Search PubMed⌕ Search

Biomedical subjects

S Haraguchi

Publications and source records attributed to S Haraguchi.

At least 91 records · Page 5Linked to original sources
PubMed1985

Excision level and indication for contralateral neck dissection in hypopharyngeal cancer surgery.

Margins of extirpated specimens of hypopharyngeal cancer were examined histopathologically. Residual cancer nest, which is most likely due to directional preponderance of the submucosal lymph flow of the hypopharyngeal cavity, was characteristically demonstrated at the superior margin and in the submucosal layer. We propose that laryngopharyngectomy be done at the extended excision level superiorly at the base of the palatine tonsil and inferiorly at the level of the 2nd thoracic vertebra and with a safety margin of at least 2 cm. The incidence of ipsilateral and contralateral neck metastasis was also histopathologically evaluated from specimens obtained by neck dissection. Contralateral neck metastasis was histopathologically confirmed in more than 30% of the cases examined and showed a difference in incidence related to histological differentiation and T classification. We conclude that bilateral neck dissection is advisable in all cases of hypopharyngeal cancer except for patients with a well-differentiated T2 lesion.

Humans↗
PubMed1985

Surgical treatment of laryngotracheal stenosis by a trough technique.

Many methods have been tried and reported in the treatment of laryngotracheal stenosis. Among them, a trough technique seems universal at present. To date, we have tried several methods of this technique, but each method had its own demerits. We used, as a cartilagenous framework, a homograft or tracheal ring cartilages for the last four cases of laryngotracheal stenosis. These tracheal ring cartilages were resected from relatively young patients with laryngeal carcinoma. Total laryngectomy was done just below the level of the fourth tracheal ring, then the fourth tracheal ring was resected and stored in 70% alcohol. The cartilage was used after it was made certain pathologically that there was no invasion of carcinoma at the third tracheal ring level. The operative procedure of this trough technique is as follows. In the first stage, a trough is created at the stenosed region and the trough is maintained by placing a Quick Set Silicone (Q.S.S.) stent mold over it. While waiting for scar formation of the tissues surrounding the trachea, the second stage reconstruction is performed four weeks after the first. The trough is closed with hinged skin flaps on both sides, and the reserved tracheal ring cartilages are sutured every 5 mm onto the outer surface of the hinged skin flaps to make a cartilagenous framework. The neck is closed with advanced neck skin flaps. A more ideal tracheal lumen is created by this technique. Our four patients are doing well two years or more after the operation.

Adult↗
PubMed1985

Scintiscanning of mucoepidermoid tumor of the parotid gland.

99mTc-O4 and/or 67Ga-citrate scintigraphies were preoperatively applied to 13 cases of parotid mucoepidermoid tumor. The resected specimens were histopathologically subdivided into three types: well (6 cases), moderately (one case), and poorly differentiated (6 cases). As the other parotid neoplasms except adenolymphoma and oxyphilic adenoma, all poorly differentiated tumors showed focal defect image in 99mTc-O4 scintiscanning (4/4). They indicated a focal hot image in 67Ga-citrate scanning at a high rate (4/6). Otherwise, well differentiated tumors were scarcely pointed out in cold image and were usually indicated as a symmetrical image (5/6) in 99mTc-O4 scanning. None of them showed a focal hot image in 67Ga-citrate scanning, but two cases indicated diffuse increased uptake and another case showed a focal defect image. The clinical prognosis of mucoepidermoid tumors is extremely correlated to the degree of cell differentiation in our prospective study. For that reason, the therapeutic method should be carefully selected in the tumors suspected of low grade malignancy. Our study suggests that RI (99mTc-O4 and 67Ga-citrate) scintigraphy is helpful in evaluating the malignancy grade of mucoepidermoid tumors.

Adolescent↗
PubMed1985

Characteristics of the epithelial component of parotid adenolymphoma.

Parotid adenolymphoma is composed of two histologic components, epithelial and lymphoid. Although some theories regarding the histogenesis of this tumor have long been disputed, there have been no definite conclusions. The purpose of this study was to clarify the origin of the epithelial components of this tumor using histochemical and immunopathological techniques, electron microscopy and a survey of HE-stained tumor sections. The results obtained indicated that the functions of the epithelial components were similar to those of the striated duct of the normal parotid gland, and morphological studies showed that the origin of the epithelial components may arise from parotid ductal inclusion in the lymphnodes in or around the parotid gland.

