Biomedical subjects

S G House

Publications and source records attributed to S G House.

9 recordsLinked to original sources

PubMed1986

High fat diets varying in ratios of polyunsaturated to saturated fatty acid and linoleic to linolenic acid: a comparison of rat neural and red cell membrane phospholipids.

The polyunsaturated-saturated (P/S) fatty acid, and linoleic-linolenic (18:2n6/18:3n3) acid ratios of diets fed to rats were varied independently during pregnancy, lactation and, in the young, for 8 d after premature weaning. The intent was to alter the proportion of membrane phospholipid fatty acids derived from 18:2n6 and 18:3n3 in the developing rat, and to compare changes in very-long-chain polyunsaturated fatty acids in membranes from the central nervous system with those of the red blood cell. All experimental diets contained 40% of energy from fat. Similar relative changes in phosphatidylethanolamine (PE) and phosphatidylcholine (PC) fatty acid pattern occurred in both neural and red blood cell membranes when dietary 18:2n6/18:3n3 was increased from 7 to 240. Docosapentaenoate (22:5n6) from 18:2n6 increased, and docosapentaenoate (22:5n3) and docosahexaenoate (22:6n3) from 18:3n3 decreased in both types of membranes. On the other hand, P/S ratios of 0.3 and 1.6 at a constant ratio of 18:2n6/18:3n3 produced identical membrane phospholipid fatty acid patterns. Both red blood cell and neural membranes show the same relative effects of modification of dietary lipids on the composition of very-long-chain polyunsaturated fatty acids.

Animals↗

PubMed1986

Oral and intraperitoneal administration of N-acetylneuraminic acid: effect on rat cerebral and cerebellar N-acetylneuraminic acid.

Rat pups were administered N-acetylneuraminic acid (NANA) by i.p. injection or via a feeding catheter for eight consecutive days beginning on d 14 of life. All pups were given the identical dose: 1.0 mg on d 1 and 2 and 1.2 mg on the remaining days, or approximately 20 mg/kg body weight per day. A control group was injected i.p. with glucose instead of NANA. On the morning of d 25, pups were decapitated, and the heads were frozen immediately in liquid nitrogen. The brains were later dissected and analyzed for both cerebral and cerebellar ganglioside and glycoprotein NANA. Administration of NANA by both oral and i.p. routes resulted in significantly more cerebral and cerebellar ganglioside and glycoprotein NANA than did glucose injection (with the exception of cerebellar glycoprotein NANA after NANA intubation). There were no significant differences in NANA concentration in these brain fractions for the two routes of NANA administration.

Administration, Oral↗

PubMed1983