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Biomedical subjects

S Guo

Publications and source records attributed to S Guo.

At least 145 records · Page 8Linked to original sources
PubMed1998

[A micro transducer for dynamic multi-location pH monitoring in upper digestive tract].

A new transducer catheter used for dynamic multi-location pH monitoring in upper digestive tract has been developed. On the catheter several antimony electrodes and an Ag-Agcl electrode are integreted. An animal experiment compared the results of gastric aspiration and dynamic recording and demonstrated the reliability of this transducer. Clinical application has shown that this technology contributes greatly to the research on digestive physiology and to the diagnosis of functional diseases such as non-cardiac chest pain.

Animals↗
PubMed1998

[Comparison studies on pharmacodynamics of three kind of Carapax trionycis].

Three kinds of Carapax Trionycis were used in rat to prevent and treat syndrom-complex of dificiency of Yin. Compareing the improvement of symptoms of dificiency of Yin, it was found that all of them actted effectively in various degrees. The effect of ZhongHua Carapax Trionycis (ZHCT) was similar to those of Shan Rui Carapax Trionycis (SRCT), but the efficacy of Green Carapax Trionycis (GCT) was inferior to those of ZHCT and SRCT. All kinds of C. Trionycis could increase the function of responding to iriation in mice with deficiency of Yin. The intensity of ZHCT was similar to that of GCT, and superior to that of SRCT. They all could enhance phagocytic function of eticuloedothelial system obviously, promot the development of thymus in different degrees with no efect on spleen in young mice. The efficacy of increasing the immune function of ZHCT and GCT were more potent than that of SRCT. ZHCT and GCT significantly decreased the activity of SGPT in mice poisoned by CC14. ZHCT and GCT protected liver better than SRCT did.

Animals↗
PubMed1997

cis-Active Ras G2-like sequence implicated in the heterotropic activation of the deoxyadenosine kinase of Lactobacillus acidophilus R-26.

Deoxyadenosine kinase (dAK) forms a heterodimer with either deoxyguanosine kinase (dGK) or deoxycytidine kinase (dCK), and is heterotropically activated 3-5 times by dGuo or dCyd. Expressed alone, dAK is inactive and exhibits no response to dGuo or dCyd; activity and heterotropic response are fully restored upon reassociation with dGK or dCK. However, turnover of independently expressed dGK or dCK is nearly maximal, being further activated only 50-100% upon reassociation with dAK. In neither case is the heterotropic activation due to ligand-induced heterodimer formation. A proline/alanine substitution within a dAK segment homologous to loop G2 of Ras proteins yielded a heterodimer with dAK permanently cis-activated 2-fold, with a corresponding reduction in heterotropic activation by dGuo. A chimeric dAK, with 25% of its C terminus substituted by the homologous sequence from dGK, was inactive alone, and its characteristics were unchanged in the reconstituted heterodimer. Superimposing the Pro/Ala substitution on this chimera also reduced heterotropic activation by half. Cross-linking the dimer by 1,5-difluoro-2,4-dinitrobenzene was inhibited by ATP, dATP, dGTP, and dAdo, suggesting the proximity of the active site(s) to the interface. These data suggest that dAK depends on dGK or dCK in a manner resembling the reliance of Ras upon GTPase activating protein.

Amino Acid Sequence↗
PubMed1997

[Changes of plasma endothelium-derived relaxing factor and endothelin-1 in patients with chronic cor pulmonale at exercise test].

OBJECTIVE: The authers investigated the levels of plasma EDRF-nitric oxide (NO) and ET-1 in 10 cases of cor pulmonale at exercise. METHODS: Using the technologies of cardiac catheter, radioimmunization and biochemical analysis. RESULTS: The level of plasma NO was lower in group A (mean pulmonary artery pressure (mPAP) < 2.67 kPa) than in group B (mPAP > or = 2.67 kPa) at rest; the content of plasma NO increased more markedly in group A than in group B during exercise; there was no change in the content of ET-1 in both groups; mPAP strongly correlated with plasma NO or ET-1 in both groups. CONCLUSIONS: The patients with cor pulmonale at early stage may be in a state of compensated release of NO, but its release reserve has been reduced.

