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Biomedical subjects

S Fuchs

Publications and source records attributed to S Fuchs.

At least 343 records · Page 19Linked to original sources
PubMed1974

Thymus independence of a collagen-like synthetic polypeptide and of collagen, and the need for thymus and bone marrow-cell cooperation in the immune response to gelatin.

Several inbred mouse strains were screened for their ability to respond to the ordered periodic collagen-like polymer (Pro-Gly-Pro)(n), to the random copolymer (Pro(66), Gly(34))(n), to the protein conjugate Pro-Gly-Pro-ovalbumin, to rat tail tendon collagen, rat tail tendon gelatin, and to Ascaris cuticle collagen. Differences were obtained in the magnitude of the antibody titers towards the above immunogens among the strains tested. The level of the response to the ordered polymer (Pro-Gly-Pro)(n) was not similar to that towards the random (Pro(66), Gly(34))(n), confirming differences in the antigenic determinants of the two immunogens. The role of the thymus in the immune response to (Pro-Gly-Pro)(n) and (Pro(66), Gly(34))(n) as well as to two collagens and gelatin, was studied in order to find out a possible correlation with the structural features of the immunogens. Heavily irradiated recipients were injected with syngeneic thymocytes, marrow cells, or a mixture of both cell populations and were immunized with the above-mentioned antigens. An efficient immune response to the ordered collagen-like (Pro-Gly-Pro)(n) was obtained in the absence of transferred thymocytes. The thymus independence of (Pro-Gly-Pro)(n) was confirmed when thymectomized irradiated mice were used as recipients. In contrast with these results, cooperation between thymus and marrow cells was necessary in order to elicit an immune response to (Pro(56), Gly(34))(n). Similarly, the immune response to the triple helical collagen was found to be independent of the thymus, whereas for an effective response to its denatured product, gelatin, thymus cells were required. These findings indicate that a unique three-dimensional structure of immunogens possessing repeating antigenic determinants plays an important role in determining the need for cell to cell interaction in order to elicit an antibody response.

Animals↗
PubMed1974

Antibodies to yeast phenylalanine transfer ribonucleic acid are specific for the odd nucleoside ) in the anticodon loop.

Antibodies with specificity to a single species of tRNA were elicited in a goat by immunization with a glutaraldehyde conjugate of yeast phenylalanine transfer RNA with bovine gamma globulin. The specificity of the antibodies was studied by a radioimmunoassay measuring the direct binding of [(3)H]tRNA(Phe) or the inhibition of the binding. The antibodies formed are predominantly directed towards the characteristic highly modified nucleoside Y, which is located right next to the anticodon. The antibodies bind specifically to tRNA(Phe), to oligonucleotides derived by enzymatic digestion from the anticodon loop of tRNA(Phe), and to the Y nucleoside itself. tRNA species which do not contain Y in their sequences, or tRNA(Phe) from which the Y base has been excised, do not bind to the antibodies. Yeast tRNA(Phe) can be separated from other tRNA species with an immunoadsorbent of antibodies to tRNA(Phe).

Animals↗