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Biomedical subjects

S Gao

Publications and source records attributed to S Gao.

At least 181 records · Page 10Linked to original sources
PubMed1995

[Limb-salvage surgery in 60 patients with osteosarcoma].

Limb-salvage surgery plays an important role in the treatment of osteosarcoma. From 1974 to 1993, 104 patients with osteosarcoma were treated in our department. Sixty of them were treated with limb-salvage procedures. In fourty-four patients of stage II B receiving adjuvant chemotherapy, the five-year survival rate was 46.69%. The overall local recurrence rate was 15%. The infection rate was 12.9%, and mechanical complication occurred in 14.8% of the patients. The functional result was generally good. Improved concepts of en bloc resection and better reconstructive techniques suggest that limb-salvage procedures are not only possible but can provide excellent functional result in patients with osteosarcoma. Further work must be directed to reducing local recurrence and improving reconstructive techniques.

Adolescent↗
PubMed1995

[Intra- and extra-cranial arterial lesions in TIA patients].

Ninety-six patients with transient ischemia attacks were examined by transcranial Doppler (TCD), carotid color-coded ultrasonic imagine, brain computed tomographic scan (CT), compared with digital subtract angiography (DSA), MRI and MRA were done in some cases. The abnormality of CT and MRI was 30.2% and 74.1%, respectively. The most common findings were lacunar infarction or cerebral atrophy. Sixty-two cases fulfilled the TCD criteria of definitive arterial stenosis (64.5%) and three cases of arteriovenous malformation, among whom 27 patients (43.5%) carried out the examination of DSA. There was an excellent coincidence between the DSA and TCD findings, although DSA showed more vessels involved in some patients. Of the 96 cases, the intracranial arterial stenosis or occlusion was in 48.5% and the extracranial in 17.8%. The younger patients (age < 60 years) were subject to involvement of unilateral intracranial artery, but the older had predominantly the bilateral intracranial arterial and extracranial carotid or subclavian arterial lesions.

Adolescent↗
PubMed1995

[Technological studies of effects of inclusion on pericarpium Citri reticulatae volatile oil-beta-cyclodextrin].

The effects of inclusion on Pericarpium Citri Reticulatae volatile oil-beta-cyclodextrin were studied by comparing four methods saturation, grinding, liquid to liquid and liquid to gas. The utilization ratio of oil, the ratio of oil in inclusion complex and the recovery of inclusion complex were used as the criteria in evaluating the inclusion effectiveness. The results showed that the saturation method was the best.

Citrus↗
PubMed1995

[Copper needle puncturing for the treatment of cavernous hemangioma].

From the beginning of 1990 to Oct. 1993, we have treated 31 patients with cavernous hemangioma using copper needle puncturing. Three months' to one and half years' follow-up showed positive effects in all cases. The paper describes general and local reaction of the treatment, the changes in blood copper concentration and echocardiography and complications.

Adolescent↗
PubMed1995

[Effects of copper needling therapy on cavernous hemangioma assessed by echogram].

Thirty-one patients with subcutaneous cavernous hemangioma were observed by echogram before and after treatment with copper needling. Different characteristics of echogram were found in hemangioma before and after treatment. Color Doppler imaging gave great help in the diagnosis and treatment of this disease and follow-up.

Adolescent↗
PubMed1995

Pharmacokinetics of ceftizoxime in renal failure patients without dialysis.

AIM: To investigate the pharmacokinetics of ceftizoxime (Cef) in renal failure patients without any dialysis and supply the basis for a suitable clinical regimen. METHODS: Cef in plasma and urine was assayed by HPLC. RESULTS: After injecting Cef 16.7 mg kg-1, Cef concentration in blood was described as a 2-compartment open model. The main pharmacokinetic parameters were Vd 0.55 +/- 0.17 L kg-1; AUC 879 +/- 460 mg L-1 h; Cl 27 +/- 11 mL kg-1 h-1. T1/2 beta was 15 +/- 4 h. CONCLUSION: T1/2 beta in renal failure patients was about 10 times longer than that in normal volunteers. The clinical regimen should be adjusted in renal failure patients with infection, either prolonging the interval between Cef administration, or decreasing Cef dosage.

Ceftizoxime↗
PubMed1995

[Establishment of a new culture system for fetal human tracheobronchus].

Human tracheobronchial tissues were cultured in CMRL 1066 medium containing insulin, hydrocortisone, bovine pituitary extract, and trace elements, with or without fetal bovine serum, in a rocked atmosphere chamber. The morphology of the epithelium remained normal for two months after in organ culture, and then, squamous metaplasia appeared in the epithelium and maintained for more than one year. There were continuous epithelial outgrowths from the human tracheobronchial tissues during the whole duration of organ culture, and the epithelial outgrowth can be subcultured in MCDB 151 medium supplemented with growth factors and grew as a tightly packed monolayer of polygonal cells with the presence of keratin and microvilli. The advantages of organ culture and epithelial cell culture were combined in this new culture system. The new culture model has already been used for the investigation of in vitro transformation of human tracheobronchial epithelial cells by chemical carcinogens in our laboratory.