Aged↗
PubMed1997

[Rapid bioluminescence method for pathogen detection of nephropyelitis patient].

ATP is a kind of necessary metabolites in the living cells. We detected ATP contents in 222 bacteriuria or the urine germ by bioluminescence method. The results showed that the patient had been infected when the mean value was > 130.9 +/- 11.38 and had not when the mean value was < 32.9 +/- 2.26. The results were the same with the Gram Stain, L-form bacteriuria culture and the common bacteriuria culture. The mean values of positive specimens are higher than that of the negative specimens by the bioluminescence. Thus, it is indicated that the bioluminescence method has high sensitivity. This method can be used to detect L-form bacteriuria in the urine germ quickly and is important for early diagnosis by the doctors.

Adenosine Triphosphate↗
PubMed1997

[Variation of female migraine after menopause and its prophylaxis and treatment].

320 cases of female migraine after menopause according to the diagnostic criteria were studied by using 1:1 matched analysis. It was found that the additional symptoms increased after menopause. Eighty cases available for follow-up were divided into two groups, on which the treatment tests were done. Each patient in group I took Nilestrioli 2 mg twice a month and Perphenazine 12 mg and Doxepin 75 mg per day, while each patient in group II took Tolfenamic acid 300 mg a day. Two months after treatment, the cure rates were 57.58% in group I and 27.78% in group II. Two monthes after the cessation of therapy, 2 cases had relapses in group I. These data indicate that the decrease of estrin disorder and the additional symptoms after monopause play important roles in the change of migraine physiology, and the corresponding treatment methods are rational.

Analgesics↗
PubMed1997

[Supra-aortic arch esophageal mucosa-gastric wall anastomosis with oblique stomach invagination through esophageal bed for patients with esophageal carcinoma undergone esophagectomy].

OBJECTIVE: To reduce impairement of pulmonary function by thoracic stomach and other postoperative complications. METHODS: One hundred eighteen patients with esophageal carcinoma were treated with supra-aortic arch esophageal mucosa-gastric wall anastomosis with oblique stomach invagination through esophageal bed. RESULTS: No anastomotic leakage or gastroesophageal reflux occurred. The incidence of anastomotic stricture was 2.5%, that of operative mortality was 0.9%. Cardiopulmonary complications occurred in 11.0%. CONCLUSION: The procedure may keep the normal anatomical and physiological functions of the thoracic organs and help prevent other complications.

Adult↗
PubMed1997

[Chromosome marker--2q- during carcinogenesis and development of human lung cancer].

OBJECTIVE: To study the cytogengtic alterations during the development of human lung cancer and to clarify the genetic basis of lung cancer. METHODS: Using Fluorescence in situ Hybridization FISH, we have studied the chromosomal alterations of chromosome 2 and 3 in human bronchial epithelial cell line M, and 12 primary non-small cell lung cancer (NSCLC) specimens. RESULTS: 2q- was found in the later passage of human bronchial epithelial cell line M, and 3 out of 7 primary NSCLC specimens tested. 3p- being noted in five cases of 12 primary NSCLC patients examined was the first cytogenetic discovery and the most prominent abnormality in lung cancer. 2q- was rarely reported in human lung cancer before. But loss of heterozygosity by RFLP analysis for 2q had been reported in lung cancer. CONCLUSION: 2q- is also a common chromosome abnormality in human lung cancer. There would also be one or more putative tumor suppressor gene(s) on the long arm of chromosome 2. Loss of the gene(s) may contribute to the carcinogenesis of human non-small-cell lung cancer.

Adenocarcinoma↗
PubMed1997

[Myocardial cells action potential and contractive function in hemorrhagic shock in rabbits].

