Biomedical subjects
S Harami
Publications and source records attributed to S Harami.
Prognosis of moderate dysplasia. Predictive value of selected markers in routinely prepared cervical smears.
Digital image analysis was used to extract features from 1,123 abnormal cells in 23 routinely prepared, Papanicolaou-stained cervical smears. All slides examined had a cytologic diagnosis of moderate dysplasia. Seven slides came from patients who eventually progressed to either a severe dysplasia or carcinoma in situ; the other 16 slides came from patients whose dysplasias regressed without evidence of more serious disease. Linear discriminant analysis correctly classified approximately 73% of the cells from the regression group and 66% of the cells from the progression group. Cell features contributing to the majority of variance in the model were the mean optical density of the nucleus, an autocorrelation measure, the mean optical density of the cytoplasm and the nuclear-cytoplasmic ratio. At the patient level, 13 (81.2%) of the 16 slides from the regression group and 6 (85.7%) of the 7 slides from the progression group were correctly classified. These figures yield a sensitivity of 66.7%, a specificity of 92.9%, a predictive value of a progression prognosis of 85.9% and a predictive value of a regression prognosis of 81.2%. The overall efficiency of the model was 82.6%. These preliminary results should encourage further studies for the identification of markers to indicate which patients are at high risk for progression of their cervical dysplasias.
Quantitative data on chromosome aberrations obtained by computer analysis.
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Early chromosome condensation without cell fusion. I. Preliminary results with allogeneic and xenogeneic cells.
Early chromatin condensation in interphase cells (G1) of human peripheral blood lymphocytes has been induced without virus or cell fusion by exposure to allogeneic or xenogeneic mitotic cells. The event, although similar in some ways to the phenomenon described as "premature chromosome condensation," "chromosome pulverization," and "prophasing," differs in that it does not require the presence of viruses and cell fusion before mitosis proceeds in the G1 cell. Early chromatin condensation in interphase cells induced by mitotic cells only, consists of chromatids in the early or late G1 phase of the cell cycle that are not pulverized or fragmented at mitosis. Some of the chromosomes are twice as long as the metaphase chromosomes and exhibit natural bands. Almost twice as many of these bands are produced as by trypsin treatment of metaphase chromosomes. The nuclear membrane is intact and nucleoli are present, to which some chromosomes are attached. The DNA content of the precocious chromosomes in G1 is half the amount of the metaphase complement.