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Biomedical subjects

S Higaki

Publications and source records attributed to S Higaki.

At least 73 records · Page 4Linked to original sources
PubMed1987

In vitro calcification in human osteoblastic cell line derived from periosteum.

Human osteoblastic cell cultures were established from human periosteum, identified on the basis of high alkaline phosphatase activity in the confluent state. The cells in the growing state showed a fibroblastic morphology and grew to form multiple layers. The cells could spontaneously calcify, and the process was accelerated by alpha-glycerophosphate. Minerals deposited on the cells consisted exclusively of calcium and phosphorus, and matured into hydroxyapatite crystals. The calcification was stimulated by the treatment with 1 alpha,25-dihydroxy-vitamin D3. These results indicate that the osteoblastic cells have the capacity to differentiate into osteocytes and form calcified human bone tissue in vitro.

Adult↗
PubMed1987

[Limb salvage in osteosarcoma. Significance of preoperative isolation perfusion chemotherapy].

In our Department since 1960 isolation perfusion chemotherapy has been performed as a preoperative procedure combined with surgery in 134 patients with osteosarcoma of the extremity. In this paper, the significance of isolation perfusion chemotherapy was presented. The cumulative 5-year survival rate of 114 perfused patients was 42.7%, while that of 41 non-perfused patients before 1960 was 17.1%. Of the 114 patients 102 underwent amputation and 12 had limb salvage surgery. The cumulative 5-year survival rate of the 9 patients who have received limb salvage surgery since 1978 is 68%. Eleven patients who received preoperative aggressive chemotherapy including isolation perfusion chemotherapy showed a 91% clinical and radiological response to the drug. The tumor was microscopically analyzed and the ratio of necrotic to viable tumor cells was calculated. The degree of tumor necrosis was classified as excellent (greater than or equal to 95%), good (80-94%), fair (50-79%) and poor (less than 50%). The response in 22 patients who received preoperative aggressive chemotherapy using adriamycin, methotrexate and cis-platinum including the use of isolation perfusion was excellent in 41%, good in 36%, fair in 14% and poor in 9%. We believe that preoperative isolation perfusion chemotherapy is an effective procedure for limb salvage surgery.

Adolescent↗
PubMed1984

Type of collagen in chordoma.

Pepsin-soluble collagen was extracted from three histologically proven cases of chordoma and nucleus pulposus. The collagen types of these materials were investigated by differential salting-out, SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) of native collagen and their CNBr (cyanogen bromide) cleaved peptides, and their amino acid compositions. Although the collagen of nucleus pulposus was type II, that of chordoma was largely type I. Collagen of notochord, the origin of nucleus pulposus, is known to be type II. Further investigation is necessary in view of the fact that collagen of chordoma, a tumor believed to be derived from notochord, is not type II.

Aged↗
PubMed1983

[Clinical features and treatment of pulmonary metastasis in osteosarcoma].

Ninety-seven pulmonary metastatic cases in 141 osteosarcoma from 1960 to 1981 were analysed. The overall cumulative 5-year survival rate of the cases was 38.1%, whereas that of the recent 41 cases with systemic chemotherapy was 56.2%. Eighty percent of pulmonary metastases were found within one year since their first visit to our clinic. It was revealed that there were positive relationship between the doubling time of the pulmonary metastases and the post-metastatic survival (p less than 0.001), and between the period from their first visit to our clinic to metastases and the post-metastatic survival (p less than 0.001). The favorable effects of the systemic chemotherapy using adriamycin, vincristine, methotrexate and cis-platinum were recognized. The thoracotomy revealed effective and five cases are living without disease for average 80.8 months after surgery.

Antineoplastic Agents↗
PubMed1983

[Determination of malignancy of cartilaginous tumors (2). Determination of malignancy by nuclear DNA contents and their distribution patterns in cartilaginous tumors].

