplied

Diagnosing non-gonococcal urethritis: the gram-stained urethral smear in perspective.

The value of microscopy of a Gram-stained urethral smear in the diagnosis of non-gonococcal urethritis and chlamydial urethral infection was assessed in 153 men attending a department of genitourinary medicine. A mean of more than 4 polymorphs in 5 fields (x1000) was considered to be abnormal. In the diagnosis of urethritis, the sensitivity (94%), specificity (91%), positive predictive value (93%) and negative predictive value (93%) were high. In the diagnosis of chlamydial infection, sensitivity (91%) and negative predictive value (96%) were high, but specificity (68%) and positive predictive value (46%) were low. In men with symptoms of genitourinary infection, the sensitivity (96.7%) and negative predictive value (97.4%) of the Gram-stained urethral smear in the diagnosis of chlamydial infection was comparable with modern chlamydial antigen detection tests.

Chlamydia Infections↗
PubMedDate not supplied

Approaching with care: violence at work (continuing education credit).

Violence against nurses, midwives and health visitors is much more common than is generally supposed or admitted. With care, by using appropriate strategies, violent and aggressive incidents can be prevented or managed, to minimise harm to all concerned. Staff support during and following an incident is vital.

Aggression↗