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Biomedical subjects

S Gori

Publications and source records attributed to S Gori.

42 records · Page 3Linked to original sources
PubMedDate not supplied

[Laboratory diagnosis of Pneumocystis carinii: comparison of cytological, immunofluorescent and immunocytochemical staining].

Authors review several methods for diagnosis of Pneumocystis carinii on BAL fluid in AIDS patients aiming to identify ideal routine technic. Giemsa, Gomori and Toluidine Blue staining. Direct and Indirect Immunofluorescence. Immunocytochemical methods were tested and advantages/disadvantages compared on BAL fluid from AIDS patients. Pneumocystis carinii was detected in 7 out 21 cases (33%). Gomori staining and Toluidine Blue staining were chosen for routine Pneumocystis carinii detection while IF technics are deserved as very useful in fields showing high fungal ++ contamination (inducted sputum).

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

Visceral leishmaniasis in a patient treated for polyarteritis nodosa.

We report a case of visceral leishmaniasis in a patient receiving steroids and cyclophosphamide for polyarteritis nodosa. The clinical presentation of leishmaniasis, with fever, pancitopenia and hypergammaglobulinemia may be confused with a reactivation of vasculitis or with other non-infectious conditions such as haematologic diseases. In endemic areas, the case of latent leishmaniasis must be considered and serology for Leishmania should be performed before starting immunosuppressive treatment.

Antirheumatic Agents↗
PubMedDate not supplied

[The cytological picture in pleural pneumocystosis].

We report a case of a HIV+ patient treated with prophylactic aerosolized pentamidine. The patient showed evidence of tensive right pneumothorax and slight pleural effusion. The pleural fluid was aspired from the drainage and processed in our laboratory. The cytologic examination of aspired fluid revealed abundant Pneumocystis carinii organisms. The cytologic presentation is peculiar in pleural fluid because of the presence of a large amount of trophozoites and a small number of cysts. Direct examination and Giemsa stain are suitable methods to detect trophozoites. The diagnosis can be confirmed by Gomori and Toloidine Blu stains that demonstrate the presence of isolated and scarce cysts. Pneumocystis carinii can also be recognized in Papanicolaou-stained smears as eosinophilic aggregates.

AIDS-Related Opportunistic Infections↗
PubMedDate not supplied

Cytologic and differential diagnosis of rhinosporidiosis.

Rhinosporidiosis is a mycotic infection caused by Rhinosporidium seeberi. The fungus occurs in tissues as spherules measuring 0.25-3 mm. The spherules contain endospores. Diagnosis is usually made histologically on biopsy specimens from polypoid lesions on the mucous membranes of the nasopharynx, larynx, trachea, bronchus and conjunctiva. In our experience two cases of rhinosporidiosis were diagnosed by cytology. The cytologic features are typical. On direct examination the spherules are well-circumscribed, globular structures with several endospores within. The spherules show great variability in size, up to 10-fold. The diameter ranges from 30 to 300 microns. Permanent stains for detecting R seeberi are Giemsa, Gridley and toluidine blue. Numerous mycotic infections (Coccidioides immitis, Histoplasma capsulatum, Mucor, Aspergillus, Blastoschizomya capitatus, Paracoccidioides brasilienses, Cryptococcus neoformans) can be definitively diagnosed or strongly suspected on cytology. In immunocompromised patients it is important to commence the diagnostic study on unstained material. By direct examination R seeberi organisms are identified readily by their brown color. Much more information can be gained from material stained with special stains, especially periodic acid-Schiff, in differentiating R seeberi from Coccidioides immitis.

AIDS-Related Opportunistic Infections↗
PubMedDate not supplied

Cytology in the percutaneous treatment of hydatid cysts. A report of four cases.

Four cases of percutaneous treatment of hydatid cysts are reported. Cytologic examination of the cyst fluid is important to confirm the diagnosis in patients who did not develop antibodies. Gomori stain gives the best results in identifying the three elements of a hydatid cyst: scolices, hooklets and laminated membranes. Moreover, staining for viability assessment gives information about the success of drug treatment.

Adult↗