Biomedical subjects

S Grobler

Publications and source records attributed to S Grobler.

23 records · Page 2Linked to original sources

PubMed1984

Lymphocyte transformation rates of homosexual men in presence of seminal plasma.

The peripheral lymphocytes of eleven healthy homosexual men and six heterosexual males were used in microlymphocyte culture systems to compare their transformation rates in the presence of PHA-M. Two homosexual men were randomly selected from the group to investigate the influence of seminal plasma fractions on their lymphocytes. The seminal plasma fractions were obtained from heterosexual normozoospermic men. Seventy two percent of the homosexual T-cell responsiveness to PHA was inhibited compared with the lymphocytes of the heterosexual men. The lymphocytes of the homosexual male seem to be less sensitive for the inhibition components present in seminal plasma.

Adult↗

PubMed1984

Sperm selection capacity of cervical mucus.

Seventeen couples who attended the Infertility Clinic for routine fertility investigations were used in the study. Complete morphological examinations were performed on the ejaculates and cervical mucus samples following coitus in order to determine the morphological selective capacity of the mucus. It was concluded that both the motility of the spermatozoa and ability of the cervical mucus to select morphological abnormal spermatozoa contributed to the high percentage of normal spermatozoa present in the cervical mucus during the post coital test.

Cervix Mucus↗

PubMed1982

Inhibition of lymphocytes by seminal plasma fractions from azo-, oligo- and normozoospermic men.

Human seminal plasma specimens from 62 patients were collected and divided into three groups according to their fertility state. Five protein peaks, obtained by column chromatography, were used in lymphocyte transformation studies to measure their effect on blast transformation rates. The results expressed as percentage inhibition showed that the immunosuppressive activity of isolated seminal plasma fractions were more marked than whole seminal plasma. There was no statistical correlation between inhibition caused by each fraction and the fertility state of the patient groups. Inhibitory components do not seem to relate to the amount of sperm cells in the ejaculate.

Humans↗

PubMedDate not supplied

The SCMC test: a screening test for spermatozoal antibodies.

The sperm cervical mucus contact test (SCMC-test) were performed on 62 couples on account of their post coital test results. Fifteen of the 17 couples (88%) who repeatedly demonstrated unexpected poor post coital tests, revealed a positive SCMC-test. In 10 cases the SCMC test results were confirmed by the tray-agglutination and mixed antiglobulin reaction. The SCMC-test is considered to be a reliable screening test for the detection of spermantibodies amongst infertile couples.

Adult↗

PubMedDate not supplied

The effect of seminal plasma on gonadotrophin secretion by sheep pituitary cells in culture.

We tested the effect of seminal plasma fractions of pooled normozoospermic and azoospermic seminal plasma on secretion of LH by sheep pituitary cells in culture. From these experiments we conclude that there is activity in seminal plasma (molecular mass 10.000-80.000) which inhibits LH secretion. This activity is not steroid-mediated and is absent in azoospermic seminal plasma. These data support our thesis that LH secretion is not only controlled by testosterone but also by spermatogenesis per se.

Animals↗