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Biomedical subjects

S Hidaka

Publications and source records attributed to S Hidaka.

At least 163 records · Page 9Linked to original sources
PubMed1986

The synaptic ultrastructure in the outer plexiform layer of the catfish retina: a three-dimensional study with HVEM and conventional EM of Golgi-impregnated bipolar and horizontal cells.

Synaptic structures between receptors and horizontal and bipolar cells in the outer plexiform layer (OPL) of Golgi-impregnated catfish retina were examined by conventional electron microscopy of serial ultrathin sections and by high-voltage electron microscopy (HVEM) of thick sections. Cone terminals contained multiple synaptic ribbons and rod terminals contained single synaptic ribbons. This observation was used to identify these two types of photoreceptors. The cone horizontal cell, located in the most distal part of the inner nuclear layer (INL), invaginated only cone terminals, whereas the rod horizontal cell, located in the proximal part of the INL, invaginated only rod terminals. Both lateral elements of the triad in the rod terminal originated from a single rod horizontal cell whereas the same structures in the cone terminal were often derived from several cone horizontal cells. Golgi-impregnated catfish bipolar cells were classified into two types based on the differences in their axonal arborization as described by Famiglietti et al. ('77). Axonal endings of type a bipolar cells were located in the distal part, sublamina a, of the inner plexiform layer (IPL), and axonal endings of type b cells were located in the proximal part, sublamina b, of the IPL. Dendrites from type a bipolar cells made direct contact with the synaptic ribbons in both rod and cone terminals whereas those from type b cells made indirect contact with the ribbons in both rod and cone terminals, but rare direct contact with the ribbon in rod terminals were also seen. In addition, bipolar cells made basal junctions or superficial contacts in both rod and cone terminals. The "lateral" processes of bipolar cells invaginating rods penetrated between the rod terminal and rod horizontal cell processes, and made basal junctions with both rod terminals and rod horizontal cells. There was no definitive morphological feature that could be associated with sign-conserving and sign-inverting signal transmission.

Animals↗
PubMed1985

Sequence analysis of a KpnI family member near the 3' end of human beta-globin gene.

We determined the complete nucleotide sequence (6125 bp) of a full-length member of human KpnI family, designated T beta G41, which is located about 3 kb downstream from the beta-globin gene. Comparison of the sequence with the KpnI family sequence compiled by Singer revealed that a new 131 bp sequence is present in the T beta G41. Hybridization analyses showed that a few thousand of human KpnI family members are carrying this additional sequence. Computer search of DNA databases for T beta G41-homologous sequence showed that some T beta G41-homologous sequences were closely associated with pseudogenes. The T beta G41 sequence also showed significant sequence homology with ChBlym-1, a transferrin-like transforming gene of chicken. Furthermore, an amino acid sequence deduced from the T beta G41 nucleotide sequence revealed a relatively-high homology to those of human transferrin and lactotransferrin.

Base Composition↗
PubMed1985

Structure and genomic organization of the rat aldolase B gene.

The structure of the chromosomal gene encoding rat aldolase isozyme B has been elucidated by sequence analysis of cloned genomic DNA. This gene comprises about 14 X 10(3) base-pairs of DNA, and is separated into nine exons by eight intervening sequences. A presumed transcription-initiation site was assigned by S1 nuclease protection mapping, and T-A-T-A and C-C-A-A-T boxes were found to be 25 and 126 base-pairs, respectively, upstream from this initiation site. There are three characteristic sequences of 100 to 200 base-pairs within the region of 870 base-pairs flanking the 5' side of the gene. These sequences are flanked on either side by direct repeats and terminate with an A-rich stretch of nucleotides. One of them has block homology with a region in an "ID sequence", which is reported to be an element for tissue-specific gene regulation and differentiation. The other two are analogous at the sequence organizational level with a sort of dispersed repeat, the "Alu family". These features suggest that these regions are involved in gene regulation and, also, imply evolutionary events such as duplication or insertion. Comparison of this gene sequence with the rabbit aldolase A complementary DNA sequence revealed some bias in the frequency of nucleotide replacement among the exons, suggesting selective evolutionary conservation of particular exons encoding functional domains. Comparison with the human aldolase B complementary DNA sequence revealed no such tendency; the homology between the two sequences was very high (about 89%), and nucleotide replacements were randomly distributed throughout the protein-coding region.

