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S Hanazawa

Publications and source records attributed to S Hanazawa.

97 records · Page 6Linked to original sources
PubMed1982

Comparison of the adjuvant effect on antibody response of three types of carrageenan and the cellular events in the induction of the effect.

The present study was performed in order to demonstrate the adjuvant activity of antibody response of three types of carrageenan (CAR) and the cellular events involved in the adjuvant activity with CAR. CAR-induced enhancing and suppressive effect on primary antibody responses depend greatly on the dose and timing of the injection of the CAR employed. However, neither of these effects were observed in the secondary antibody responses. The maximum adjuvant effect was found when antigen and iota- and lambda-CAR (1 mg) were administered simultaneously to mice, although kappa-CAR did not cause an adjuvant effect. The effect varied with mouse strains tested and the antigen concentration used. It was demonstrated, using athymic nude mice, that the adjuvant effect is independent of T cells. Furthermore, iota- and lambda-CAR polyclonally activated B cells without the help of accessory cells such as macrophages and T cells. This study indicated that the adjuvant effect of CAR is probably independent of macrophages and T cells.

Adjuvants, Immunologic↗
PubMed1978

Kinetics and mechanisms of macrophage activation by Corynebacterium anaerobium.

Kinetics and mechanisms of macrophage activation by heat-killed Corynebacterium anaerobium (CA) in mice were investigated. The carbon clearance test revealed that the function of the reticuloendothelial system rose markedly on the 4th day after a single intravenous injection of CA and continued in a highly enhanced state until the 14th day. This activity declined gradually and dropped to a normal level around the 21st day. On the other hand, both lysosomal enzymes, beta-glucuronidase and acic phosphatase, of peritoneal macrophages decreased after the CA injection and then recovered, taking an almost inverse course to the function of the reticuloendothelial system. These results might be attributable to possible extracellular secretion of the lysosomal enzymes in accordance with macrophage activation by CA. A remarkable cytotoxicity of peritoneal macrophages, examined in vitro against L 929 cells, was detected on the 4th day following intraperitoneal administration of CA. It was maintained up to the 14th day and then declined rapidly. The mechanisms of macrophage activation by CA were also examined in vitro. CA-homogenate, heat-killed CA disrupted with an ultrasonicator, directly activated thioglycollate-induced macrophages. The macrophages were aslo activated by simultaneous treatment with both CA-homogenate and CA-sensitized spleen cells. Furthermore, the supernatant obtained from the culture of CA-sensitized spleen cells with CA-homogenate was capable of inducing activation of the macrophages. Conversely, the culture supernatant of spleen cells from CA-immunized athymic nude mice with CA-homogenate was unable to activate them. It was ascertained from the above-mentioned results that macrophages are activated initially by direct action of CA in a nonspecific way and subsequently by a soluble factor elaborated by CA-sensitized lymphocytes in an immunological way.

Acid Phosphatase↗