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Biomedical subjects

S Ghosh

Publications and source records attributed to S Ghosh.

At least 685 records · Page 38Linked to original sources
PubMed1993

Keratitis, ichthyosis, deafness (KID) syndrome--the first report from India.

A patient with the keratitis, ichthyosis and deafness (KID) syndrome is described. The patient had recurrent skin infections which led to complete scalp hair loss. The nails were dystrophic. Physical development was normal; however, his intelligence was subnormal. The erythrokeratodermatous plaques over the face, trunk and extremities were characteristic and the skin in general was dry and hyperkeratotic. Palms and soles showed marked thickening with a stippled appearance.

Adolescent↗
PubMed1993

Urticaria in children.

The etiologic factors responsible for urticaria were analyzed in 44 children seen in the Urticaria Clinic, a referral center of the Dermatology Department. Of these, 23 were girls and 21 boys. Nine (20.5%) had acute urticaria and 25 (79.5%) were diagnosed as having chronic urticaria. A definitive etiology factor was identified in 18 (40%) patients. Of these, 15 had physical urticarias; in 2 foods and in 1 drug was incriminated. Probable etiologic factors were identified in 19 (43%) patients, in 5 (11.3%) of whom more than one factor was identified. In 11 (25%) children, it was not possible to establish the cause. Our study suggests that etiologic factors of urticaria are more readily identifiable in children than in adults.

Acute Disease↗
PubMed1993

Azospirillum brasilense locus coding for phosphoenolpyruvate:fructose phosphotransferase system and global regulation of carbohydrate metabolism.

Mutants of Azospirillum brasilense unable to grow on fructose include ones affected only on fructose (Fru-) and others impaired on many or all carbohydrates through interference with induction of their specific pathways (Car-). Both types of mutants could be complemented by a cosmid in broad-host-range vector pLAFR1 containing a 27.5-kb genomic insert, Car(-)-complementing activity depending on a 2.2-kb fragment, and Fru(-)-complementing activity depending on an overlapping 9.6-kb fragment.

Azospirillum brasilense↗
PubMed1993

Isolation of a glutamate synthase (GOGAT)-negative, pleiotropically N utilization-defective mutant of Azospirillum brasilense: cloning and partial characterization of GOGAT structural gene.

An Azospirillum brasilense mutant (N12) pleiotropically defective in the assimilation of nitrogenous compounds (Asm-) was isolated and found lacking in the glutamate synthase (GOGAT-). The glt (GOGAT) locus of A. brasilense was identified by isolating a broad-host-range pLAFR1 cosmid clone from a gene library of the bacterium that rectified Asm- and GOGAT- defects (full recovery of activities of the nitrogenase, the assimilatory nitrate and nitrite reductases, and the glutamate synthase). A 7.5-kb EcoRI fragment of the cosmid clone that also complemented N12 was partially sequenced to identify the open reading frame for the alpha-subunit of GOGAT. The amino acid sequences deduced from the partial nucleotide sequences of the glt locus of A. brasilense showed considerable homology with that of the alpha-subunit of GOGAT coded by the gltB gene of Escherichia coli. The genetic lesion of N12 was found within the gltB gene of A. brasilense. The gltB promoter of A. brasilense showed the presence of a consensus sigma-70-like recognition site (as in E. coli) in addition to potential NtrA-RNA polymerase, IHF, and NifA binding sites.

Amino Acid Sequence↗
PubMed1993

Subclinical hypothyroidism: a determinant of polycystic ovary syndrome.

The present study was an endeavor to explore whether and how hypothyroidism plays a role in the etiology of polycystic ovarian syndrome (PCOS). A composite picture of the hormone profile was assessed in different groups of subjects (control women and hypothyroid women with or without PCOS). Comparative analysis of the results suggests that hypothyroidism is invariably followed by a lowering of sex hormone binding globulin and an increment in the free testosterone level, but further metabolism of testosterone (T) may or may not be directed towards an overproduction of estriol (E3). The factors that dictate the route of T metabolism, and the way by which E3 acts to rescue the ovaries from the development of PCOS under the hypothyroid state are discussed.

Adult↗
PubMed1993

Internal membranes and laminates for adaptation of amperometric enzyme electrodes to direct biofluid analysis.

This study describes the use of discrete, highly selective covering inner membranes in amperometric enzyme electrodes for direct biofluid analysis. Such inner membranes when interposed between the enzyme layer and the electrode working surface has been found to be both selective, as well as biocompatible. Cellulose acetate or detergent (Triton X-100 and Tween 80) modified cellulose acetate and polyethersulphone polymeric membranes, were both found to be highly selective against electroactive solutes normally present in biofluids. The inclusion of such inner perm-selective membranes greatly enhanced the biocompatibility of the enzyme electrode and reduced the passivation of the working electrode on exposure to whole blood or serum. The study indicates that electrode drift in biological solutions may also be due to passivation of the working electrode by small diffusible surface active species.

Biocompatible Materials↗