5'-Nucleotidase↗
PubMed1985

Strain differences in the antitumor activity of an immunopotentiator, Nocardia rubra cell-wall skeleton, in B10 congenic and recombinant mice.

An immunogenetic evaluation of the antitumor activity of the immunopotentiator Nocardia rubra cell-wall skeleton (N. rubra-CWS) has been performed using non-virus-producing tumors induced by the Schmidt-Ruppin strain of Rous sarcoma virus (RSV) in B10 congenic and recombinant mice. Live tumor cells mixed with either N. rubra-CWS or a placebo control were inoculated intradermally into the right flank of syngeneic mice. With N. rubra-CWS, the development and growth of B10 mouse tumors, S1018(B10) and B10SA2F, in B10(H-2b) mice were completely inhibited in all test mice. B10.A(5R) mouse tumor, S322(5R), was completely suppressed in 23 of 25 B10.A(5R)(H-2i5) mice, and B10.BR mouse tumor, S623(BR), was completely suppressed in 7 of 14 B10.BR(H-2k) mice. However, B10.D2 mouse tumor, S908(D2), in B10.D2(H-2d) mice and B10.A mouse tumors, S826(BA) and B10ABr1F, in B10.A(H-2a) mice were not suppressed at all by N. rubra-CWS. N. rubra-CWS showed a remarkable antitumor effect in B10 mice, not only in mixed inoculation with the tumor cells but also with intratumoral administration. These effects were not seen in B10.A mice. Peritoneal exudate cells (PEC) from B10 and B10.A strains previously treated intraperitoneally with N. rubra-CWS and/or MMC-treated tumor cells showed cytolytic and cytostatic activities on tumor cells derived from both strains. These results did not reflect the strain difference seen in the in vivo studies. Mitogenic activity of N. rubra-CWS on spleen cells, however, was significantly different among B10 congenic mice; N. rubra-CWS induced greater blastogenesis of spleen cells from B10 mice than of those from B10.A mice, corresponding to the in vivo results showing strain difference. These results suggest that the host's immunogenetic background may play an important role in cancer therapy with immunopotentiators.

Adjuvants, Immunologic↗
PubMed1983

Correlation between tumor-specific surface antigens and src gene expression in Rous sarcoma virus-induced rat tumors.

Immunization with Rous sarcoma virus (RSV)-induced mouse tumors or with SR-3Y1 or NY8-3Y1 rat fibroblasts transformed by a wild type RSV or an env gene deletion mutant of RSV induced complete transplantation resistance against an RSV-induced mouse tumor (CSA1M) in syngeneic hosts. On the other hand, 10 of 19 mice immunized with ts68-3Y1 rat fibroblasts transformed by an src gene temperature-sensitive mutant of RSV (permissive temperature: 33-35 degrees C) could not reject the CSA1M. SR-3Y1, NY8-3Y1 and RSV-induced mouse tumors expressed a common tumor-specific cell-surface antigen (TSSA) detected by a syngeneic rat antiserum against NY8-3Y1. In ts68-3Y1, expression of the TSSA was temperature-sensitive, TSSA being detected only when ts68-3Y1 was cultivated at the permissive temperature. Immunoprecipitation showed that serum from a rabbit bearing an RSV-induced tumor detected a 60-kdalton protein in cell extracts from SR-3Y1 and NY8-3Y1. Antiserum to NY8-3Y1 failed to detect this protein. These results suggest that the tumor-specific surface antigen(s) on RSV-induced mammalian tumors was coded for by an RSV src gene that was not identical with the simple form of the 60-kdalton protein identified.

Animals↗
PubMed1982

Analysis of organic acids in the hearts of patients with idiopathic cardiomyopathy by gas chromatography-mass spectrometry.

Organic acids in the hearts of patients with idiopathic cardiomyopathy, obtained by biopsy, were studied using gas chromatography--mass spectrometry. The profiling of organic acids was compared among eight cases of hypertrophic cardiomyopathy, three cases of congestive cardiomyopathy, and nine cases of other heart diseases, which were regarded as controls. It was found that almost all organic acids, especially deoxyaldonic acids of 2-deoxytetronic acid, 2,3-dideoxypentonic acid, 3-deoxy-2-C-(hydroxymethyl)tetronic acid, 3-deoxyerythropentonic acid and 3-deoxy-2-C-(hydroxymethyl)erythropentonic acid, were accumulated in large amounts in the heart in congestive cardiomyopathy, while these acids were decreased in hypertrophic cardiomyopathy. It was therefore suggested that deoxyaldonic acid metabolism in the heart in congestive cardiomyopathy is quite different from that in hypertrophic cardiomyopathy.

