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Biomedical subjects

S Hirose

Publications and source records attributed to S Hirose.

At least 757 records · Page 42Linked to original sources
PubMed1984

Biochemical and immunohistochemical localization of renin in human pituitary.

The renin content of human pituitaries obtained at autopsy of eight patients (four males and four females) within 3 hours after death from various diseases was determined. The pituitaries contained a significant amount of renin (5.5 +/- 1.6 mg of angiotensin I/mg of protein/hour). Although much less (1/1000) than that in human kidneys (4.5 micrograms of angiotensin I/mg of protein/hour), this content was about 10 times higher than that in normal plasma (0.5 ng of angiotensin I/mg of protein/hour). More than 50% of the renin activity was inhibited by antihuman renin antibody. These results are the first detection of a significant amount of renin in the human pituitary. No difference in pituitary renin content between males and females was detected; the pituitaries from the two youngest patients showed the highest levels. Immunohistochemical staining showed no positive cells in the intermediate and posterior lobes and many positive cells in the anterior lobe. Serial sections were examined, and renin-containing cells were immunohistochemically identified as polactin-producing cells without distinction as to the sex of the donor. This demonstration of the intracellular presence of immunoreactive renin lends strong support to the idea that renin is not a contaminant of pituitary preparations due to entrapped plasma, but is endogenous to the human pituitary.

Adolescent↗
PubMed1984

A new sensitive direct radioimmunoassay for human plasma renin and its clinical application.

We developed new sensitive direct radioimmunoassay for human plasma renin. Renin was purified from Haas' preparation utilizing a pepstatin-C6-Sepharose affinity chromatography. Antiserum, prepared by immunizing rabbits with the purified renin, was used for the direct radioimmunoassay at a final dilution of 1:30,000. The antibody was specific for human renal and plasma renin, but did not cross-react with cathepsin D, trypsin, or renins of mouse, dog, and rat. Radioimmunoassay was performed by the double antibody technique using the delayed tracer addition method. In this method, a standard curve was obtained over a range from 0.2 to 8.0 ng/ml. The values from our assay correlated well with total renin activity measured as the generation rate of angiotensin I after trypsin activation (r = 0.78, p less than 0.01), but correlated weakly with active renin activity. This finding disclosed that both active and inactive renin were detected by this method. In normal participants, plasma renin concentration determined by direct radioimmunoassay was increased by standing and furosemide injection. The plasma renin concentration determined by direct radioimmunoassay of patients with essential hypertension (0.7 to 1.7 ng/ml) was not significantly different from values in normal controls (0.8 to 1.9 ng/ml). The values were higher in patients with renovascular hypertension (1.6 to 2.7 ng/ml), malignant hypertension (2.8 to 3.4 ng/ml) and Bartter's syndrome (1.8 to 2.5 ng/ml), but lower in patients with primary aldosteronism (0.4 to 0.8 ng/ml) than in normal controls. This newly developed radioimmunoassay for human renin was sensitive enough to estimate the levels of renin in plasma of patients with low renin hypertension. It provides a new tool for the understanding of the renin-angiotensin system under various clinical conditions.

Adult↗
PubMed1984

[Adjuvant chemotherapy after surgery in advanced gastric cancer].

Two hundred and fifty-four patients with advanced gastric cancer underwent radical surgery and ftorafur (FT) or 5-fluorouracil (5-FU) was orally administered as the adjuvant chemotherapy (ACT). Recurrence after ACT were analyzed by the quantification method II. As a result, it was found that: 1) ACT over 2 years could lower the recurrence rate, and careful follow-up is still necessary up to 4 years, and 2) after the remission induction therapy with mitomycin C and/or 5-FU and cytarabine, 12 mg/kg/day of FT for the first year and 8 mg for the second year are advisable.

Administration, Oral↗
PubMed1984

Fluorescence labelling of pyrimidine nucleobases and their related compounds for high-performance liquid chromatography.

Fluorescence derivatization of pyrimidine nucleobases and nucleosides with 4-bromomethyl-7-methoxycoumarin(Br-Mmc) was studied. In dimethylsulfoxide(DMSO) containing powdered potassium carbonate, the reaction completed within 5 min at room temperature. The excess Br-Mmc was treated with p-nitrobenzoic acid(NBA) which gave a non-fluorescent compound. For the determination of serum sample, diethylaminoethyl sepharose(DEAE) column was used in a cleanup procedure.

Chromatography, High Pressure Liquid↗
PubMed1984

[Diaphragm pacemaker implantation: a case report of central hypoventilation due to cerebellar bleeding].

A 60-year-old male developed chronic respiratory failure due to central hypoventilation after cerebellar bleeding. We implanted a diaphragm pacemaker (radio-frequency type) to the patient, and his respiratory status was improved. Although this apparatus devised by Glenn has been widely used in the United States, clinical applications in Japan were rarely reported. Diaphragm pacing might be one of the effective treatments to the patient with chronic respiratory failure. We discussed the indication, mechanical subject and surgical approach concerning diaphragm pacemaker implantation in this paper.

Cerebellar Diseases↗
PubMed1984

Prophylaxis of upper gastrointestinal bleeding with cimetidine in patients undergoing partial hepatectomy.

The aim of this study was to evaluate the effect of cimetidine on prophylaxis of upper gastrointestinal (GI) bleeding from acute mucosal lesions or stress ulcers of the stomach and duodenum after partial hepatectomy. Five (27.8%) of 18 control patients had upper GI bleeding v.s. only two (5.9%) of 34 cimetidine treated patients (P less than 0.05). The result showed that cimetidine therapy is an effective modality to prevent upper GI bleeding after liver resection but there was a trend that it may induce postoperative liver failure and hepatitis. A further study is necessary to elucidate whether cimetidine therapy is really safe following major hepatic resection.