In 40 different types of cartilaginous tumors, the number of binuclear cells was calculated and DNA content was examined using fluorescent Feulgen cytophotometric technique. In 7 enchondromas and 1 synovial chondromatosis, the DNA content was diploid in 7 and hypotetraploid in 1, and showed a unimodal normal distribution. The number of binuclear cells was 0.1-0.45%, and the mean DNA content was below 4. These cases showed no evidence of disease after curettage and bone graft. The follow-up period was from 4 years 6 months to 7 years 1 month, averaging 6 years 2 months. In 2 enchondromatoses and 2 benign chondroblastomas, the DNA content was diploid and showed a unimodal normal distribution. One chondromyxoid fibroma showed a broad-unimodal tetraploid distribution, and one benign chondroblastoma showed a broad-aneuploid distribution. The nuclear DNA content was sometimes histologically similar to that of chondrosarcoma in some benign cartilaginous tumors. In 5 secondary chondrosarcomas, the DNA content was hypo- or hypertetraploid, hexaploid, and showed broad-unimodal or broad-bimodal distribution. The number of binuclear cells was from 13% to 23%. Although the size of the nucleus was small, the number of binuclear cells was evidently numerous. The preferred treatment for secondary chondrosarcoma is principally wide resection, but amputation is sometimes indicated in cases of recurrence and large tumor size. The follow-up period was from 2 years 2 months to 18 years 11 months, averaging 9 years 11 months. All 5 patients were surviving at the time of follow-up. The DNA content in chondrosarcoma was mostly hypo- or hypertetraploid, hexaploid, or octaploid and showed broad-unimodal, broad-bimodal or broad-aneuploid distribution. The number of binuclear cells was 0.55-3.5%. The mean DNA content was proportional to the number of binuclear cells, the positive correlation between the mean DNA content and the number of binuclear cells was found (p less than 1%).

Adolescent↗
PubMed1981

Crystalline inculsion in myxoid liposarcoma.

An electron-microscopic study of a myxoid liposarcoma is presented in which amorphous electron-dense material and crystalline inclusions in the granular endoplasmic reticulum were observed. These inclusions showed transversal striations and were associated with round electrodense bodies. The previous finding of similar structures in normal cells from vertebrates and plants without relationship to adipose tissue discards the direct association of these inclusions with this particular tumor and suggests a possible functional origin.

Aged↗
PubMed1981

[Clinicopathological study of twenty-two cases of benign chondroblastoma (author's transl)].

Benign chondroblastoma, a rare bone tumor clinically and histologically resembles giant cell tumor. We have experienced twenty-two cases of histologically confirmed benign chondroblastoma over the 25 year period from 1952 to 1978. The age of the patients ranged from 10 to 46 years, with an average of 20 years. Fifteen cases were in the second decade. Males were predominant to females with a ratio of 2.7:1. Location of the tumor varied widely. The tumor was located in the long bones in 18 out of 22 and limited to the epiphysis in 15. Detailed histological examination in twelve cases with hematoxylin-eosin, alucian blue, toluidine blue, silver impregnation and safranin-O staining, revealed positive staining with alucian blue and PAS. PAS positive granules wee found within cytoplasm and identified as glycogen granules by electron microscopy. However, metachromasia was not always positive and none of these cases stained positive with safranin-O. Rich reticulin fibers encircled each tumor cell and each group of cells. Electronmicroscopic study of 4 cases revealed two types of giant cells. The cell membrane had many microvilli-like protrusions, and the nuclei of the tumor cells were irregular in appearance and had large indentations. Sometimes many fine filaments and electron dense fine granules were observed in intercellular chondroid space. Morphological study of cultured cells was done in two cases. Morphology of these tumor cells proved to be different from fibroblasts, cultured chondrocytes and macrophages. Tumor cells moved rapidly and had undulating membranes at the pseudopodia. From these findings, we speculated that the origin of the benign chondroblastoma cell may be histiocytic cells. Surgical treatment of this tumor was carried out as follows. Curettage was performed in 16 cases, with bone graft in 15, resection 4 and amputation in two. Recurrence was noted on two cases, although no further recurrence occurred after reoperations for nine and twelve years respectively.

Adolescent↗