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Biomedical subjects

S Higashi

Publications and source records attributed to S Higashi.

At least 271 records · Page 15Linked to original sources
PubMed1980

Effect of ACTH on adrenal estrogens.

Tissue levels of rat adrenal estrogens during the estrous cycle were determined by radioimmunoassay, and effect of ACTH on the adrenal estrogen levels was examined. The levels of estrone and estradiol in proestrus were 2.75 +/- 0.61 pg/mg tissue and 1.0 +/- 0.19 pg/mg tissue (mean +/- SE), respectively. The higher level of estrone but not of estradiol was inclined to appear in proestrus rather than in other phases. Injection of 250 IU/kg of alpha 1-24-ACTH significantly increased the estrone level at 15 and 30 min, and estradiol at 15 min. Administration of reserpine, which was used as the ACTH releaser, produced an increase in the estrone level but did not change the estradiol level. While dexamethasone injection decreased the estrone level significantly at 8 hours after the treatment, the level of estradiol remained unchanged. Hypophysectomy produced a considerable decrease in both steroid levels, particularly estrone. These results indicate that the major estrogen in the rat adrenal gland is estrone and that the level is regulated by hypophyseal ACTH.

Adrenal Glands↗
PubMed1980

Adrenal estrogens in relation to ovarian function.

The effect of gonadotropins and ovarian hormones on adrenal estrogens in female rats was examined in relation to the ovarian function. Pregnant mare serum gonadotropin (PMS) increased adrenal estradiol level in intact rats but did not alter estrone levels. Human chorionic gonadotropin (HCG) with PMS did not alter the estrone and estradiol levels. Neither PMS or HCG with PMS affected these steroid levels in ovariectomized rats. Injection of hexestrol to intact rats decreased the estrone level. Further decrease in the estrone level resulted after administration of hexestrol with progesterone. In a developmental study, the estrone level at 35 days of age was higher than that at 50 and 80 days of age. The estrone level gradually decreased with adrenal development after ovariectomy performed at 21 days of age. When adrenal slices were incubated with either 3H-estrone or 3H-estradiol, higher radioactivity of estrone accumulated in the adrenal gland of immature rats than in that of adult rats. There was a larger conversion of 3H-estradiol to 3H-estrone than the reverse process in both immature and adult rats. These results indicate that the adrenal estrogens were influenced by ovarian function.

Adrenal Glands↗
PubMed1980

[Influence of methyl 0-(4-hydroxy-3-methoxy-cinnamoyl) reserpate (CD-3400) on the gonadal and adrenal functions in rat (author's transl)].

Influence of CD-3400, which has been developed as a new antihypertensive agent in the class of rauwolfia alkaloids, was studied and effects were compared to those seen with reserpine. A single, oral administration of CD-3400 in a dose of 50 mg/kg to female rats had no effects on ovarian estradiol levels in contrast to the significant decrease at 6 and 18 hr seen after reserpine administration. CD-3400 given in a dose of 50 mg/kg to female rats increased to a lesser degree the adrenal corticosterone level at 1 hour than did reserpine. While the serum corticosterone level in the female rats was not changed with CD-3400 administration, a large increase was detected in the case of reserpine. Administration of CD-3400 or reserpine in a dose of 100 mg/kg to male rats produced no change in the testicular testosterone level, but in both cases induced a significant decrease in the testicular estradiol levels. This inhibition was higher in the case of reserpine treatment. In addition, the serum corticosterone level in male rats treated with reserpine was higher than levels after administration of CD-3400. Our results indicate that CD-3400 exerts a weaker effect than does reserpine on gonadal and adrenal functions of both sexes of rats.

Adrenal Glands↗
PubMed1979

Conversion of 7-ketolithocholic acid to ursodeoxycholic acid by human intestinal anaerobic microorganisms: interchangeability of chenodeoxycholic acid and ursodeoxycholic acid.

Chenodeoxycholic acid, ursodeoxycholic acid and 7-ketolithocholic acid were incubated with human intestinal bacteria (source: 4 healthy males) at 37 degrees C for 72 hours in an anerobic condition. The bile acids of the products in culture medium were identified by three independent methods, thin layer chromatography, gas-liquid chromatography and GLC-mass spectrometry. Lithocholic acid, ursodeoxycholic acid and 7-ketolithocholic acid were observed in the culture of chenodeoxycholic acid. Lithocholic acid, chenodeoxycholic acid and 7-ketolithocholic acid were observed in the culture of ursodeoxycholic acid. Chenodeoxycholic acid and ursodeoxycholic acid were produced from 7-ketolithocholic acid. These data may suggest that chenodeoxycholic acid and ursodeoxycholic acid are interconvertible via 7-ketolithocholic acid by the mixed culture of human intestinal microorganisms under an anaerobic condition.

Anaerobiosis↗