Animals↗
PubMed1985

Messenger RNA structure participating in the initiation of synthesis of cucumber mosaic virus coat protein.

The sequence of the 5'-terminal 106 nucleotides of cucumber mosaic virus (strain Y) RNA 4, the mRNA coding for viral coat protein, has been determined. The first AUG was located at 77 nucleotides from the 5'-terminus and was confirmed to be an initiation codon by analysis of the N-terminal amino acid sequence of the protein. The nucleotide sequence (positions 77-106) beyond the AUG codon predicted the sequence of ten amino acids corresponding to the N-terminal region of the protein, which exactly matched the determined amino acid sequence containing an acetyl methionine as the N-terminal amino acid. The distance of the initiation codon AUG from the cap structure was 76 nucleotides and the longest among the mRNAs for coat protein of plant viruses so far reported (9-36 nucleotides). This noncoding region is rich in U residues (40%) and the number of G residues (21 nucleotides) is the largest among these mRNAs (usually 1 or 2 residues). A possible secondary structure is postulated for the region, which might be implicated in efficient translation of the RNA 4 in vivo.

Amino Acid Sequence↗
PubMed1985

Interleukin 2 acts directly on a Tac-positive cloned B cell line established from a patient with adult T cell leukemia.

A Tac-positive B cell line termed K3B was established from a patient with adult T cell leukemia (ATL). This cell line had EBNA antigen and human T cell leukemic virus (HTLV) provirus besides B1 antigen and surface immunoglobulin. A cloned Tac-positive B cell line termed K3B01 was obtained from K3B by the limiting dilution method. The K3B01 cells were shown to absorb IL 2 activity in a tonsillar IL 2 preparation. By using this cloned cell line and a purified recombinant IL 2 preparation, it was shown that the proliferation of K3B01 cells was enhanced by the addition of recombinant IL 2. Moreover, this response was inhibited by anti-Tac antibody. These results demonstrate definitively that IL 2 acts directly on B cells through IL 2 receptors on them.

Adult↗
PubMed1985

[An experimental study on pyogenic osteomyelitis with special reference to polymicrobial infections].

The author used the following method to successfully devise a model of experimental osteomyelitis caused by two different bacteria. A 3-mm silk thread (No. 5) soaked in Staphylococcus aureus (1.0 X 10(4) CFU) and Pseudomonas aeruginosa (1.0 X 10(5) CFU) suspensions was dried under reduced pressure. The silk thread was inserted into the metaphysis of the right tibia of mice. Experimental osteomyelitis caused by these two species of bacteria was successfully produced in all the mice, and this infection was similar to osteomyelitis in man. After prior infection with S. aureus, P. aeruginosa (1.0 X 10(3) CFU) was inoculated directly into the tibia of mice during the acute and chronic stages of the S. aureus infection, and secondary infection by P. aeruginosa was confirmed in all the mice. Using this experimental model of osteomyelitis, the author administered an antibiotic that is effective against only S. aureus in mice and observed abrupt growth of P. aeruginosa to elucidate certain aspects of the bacterial replacement phenomena.

Animals↗
PubMed1984

Binding sites of rat liver 5S RNA to ribosomal protein L5.

The ribonucleoprotein complex consisting of 5S RNA and the protein L5 was prepared from the large subunit of rat liver ribosomes. The RNA in the complex was digested in situ with RNase A or RNase T1. The RNase-resistant RNA fragments bound to the protein were recovered and purified by 2D-PAGE, and their nucleotide sequences were determined in order to elucidate the binding sites of the RNA to the protein. The results showed that the fragments had arisen from the 5'-end region (residues 1-21), from the second hairpin loop (residues 77-102) and from the 3'-end region (residues 106-120). Harsher digestion trimmed these fragments to shorter fragments. It was concluded that the minimal interactive sequences of 5S RNA to the protein L5 were residues 13-21, residues 85-102, and residues 106-114. A part of the first hairpin loop, residues 41-52, was also suspected to interact with the protein. These protein-binding sites of rat liver 5S RNA were compared with those of Escherichia coli, Halobacterium cutirubrum and yeast, and their probable conservation from eubacteria to eukaryotes is discussed.

