Search PubMed⌕ Search

Biomedical subjects

S Fink

Publications and source records attributed to S Fink.

At least 127 records · Page 7Linked to original sources
PubMed1983

Effect of phytohemagglutinin-stimulated human lymphocytes on isolated rat atria. Participation of lipoxygenase products of arachidonic acid metabolism.

The effect of phytohemagglutinin (PHA) stimulated human lymphocytes on the contractile activity of isolated rat atria was studied. Increased isometric developed tension (IDT) and higher frequency of contractions (FC) were observed shortly after contact of PHA-activated lymphocytes with spontaneously beating rat atria. Since pre-exposure of the lymphocytes to PHA was not necessary and the pharmacologic effects were demonstrated immediately after addition of the lectin, division of the effector cells as a requisite for their action was excluded. Experiments performed with the antibiotic crystallized from Streptomyces chartreusenis, Ca2+-ionophore A-23187, yielded similar effects. Lymphocyte fractionation studies showed that T-lymphocyte-rich and T-lymphocyte-depleted cell fractions had opposite effects on IDT and FC. The inotropic and chronotropic effects were not modified by (-)-propranolol or antihistaminics. Inhibitor of the synthesis of prostaglandins enhanced the action of PHA-activated lymphocytes but inhibitors of the lipoxygenase pathway: 5,8,11,14-eicosatetraynoic acid (ETYA) and nordihydroguaiaretic acid (NDGA) prevented the increment of IDT and FC. Also, FPL55712, an antagonist of SRS-A abolished the positive inotropic and chronotropic effects of PHA-activated lymphocytes on rat atria. Therefore, a central role for SRS-A in this reaction is suggested.

Animals↗
PubMed1982

Technique for implantation of the Toronto-Western catheter at the Booth Memorial Medical Center.

The proper placement of the chronic peritoneal dialysis catheter is pivital to the early, as well as continued success of dialysis therapy with Continued Ambulatory Peritoneal Dialysis. We describe our current technique for the implantation of the Toronto-Western catheter. The success of any particular technique is dependent upon the incidence of complications. The latter is described elsewhere in this symposium.

Anesthesia, Local↗
PubMed1977

Further studies on the identification of the subcomponents of the first component of complement after affinity chromatography of human serum on IgG-sepharose.

Affinity chromatography of serum on IgG covalently linked to Sepharose results in the retention of the proteins of the first component of complement (C1). A fraction called pool II is eluted from this column with 0.025 M EDTA and has previously been shown to contain C1s and a novel protein believed to be part of the C1 complex and called C1t. C1r has now been located in pool II and these three proteins were purified by DEAE cellulose chromatography. C1r and C1s were recovered in the proenzyme form and their identity was established by SDS polyacrylamide gel electrophoresis before and after reduction and alkylation, and on the basis of their esterolytic activities toward different substrates. The properties of C1r from pool II are contrasted with those of the protein recovered from the pool III eluate of the affinity column and previously thought to be C1r.

Chromatography, Affinity↗