Adult↗
PubMed1983

Fluorometric determination of hypoxanthine and xanthine in biological fluids by high-performance liquid chromatography using enzyme reactors.

A selective and sensitive assay of hypoxanthine and xanthine in biological fluids by high-performance liquid chromatography coupled with immobilized-enzyme reactors was developed. The separations were achieved by reversed-phase liquid chromatography. Hydrogen peroxide produced from hypoxanthine and xanthine by immobilized xanthine oxidase was determined fluorometrically using immobilized peroxidase and p-hydroxyphenylacetic acid. Immobilized enzymes were prepared by intermolecular cross-linking to controlled-pore glass. Assay of allopurinol was also possible by the present method. The method was applied to serum and urine. The detection limits of hypoxanthine and xanthine were approximately 50 and 120 pg per injection, respectively.

Chromatography, High Pressure Liquid↗
PubMed1983

Differential stimulation by polyamines of phage DNA-directed in vitro synthesis of proteins.

The effect of polyamines on T7- and lambda rifd18 DNA-directed synthesis of proteins in an Escherichia coli cell-free system has been studied. When T7 DNA was used as a template, the degree of stimulation by spermidine of protein synthesis was larger with T7 RNA polymerase than with Mr 42 K protein, while the synthesis of Mr 13.5 K protein was not stimulated significantly by spermidine. The synthesis of T7 RNA polymerase was stimulated approx. 10-fold by 1 mM spermidine. When lambda rifd18 DNA was used as a template, the synthesis of beta beta' subunits of RNA polymerase was stimulated greatly by spermidine, while the synthesis of elongation factor Tu and ribosomal proteins was not stimulated significantly by spermidine. Spermidine stimulation of T7 DNA-directed synthesis of T7 RNA polymerase was at the level of both translation and transcription. The degree of stimulation by spermidine was greater at the level of translation. Putrescine stimulated the synthesis of T7 RNA polymerase and Mr 42 K protein to a small degree at the level of translation.

Bacteriophage lambda↗
PubMed1983

Enhancing effect of H-2-linked NZW gene(s) on the autoimmune traits of (NZB X NZW)F1 mice.

To investigate the possible enhancing effect of the H-2z haplotype of the New Zealand White (NZW) strain on the production of autoantibodies and renal disease observed in B/W F1 mice, we developed the ZWD/8 strain, a NZW congenic line carrying the H-2d haplotype, produced (NZB X ZWD/8)F1 (B/WD8 F1) mice, and examined the difference in several immunological abnormalities between the B/W F1 (H-2d/H-2z) and the B/WD8 F1 (H-2d/H-2d) mice. In comparison with B/W F1 mice, the B/WD8 F1 mice showed markedly lower serum levels of the anti-DNA antibodies and the gp70 ICs, and a later onset and a lower incidence of proteinuria with a lower mortality. In contrast, there was no significant difference in the incidences and the amounts of both natural thymocytotoxic autoantibody and anti-erythrocyte autoantibody between these two hybrid strains. Further, the serum levels of IgG and IgM in B/WD8 F1 mice were as high as those in B/W F1 mice. These findings indicate that the gene(s) that is within or closely linked to the H-2 complex of NZW strain specifically acts to intensify the levels of anti-DNA antibodies and gp70 ICs, and to promote the severity of renal disease in B/W F1 mice. This gene may play a role in the class conversion of anti-dsDNA antibodies from IgM to IgG.

Animals↗
PubMed1983

Polyamine inhibition of gastric ulceration and secretion in rats.

The effect of polyamines on gastric ulceration and secretion in rats was studied. Stress-induced gastric ulceration and ulceration in pylorus-ligated rats were inhibited by subcutaneous or oral administration of spermine; spermidine's inhibitory effect was somewhat less. Histamine-induced ulceration was also inhibited by the subcutaneous injection of spermine. In addition, the daily oral administration of spermine for 10 days was therapeutic against an acetic acid-induced ulcer (chronic ulcer). Gastric secretion in pylorus-ligated rats and in rats with fistulae and stimulated by histamine injection was decreased by the subcutaneous injection of spermine.

Animals↗
PubMed1983

A novel purification method of human renin.

Human renal renin was isolated from a partially purified preparation, Haas' preparation step 5 material, with a remarkably high yield of 28% by a newly developed method. This method consisted of only four steps including affinity chromatography on pepstatin-aminohexyl-agarose, chromatofocusing, gel filtration and DEAE-chromatography after the initial extraction and concentration. This method enabled us to obtain pure human renin without another affinity column used by other investigators. Pure human renin had a molecular weight of 40,000 daltons as estimated by gel filtration and sodiumdodecylsulfate-polyacrylamide gel electrophoresis. The pI of purified renin was 5.6.

Chromatography, Affinity↗
PubMed1983

Effect of polyamines on synthesis and degradation of guanosine 5'-diphosphate 3'-diphosphate.

The effect of polyamines on the in vitro and in vivo synthesis and degradation on guanosine 5'-diphosphate 3'-diphosphate (ppGpp) has been studied in Escherichia coli. The presence of 2 mM spermidine lowered the optimal Mg2+ concentration for ppGpp formation from 17 mM to 11 mM. The formation of ppGpp in the presence of 2 mM spermidine and 11 mM Mg2+ was about 15% greater than that in the presence of 17 mM Mg2+. At a concentration of less than 11 mM Mg2+, spermidine was found to stimulate ppGpp formation greatly. Putrescine did not cause any effect. When a polyamine-requiring mutant of E. coli (EWH319) was starved for an amino acid by the addition of valine, spermidine stimulated ppGpp formation. The degradation of ppGpp was not influenced significantly by polyamines.

Bacterial Proteins↗