Anesthetized rabbits were subjected to a moderate degree of hemorrhagic shock. Lowering their mean arterial pressure to 5.3 KPa for 3 h, we measured the mean arterial pressure (MBP), left ventricular systolic pressure (LVSP), left ventricular maximum velocities of contraction (dp/dtmax), and myocardial electrical activity. The results of this study demonstrated that LVSP declined in parallel with MBP; that dp/dtmax dropped acutely on the instant of hemorrhagic shock; dp/dtmax went up by 48% on 15 min after shock; then dp/dtmax fell gradually, and by 3 h after shock the dp/dtmax values dropped to 20% of the baseline values; that intracellular sodium and potassium contents increased significantly in the hemorrhagic shocked rabbits; that myocardial resting potential (RP) and action potential amplitude (APH) diminished; and that action potential duration(APD), action potential repolarized 50% duration (APD 50), and repolarized 90% duration (APD 90) were markedly prolonged in the hemorrhagic shock, compared with control. The results suggested that in hemorrhagic shock myocardial cells fast sodium channel was closed while slow sodium and slow calcium channels, were opened; thus sodium and calcium infused and potassium exuded.

Action Potentials↗
PubMed1997

[Cloning and squencing of human thrombopoietin (hTPO) cDNA and it's expression in COS-7 cells].

Two hTPO cDNA segments (N-terminal and C-terminal) were amplified from human fetal liver mRNA by using separate reverse-transcription PCR reactions, and cloned into pUC19. Their sequences were identical with that previously reported. Then the full length cDNA of hTPO was obtained from the two cDNA fragments, cloned into the shuttle vector pSVK3 and transiently expressed in COS-7 cells. The activity of the expression product was demonstrated with the stimulation of CFU-Meg.

Animals↗
PubMed1997

[Follow-up study on children with obesity and hypertension].

OBJECTIVE: To study obesity development and its relationship between obesity and hypertension of children in China. METHOD: A 8-year follow-up study was conducted in 468 children aged 6 years by the criteria of hight, weight, thickness of skin fat and blood pressure. RESULTS: Obesity morbidity rose from 6 years (4.9%) to 13 years (16.2%), with the peak at 9 to 11 years. Both sexes developed obesity, which dominated in males. Children with obesity developed hypertension at age 13, the morbidity increased 3.5 times than that of those without obesity. CONCLUSION: Prevention of malnutrition is important in China but interventions to children with obesity in China are in urgent need.

Adolescent↗
PubMed1997

[Prenatal diagnosis of fetal nuchal cystic hygroma in 22 cases].

OBJECTIVE: To investigate the early diagnosis and prognosis of fetal nuchal cystic hygroma. METHODS: 72 cases of fetal nuchal cystic hygroma diagnosed in uterus were analyzed retrospectively. RESULTS: Most of the cases were diagnosed by B ultrasound between 15-26 gestational weeks. 55.9% the multipara had abnormal pregnant history, and 40% (4/10) of fetuses with karyotype performed had chrosome abnormality. Except for fetal deaths and induced abortions, 3 cases had term deliveries: one neonatal death, one was born with hygroma of 5.9 cm x 3.7 cm and transferred for surgery, and the other one with hygroma disappeared at the 37th week and baby was born at 40 week with normal appearance. CONCLUSIONS: This disease is one of common fetal morphological abnormalities. Few may recover spontaneously but most have poor outcomes.

Adult↗
PubMed1997

[The function of nitric oxide in the necrosis of avulsed skin flap in domestic pig].

