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Biomedical subjects

S Futaki

Publications and source records attributed to S Futaki.

139 records · Page 8Linked to original sources
PubMed1977

Immunological properties in staphylococcal toxic epidermal necrolysis.

The analysis of host defenses in 4 patients with staphylococcal toxic epidermal necrolysis revealed normal serum immunoglobulin levels, but a deficiency in cell-mediated immunity as detected by the tuberculin reactions. We suspected that the transient deficiency of cell-mediated immunity may have contributed to the development of this disease.

Child↗
PubMed1977

A bacteriological study on children with staphylococcal toxic epidermal necrolysis in Japan.

From 1973 to 1976, 21 patients with staphylococcal toxic epidermal necrolysis were admitted to the Jikei University Hospital in Tokyo. Of all of them were isolated coagulase-positive Staphylococcus aureus from various sites. In Japan, unlike in Europe and America, phage group II cocci were found in few cases. On the other hand, S. aureus belonging to other phage groups than group II (I, III mixed group and III group) were very commonly isolated. All the isolates produced exfoliatin which was capable of producing exfoliation in newborn mice. The type (A or B) of exfoliation which was detected by the antiserum agar method did not correlate to the severity of this disease.

Child↗
PubMed1975

Two serotypes of exfoliatin and their distribution in staphylococcal strains isolated from patients with scalded skin syndrome.

Two serotypes of exfoliatin, A and B, previously reported by Kondo et al. (1974) were examined for their presence in 43 strains of Staphylococcus aureus, most of which were isolated from patients with Ritter's disease and impetigo. The tested strains consisted of 24 strains of phage group II and 19 strains not of phage group II. Twenty-four strains were found to produce an exfoliatin of either type A or type B, but 16 strains produced both types and 3 strains produced neither. No relationship was found between the serotype of an exfoliatin and the phage type of the exfoliative strain, although the single producers of exfoliatin A were all found to belong to phage group II and those of exfoliatin B to the other phage group.

Bacteriophage Typing↗
PubMedDate not supplied

Stearylated arginine-rich peptides: a new class of transfection systems.

Membrane-permeable arginine-rich peptides, such as HIV-1 Tat-(48-60), HIV-1 Rev-(34-50), and flock house virus (FHV) coat-(35-49), have been shown to possess the ability to transfect COS-7 cells with luciferase-coding plasmid as efficiently as polyarginine (MW 5000-15 000) and polylysine (MW 9800). Not only these virus-derived cationic peptides but also oligoarginines of 4-16 residues were found to be able to transfect cells. In the case of the Tat, FHV, and octaarginine peptides, N-terminal stearylation of the peptides increases the transfection efficiency by approximately 100 times to reach the same order of magnitude as that of LipofectAMINE, one of the most efficient commercially available transfection agents. Also, a certain correlation was observed between the transfection efficiency of stearyl-(Arg)n peptides (stearyl-Rn: n = 4, 8, 12, 16) and the membrane permeability of the corresponding (Arg)n peptides (Rn).

Amino Acid Sequence↗
PubMedDate not supplied

Specific cytotoxicity of antibody to YAL-198, a sperm antigen peptide, to murine zygote.

Active immunization with the peptide segments rSMP-230 and YAL-198, corresponding to the hydrophilic extracellular domain of two human sperm antigens (rSMP-B and YWK-II, respectively), reduced fertility in female rats by different mechanisms. The anti-rSMP-230 antibody interferes with human and murine fertilization, and the anti-YAL-198 antibody blocks the development of mouse embryos. The authors examined in vitro at which stage the antibodies to rSMP-230 and YAL-198 were cytotoxic to murine embryos up to morula/blastocyst stage. Anti-rSMP-230 antibody was not cytotoxic to any stages. On the other hand, the anti-YAL-198 antibody arrested the growth of embryos at the 2-cell stage but not at more advanced developmental stages. When the anti-YAL-198 antibody was used, spotty staining was observed only on the surfaces of embryos that had arrested at the 2-cell stage. Unstained embryos, however, continued to develop normally. In contrast, the anti-rSMP-230 antibody stained murine sperm but failed to stain murine ova and embryos. The present results suggest that the human sperm components rSMP-B and YWK-II play important roles in sperm-egg interaction and early development of the embryo, respectively.

Animals↗
PubMedDate not supplied

Immunoglobulin binding factor in human seminal plasma: immunological function.

Human seminal plasma contains a protein with an estimated molecular weight of 16 kd that binds serum immunoglobulin gamma (IgG) and is named IgG binding factor (IgBF). Purified IgBF specifically suppressed pokeweed mitogen-induced lymphocyte blastogenesis, having little or no effect on lymphocyte blastogenesis stimulated with phytohemagglutinin or Concanavalin A; antibody-dependent cell-mediated cytotoxicity; natural killer cell activity; or complement-dependent cytotoxicity of antibodies against sperm. It would appear that IgBF may suppress activation of B cells in the male and female genital tract.

Antibody-Dependent Cell Cytotoxicity↗