Bronchi↗
PubMed1994

DNA repair rates mapped along the human PGK1 gene at nucleotide resolution.

The repair of cyclobutane pyrimidine dimers (CPDs), DNA lesions induced by ultraviolet light, was studied at nucleotide resolution. Human fibroblasts were irradiated with ultraviolet light and allowed to repair. The DNA was enzymatically cleaved at the CPDs, and the induced breaks along the promoter and exon 1 of the PGK1 gene were mapped by ligation-mediated polymerase chain reaction. Repair rates within the nontranscribed strand varied as much as 15-fold, depending on nucleotide position. Preferential repair of the transcribed strand began just downstream of the transcription start site but was most pronounced beginning at nucleotide +140 in exon 1. The promoter contained two slowly repaired regions that coincided with two transcription factor binding sites.

Binding Sites↗
PubMed1994

The G gamma:A gamma ratios in the fetal hemoglobin of newborns of the Man ethnic group and their gamma-globin gene arrangements.

The G gamma:A gamma ratios of Hb F were determined in 331 newborn babies of the Man ethnic group in Liaoning Province, P.R. China, using polyacrylamide gel electrophoresis; in 27 cases they were also determined by reversed phase high performance liquid chromatography. The results showed that a high G gamma value (> 80%) was present in four cases (1.21%) and low G gamma values (30-48%) in six cases (1.81%); not a single case with the A gamma T [A gamma 75(E19)Ile->Thr] chain was found. Gene mapping analysis demonstrated that two babies with high G gamma values had the -G gamma-G gamma-/-G gamma-A gamma- (0.60%), and two others the -G gamma-AG gamma-A gamma-/-G gamma-A gamma- (0.60%) arrangements. Two of the four babies with low G gamma values were identified as -A gamma-A gamma-/-G gamma-A gamma- (0.60%) and the other two as -GA gamma-/-G gamma-A gamma- (0.60%). The genotype -A gamma-A gamma- has not been reported before in Chinese.

Asian People↗
PubMed1994

[A study on the boundary value of hemoglobin concentration for screening iron deficiency anemia in pregnant women].

To evaluate whether a pregnant woman is in anemia or there exist the needs to replenish iron is usually based on the measurement of hemoglobin (HB) concentration, because the evaluation of iron nutritional status has not yet been used widely in our country. Blood specimens were collected from 258 pregnant women, and concentrations of Hb, serum ferritin (SF) and free erythrocyte protoporphyrin (FEP) were determined to find out a reasonably boundary value of Hb concentration from the two different ones set by our country and WHO, respectively, and the one averaged the former two, i.e, 100g/L, 110g/L and 105g/L. False positivity and false negativity in diagnosis of iron deficiency anemia (IDA) for pregnant women were evaluated based on the measurement of SF and FEP concentrations as gold standards. Results showed that the optimal Hb concentration for preliminary diagnosis of IDA was 105g/L with maximizing Yorden index.

Adult↗
PubMed1994

[Both 987P and F41 fimbriae produced by the same enterotoxigenic Escherichia coli strain isolated from a piglet with diarrhea].

An enterotoxigenic Escherichia coli strain isolated from a piglet with diarrhea was examined for the presence of fimbriea 987P and F41 by a direct agglutination (with MAbs), an indirect immunofluorescence technique (MAbs as first antibodies), SDS-PAGE and Western blots (antisera IgG as probes). Results of these techniques revealed that both 987P and F41 fimbrial adhesins were produced by the same strain, not by separate ones.

Adhesins, Escherichia coli↗
PubMed1994

[Determination of norfloxacin, an active dissociation product of silver norfloxacin, in guinea pig plasma and tissue by high performance liquid chromatography with column-switching].

A column switching HPLC method was established for the determination of norfloxacin, and active dissociation product of silver norfloxacin, in guinea pig plasma and tissues. The pre-treating column (50 mm x 5 mm ID) and analytical column (150 mm x 5 mm ID) were packed with mu-Bondapak C18, 37-50 microns and YWG-C18, 10 microns, respectively. A mixture of CH3OH-0.008 mol/L phosphate buffer-0.05 mol/L tetrabutylammoniun bromide (25:75:4) was used as the analytical mobile phase with a flow rate of 1 ml/min. The pre-treating mobile phase was 0.008 mol/L phosphate buffer with a flow rate of 3 ml/min. UV detection was made at 280 nm. The results were linear within a range of 2-128 micrograms/ml norfloxacin for plasma. The lowest detection limit was 0.25 microgram/ml for plasma. The mean recovery of the method was 102% with a relative standard deviation of less than 7%.

Administration, Topical↗