Carboxylic Acids↗
PubMed1982

Esophageal reconstruction with a skin-grafted pectoralis major muscle flap.

Reconstruction of the cervical esophagus is a problem in the surgical treatment of hypopharyngeal cancer. The conventional deltopectoral skin flap and the pectoralis major myocutaneous flap both have shortcomings. We developed a new technique for the immediate reconstruction of the entire cervical esophagus that utilizes the pectoralis major muscle with skin grafted directly onto it. This is especially useful in female patients who are buxom or have thick subcutaneous adipose tissue, because the anterior chest skin is not used as the skin island and, therefore, the flap is not too bulky and cosmetic problems are minimal. The breast is left unaffected with the nipple in its normal position. We report the technical details and the results in four female patients with hypopharyngeal cancer.

Esophagus↗
PubMed1982

Individually distinct tumor-specific cell surface antigen identified by monoclonal antibody on a Rous sarcoma virus-induced mouse tumor.

Hybrid cell lines were prepared by the fusion of BALB/c myeloma P3U-1 cells with the lymphocytes of BALB/c mice that were immunized with syngeneic Rous sarcoma virus (RSV)-induced tumor CSA1M cells. Three clones of the hybrid progeny (3.4B2, 3.4C6, and 3.5C11) produced cytotoxic IgM antibodies against CSA1M cells. One of the clones, 3.5C11, was chosen for analysis of the detailed specificity. Both direct cytotoxicity assays and absorption tests revealed that monoclonal antibody from 3.5C11 was positive only with CSA1M cells and that it failed to react with other tumors, including 20 RSV-induced mouse tumors, and normal cells. The 3.5C11 monoclonal antibody alone, with or without exogenous complement, was suppressive in the therapy of ip injected CSA1M tumor in syngeneic hosts, and significant prolongation in survival was seen in the treated mice. These results clearly showed presence of an individually distinct tumor-specific cell surface antigen on an RSV-induced mouse tumor.

Animals↗
PubMed1982

[Evaluation of the dopamine induced hypertension therapy for vasospasm (author's transl)].

The purpose of this study is to clarify the usefulness and the limitations of the dopamine induced hypertension therapy (DIH) in the treatment of symptomatic vasospasm secondary to aneurysmal rupture. Twelve patients suffering from ischemic complications of vasospasm were treated with DIH and large quantities of intravascular fluids. All underwent cerebral angiography and CT scan in order to ascertain if their neurological deteriorations were due to vasospasm. In 9 cases, the levels of consciousness and neurological state were improved within a few hours after DIH started. It suggests that their ischemic symptoms were dependent on their systemic blood pressure. In one case, whose vasospasm was extensive and diffuse, DIH was life-saving, however the patient remained bedridden. In 2 cases, vasospasm was too intense for DIH to prevent continued neurological deteriorations. There seems to be a correlation between the effect of DIH and the angiographic grade of vasospasm (Table 1). The ultimate results of these 12 cases were compared with those of 46 cases which suffered from symptomatic vasospasm but were not treated with DIH (Table 3). There was no significant difference between the two groups. One died of severe brain edema, which may have been aggravated by DIH. In such a case where diffuse brain edema and breakdown of blood-brain barrier are observed on CT scan, DIH can be hazardous. However, in a case where CT scan before or during the therapy shows a localized low density area of infarction, DIH can be beneficial by preventing the progression of cerebral ischemia, in spite of a possible risk of bringing about hemorrhagic infarction. Our conclusions are as follows: 1) DIH is useful in many cases involving significant vasospasm. 2) DIH is less useful in such a case whose angiogram shows extensive vasospasm throughout the internal carotid and vertebro-basilar systems, and should be performed carefully in coordination with measures to reduce intracranial pressure. DIH is expected to protect the brain tissue from ischemia by increasing blood flow through the arteries of vasospasm and collateral circulation, but should not dilate the histologically changed arteries. Further clinical studies including cerebral blood flow measurements are necessary to define precisely the effectiveness, the indication and contraindication, and the mechanism of action of DIH.

Adult↗