To investigate the function of nitric oxide (NO) and nitric oxide synthetase (NOS) inhibitor, N-nitro-L-arginine methyl ester (L-NAME), the skin avulsion model was made in the lower extremity of pig. The methods of measurement of size of the survived flap, weighing, immunocytochemistry and hybridization in situ were employed, so that the survival surface area of flaps, tissue wet/dry weight ratio, NO content in the serum, gene expression of NO and NOS content in the flap tissue were determined, respectively. The results showed that the early gene expression of NOS was increased as well as the NO content and tissue wet/dry weight ratio (P < 0.01). After L-NAME was applied introvenously, the NO content and tissue wet/dry weight ratio were decreased (P < 0.01), and the survival surface area of flaps was enlarged (P < 0.01). It could be concluded that the NO might play a role in the development of the pathological changes as early congestion, edema and secondary necrosis in the avulsed skin flaps. The early application of L-NAME could do some good to the avulsed skin flap and protect it from further necrosis owing to the presence of NO.

Animals↗
PubMed1997

[Use of fibrin glue in the prevention of secondary anastomotic stenosis from repair and reconstruction of the injury of the bile duct].

The secondary anastomotic stenosis is often occured from the repair and reconstructive operation of the injured bile duct. It is difficult to treat and the outcome is serious. In order to prevent this complication, the fibrin glue instead of traditional suturing technique combined with inner support was used. Fifty-four hybrid dogs were divided into 3 groups. Group A received Roux-en-y choledochojejunostomy with fibrin glue; group B received Roux-en-y choledochojejunostomy, with a fibrin glue combined support left permanently in the bile duct and group C received Roux-en-y choledocholejejunostomy with fibrin glue combined a support left temporarily in the bile duct. The amount of collagen in the scar was measured at 3/4, 3, 6, 9, 12 months respectively after operation. The results showed: 1. the mature period of scar was shortened from 12 months to 9 months when fibrin glue instead of suture was used in choledochojejunostomy; 2. the mature period of scar was further shortened from 9 months to 6 months when fibrin glue combined with inner support instead of fibrin glue was used in choledochojejunostomy. The conclusions were as follows: 1. fibrin glue could facilitate the healing of wound by inhibiting the formation of scar and accelerrate the maturation of scar; 2. when the inner support was used with fibrin glue in the operation, the mature period of scar could be further shortened; 3. the mechanism of action of the fibrin glue included minimizing the injury, avoiding foreign-body reaction, modifying organization of hematoma, preventing formation of biliary fistular and enhancing intergration and cross-linkage of collagen.

Anastomosis, Roux-en-Y↗
PubMed1996

Deoxycytidine kinase and deoxyguanosine kinase of Lactobacillus acidophilus R-26 are colinear products of a single gene.

Three of the four deoxynucleoside kinases required for growth of Lactobacillus acidophilus R-26 exist as heterodimeric pairs specific for deoxyadenosine (dAK) and deoxycytidine (dCK) or dAK and deoxyguanosine (dGK). However, only two tandem genes, dak/dgk, are found, and are expressed only as dAK/dGK in transformed Escherichia coli. Sequencing peptides spanning 63% of the native dCK subunit revealed a sequence identical to that deduced from dgk (beginning MTVIVL...), except that dCK lacks residues 2 and 3 (dCK is M..IVL; dGK is .TVIVL). Also, mass spectrometry indicates that native dCK and dGK subunits are identical in mass adjusted for the first three residues. Furthermore, the native enzymes have identical isoelectric pH values, indicating an equal number of charged residues. To enable E. coli to express peptide having the native dCK sequence, codons 2 and 3 were deleted from the dgk portion of the tandem genes, resulting in expression of protein having the specificities and regulatory properties of native dAK/dCK, including heterotropic stimulation of dAK activity by deoxycytidine or dCTP (not deoxyguanosine or dGTP) and end-product inhibition of the respective activities by dATP and dCTP. Subcloning normal and mutant dgk yielded homodimeric dGK and dCK, respectively. The dCK homodimer strongly resembles human dCK, with a low K(m) for deoxycytidine, the ability to phosphorylate deoxyadenosine and deoxyguanosine at much higher K(m) values, and end-product inhibition by dCTP. Thus two distinct and specific enzymes evidently are derived from a single Lactobacillus gene. The mechanism by which this occurs in vivo has yet to be elucidated.

Amino Acid Sequence↗