Animals↗
PubMed1984

Refracture of bones of the forearm after plate removal.

Thirty-two plates originally used for fracture fixation in the ulna and radius in twenty-three patients were removed at Hennepin County Medical Center in Minneapolis between 1977 and 1982. The plate was on the ulna in eighteen arms and on the radius in fourteen. Removal of twenty-one plates was elective, and eleven were removed because of slight pain or discomfort. The interval between plate application and plate removal ranged from eight to sixty-two months. The average duration of cast immobilization used for protection after removal of the plate was six weeks. There were seven refractures, which occurred between two and forty weeks after plate removal. Three refractures occurred at the former fracture site; three, through the fracture site, extending into an adjacent screw-hole; and one, at one screw-hole. No refracture occurred more than forty weeks after removal of the plate.

Adolescent↗
PubMed1983

Rat aldolase isozyme gene.

Rat aldolase B mRNA was partially purified from liver polysomes by an immunochemical technique followed by oligo(dT)-cellulose column chromatography. Double-stranded cDNA, synthesized from this mRNA, was inserted into the PstI site of plasmid pBR322 employing the oligo(dC)-oligo(dG) tailing method. Clones containing aldolase B cDNA inserts were selected by colony hybridization using 32P-labeled purified mRNA as a specific probe. Several recombinant plasmids containing 600 to 1000 base pair inserts were isolated. Hybrid selection-translation experiments showed that they hybridize specifically with aldolase B mRNA. By overlapping restriction maps of several individual cDNA inserts, it was found that they spanned 1200 base pairs, which represented about 70% of the aldolase B mRNA sequence. The nucleotide sequence of the cDNA was then determined and the sequence of 180 amino acids from the COOH terminus and the entire 3' untranslatable nucleotide sequence were clarified. Although the complete amino acid sequence of rat aldolase B has not yet been reported, it was found that several amino acids neighboring the COOH-terminal tyrosine obtained by carboxypeptidase digestion completely coincided with those determined from the cDNA sequence; i.e. -Ser-Leu-Phe-Thr-Ala-Ser-Tyr-Thr-Tyr. Furthermore, a putative active site peptide appeared and is extensively homologous to those of rabbit aldolases A and B.

Amino Acid Sequence↗
PubMed1983

Regional difference in density of monoamine-accumulating cells of carp and catfish retinas.

By means of a histofluorescence technique, a comparative study was conducted on the regional density of dopaminergic (DA) and indoleamine-accumulating (IA) cells in carp (Cyprinus carpio) and catfish (Ictalurus punctatus) retinas. In order to enhance detection of fluorescent cells, noradrenaline (NA; 5.0 micrograms) or a mixture of NA (2.5 micrograms) and 5,6-dihydroxytryptamine (5,6-DHT; 2.5 micrograms) was intravitreally injected into the eyes 2-3 hr before enucleation. DA and IA cells were counted systematically in space on flat-mounted preparations. Both classes of cells were found to be distributed similarly in the two species of fish; the cell density is highest in the circumferential margin of the retina, and is slightly higher in a region dorsal to the optic disc than in the surrounding area. Differences in the distribution pattern of the cells between carp and catfish retinas were as follows: (a) the DA cell density is higher over the whole retinal field in carp (the mean density +/- SD = 34 +/- 16 cells/mm2) than in catfish (13 +/- 7 cells/mm2); (b) the region where the density is slightly higher than in the surrounding area is restricted to a small area immediately dorsolateral to the optic disc in carp, while it is relatively broadly placed dorsal to the optic discs, forming a horizontal band in catfish; (c) the density ratio of DA cells to IA cells is 1:1 in carp but 1:2 in catfish; and (d) catfish DA cells seem to be more irregular than carp DA cells in shape, size, dendritic arborization, uptake preference for monoamines intravitreally injected, and also in depth location seen in radial cryosections.

