L2C leukemia: a model of human acute leukemia.
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Age Factors↗
Biomedical subjects
S G Murphy
Publications and source records attributed to S G Murphy.
The chronic lymphatic leukemia lymphocyte: correlation of functional, metabolic, and surface immunoglobulin characteristics.
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Antibody Specificity↗
Human lymphocyte membrane immunoglobulin as a reflection of maturation.
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Adolescent↗
Transfer factor: isolation of a biologically active component.
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Cell Separation↗
Characterization and pathophysiology of serum glycoprotein alterations in malaria.
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Acetates↗
Endotoxin-free biologically active component of Escherichia coli.
The proteinaceous component of gram-negative bacteria, which has been termed "protodyne," enhances nonspecific host resistance while eliciting a slight pyrogenic response equivalent to 0.2% that of a typical endotoxin. Since this material still contains small amounts of carbohydrate and lipid, it was imperative to establish that its biological activities are not the result of endotoxin contamination. Evidence that the protective activity of protodyne does not result from endotoxin contamination has now been obtained by an evaluation of the Pronase digestion products of this substance. These digestion products were found to be nonpyrogenic and to contain no measurable amount of 2-keto-3-deoxyoctonate, an essential component of bacterial lipopolysaccharides.
Animals↗
Tetanus toxin and antigenic derivatives. I. Purification of the biologically active monomer.
A procedure for the isolation of pure tetanus toxin in a lethal monomeric form was developed based on the extraction of whole cells and chromatographic techniques. A crude extract of toxin was obtained by hypertonic extraction of cells from a 72-hr culture of Clostridium tetani Massachusetts strain. The extract was precipitated with ammonium sulfate and further purified by sequential use of ion-exchange chromatography and gel filtration. The degree of purification obtained by the fractionation procedures was monitored by polyacrylamide gel electrophoresis. The pure toxin has an average specific activity of 150 x 10(6) mouse MLD per mg of N and 3,000 Lf per mg of N. Immunological purity was demonstrated by a single line on both immunoelectrophoresis and agar double diffusion. One band was obtained on polyacrylamide electrophoresis, as was a single symmetrical peak in the ultracentrifuge and on Sephadex G-100 chromatography. The pure protein has an absorbancy ratio (280/260 mmu) of 2.1 in phosphate buffer (pH 7.5).
Chromatography, Gel↗
Tetanus toxin and antigenic derivatives. II. Effect of protein and formaldehyde concentration on toxoid formation.
Tetanus toxoid formation was examined under varying conditions. Products of the reaction depended upon the concentration of both formaldehyde and toxin. High concentrations of protein and formaldehyde favored the formation of large polymers, whereas low concentrations yielded smaller polymers and monomer. The monomer had an observed S value of 7.1, whereas the polymers ranged from 10.1S to 110S. The cross-linking between toxin molecules to form toxoid polymers appeared to be random.
Chromatography, Gel↗
Nitrogen metabolism of some strains of Rhizobium japonicum having different nodulating capacities.
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Culture Media↗