Biomedical subjects

S H Kinder

Publications and source records attributed to S H Kinder.

3 recordsLinked to original sources

PubMed1996

PXGEN: a General-Purpose Graphical User Interface for Protein Crystallography Experimental Control and Data Acquisition.

PXGEN is a general-purpose graphical user interface for experimental set-up and control of protein crystallography data collection. PXGEN is not linked intrinsically to any software package or proprietary hardware and should be transportable to other experimental facilities. The experimental techniques supported are single-wavelength data collection and multiwavelength anomalous dispersion. The graphical user interface runs on a UNIX-based workstation exploiting the host's power to manage multiple programs. PXGEN provides a mechanism for making data collection much easier and less error-prone. The design and implementation of PXGEN are described, which is now installed on protein crystallography beamlines 9.5 and 7.2 of the Synchrotron Radiation Source at Daresbury Laboratory.

Journal Article↗

PubMed1995

Structure determination of OppA at 2.3 A resolution using multiple-wavelength anomalous dispersion methods.

OppA is a 58.8 kDa bacterial transport protein involved in the transport of peptides across the cytoplasmic membrane of Gram-negative bacteria. It binds peptides from two to five residues in length but with little sequence specificity. OppA from Salmonella typhimurium has been cloned and expressed in E. coli and the protein cocrystallized with uranyl acetate, producing two distinct crystal forms with different uranium sites. Multiple-wavelength data collected about the uranium L(III) edge have been collected at the Daresbury Synchrotron Radiation Source (SRS) to a nominal resolution limit of 2.3 A. Maximum-likelihood phasing methods have been used in phase determination from the multiple-wavelength data giving a readily interpretable electron-density map, without any density modification. The electron-density map, calculated at 2.3 A resolution shows OppA to be a bilobal, principally beta-stranded, three-domain protein. The tri-lysine ligand molecule can be clearly seen in the peptide-binding site between the two lobes.

Journal Article↗

PubMed1994

Computing for synchrotron radiation experiments.

Computing provision is of major concern to synchrotron radiation researchers. An overview is presented of the computing facilities for synchrotron radiation work at the Synchrotron Radiation Source, Daresbury Laboratory. Data acquisition, reduction and analysis are seen as an integrated activity essential for full utilization of beam time. We discuss the evolution of data-acquisition systems from stand-alone systems of limited computing power, to a unified project computing village using state-of-the-art equipment for combined-technique, high-data-rate experiments. A brief account of software packages for data reduction and analysis is also given.

Journal Article↗