Animals↗
PubMed1983

Pathogenesis and treatment of osteochondritis dissecans--an experimental study on chondral and osteochondral fractures in adult and young rabbits.

To observe the natural history of chondral injury and clarify the genesis of osteochondritis dissecans, various types of experimental osteochondral or chondral injuries were made on the articular surface of femoral condyles of adult and young rabbits. Pathological features resembling osteochondritis dissecans were frequently observed in young rabbits, especially in the groups in which the injured part was most unstable (58% in rabbits aged from 7 to 14 days, 24% in those aged from 3 to 7 weeks). Therefore, a neglected chondral injury in early childhood and following ossification disturbances may relate to this disease.

Animals↗
PubMed1982

Relationship between structure of the 5' noncoding region of viral mRNA and efficiency in the initiation step of protein synthesis in a eukaryotic system.

To determine whether the rate of protein synthesis is controlled by the structure of mRNA near its 5' terminus, protein-synthesizing ability, especially in its initial stage, was compared among RNAs of plant viruses. Those viruses used here contain several definite pieces of single-stranded RNA. Each of these RNAs acts as a messenger. Cucumber mosaic virus (CMV) RNA 5 synthesizes a small amount of a protein, Mr 7000, in an in vitro protein-synthesizing system from wheat germ or reticulocyte. Brome mosaic virus (BMV) RNA 4 synthesizes a large amount of a coat protein under the same conditions. Both RNAs have the same 5'-cap structure and a short noncoding region (10 nucleotides in CMV RNA 5 and 9 in BMV RNA 4) between the 5' terminus and the initiation codon AUG. A sequence complementary to the 3' terminal of 18S ribosomal RNA is contained in BMV RNA 4 but is not apparent in CMV RNA 5. Formation of the initiation complex for protein synthesis by the 5'-terminal-labeled mRNA of cytoplasmic polyhedrosis virus was inhibited by the addition of unlabeled BMV RNA 4 whereas it was only slightly inhibited by unlabeled CMV RNA 5. BMV RNA 4, which has a sequence complementary to rRNA, can form the initiation complex more easily than CMV RNA5. It is concluded that an apparent complementary sequence in the 3' terminal of 18S rRNA in the 5' noncoding region of eukaryotic mRNA and the 5'-cap structure enhance the rate of initiation complex formation in protein synthesis.

Base Sequence↗
PubMed1981

5'-terminal nucleotide sequence of the messenger RNA coding for bovine corticotropin/beta-lipotropin precursor.

The complete 5'-terminal nucleotide sequence of the MRNA coding for the bovine common precursor of corticotropin and beta-lipotropin has been determined. The 5'-32P-labelled, 21-nucleotides-long, single-stranded DNA fragment complementary to a portion of the 5'-noncoding region of the mRNA was prepared from a cDNA clone and elongated by reverse transcriptase reaction with the mRNA as template. The DNA transcript formed was sequenced by the procedure of Maxam and Gilbert, and the resultant sequence was cross-checked by two-dimensional electrophoretic analysis of the partial alkaline digest of the 5'-32P-labelled mRNA. The 5'-terminal nucleotide residue was determined by two-dimensional thin-layer chromatography of the complete hydrolysis product of the 5'-32P-labelled mRNA. The nucleotide sequence determined, which partially overlaps the known sequence of the cloned cDNA, reveals the complete 5'-terminal sequence of the mRNA. This, in conjunction with our previous data, defines the complete primary structure of the mRNA. The mRNA is composed of 1098 nucleotides, including an unusually long 5'-noncoding sequence of 128 nucleotides. The presence of a 'cap' structure at the 5' terminus of the mRNA is suggested. The 5'-terminal 48 nucleotide residues of the mRNA are extremely purine-rich, having an A + G content of 83%, whereas all pyrimidine-rich segments are located downstream from there. Because the 5'-noncoding region of the mRNA contains three segments of potential secondary structure which partially overlap, it can exist in a number of alternative base-pairing configurations. However, its interaction with the 3'-terminal segment of 18-S rRNA at the site of maximal complementarity would fix the mRNA configuration in such a way as to bring the possible site of ribosome binding near the initiation codon.

Adrenocorticotropic Hormone↗