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Biomedical subjects

S Filosa

Publications and source records attributed to S Filosa.

44 records · Page 3Linked to original sources

The differentiation and proliferation of follicle cells during oocyte growth in Lacerta sicula.

The follicular epithelium of the lizard oocytes undergoes structural and morphological modifications throughout oocyte growth. During this process the number of follicle cells increases and the epithelium acquires a multilayered and polymorphic organization which is characterized by the appearance of large follicle cells (intermediate and pyriform cells). The number of large cells also increases during oocyte growth and this increase parallels that of small cells. However, only the small cells become labelled one hour after [3H-]thymidine administration. Large cells have been found labelled after a longer period of time, i.e. 4--5 months after isotope injection. All these results together indicate that large follicle cells arise from the differentiation of small cells.

Animals↗

Intercellular bridges between follicle cells and oocyte during the differentiation of follicular epithelium in Lacerta sicula Raf.

Intercellular bridges first appear during lizard oogenesis when follicles are rather small (150 microgram in diameter); at this stage they form connecting links between the oocyte and follicle cells, which have not yet differentiated into pyriform cells. Later on, when the follicles have become larger (1 mm) and the follicular epithelium appears constituted by 3 types of cells (small, intermediate and pyriform cells) they form connecting links between the oocyte and both intermediate and pyriform cells. The establishment of intercellular bridges between pyriform cells and the oocyte precedes the complete differentiation of the former, which excludes the possibility that the fusion between pyriform cells and oocyte occurs only after these cells are completely differentiated. In still larger follicles (up to 2 mm in diameter), during the degeneration of the pyriform cells, the occurrence, inside the bridges, of mitochondria and other cytoplasmic material suggests that these cells at the end of their function transfer their contents into the oocyte.

Animals↗

Intercellular bridges in lizard oogenesis.

Study of the germinal epithelium in the adult lizard shows that the germ cells constitute clusters of synchronized cells interconnected by intercellular bridges. Such bridges interconnect oogonia as well as early meiotic prophase oocytes (zygo-pachitene). Besides true intercellular bridges in oocytes, there are plasma membrane interruptions forming large zones that ensure cytoplasmic continuity between adjacent cells. In early diplotene, germ cells are isolated. Later, during auxocytosis, when the polymorphic follicular epithelium around the oocyte starts differentiating, intercellular bridges appear between the follicle cells and oocyte. No relationship is observed between the intercellular bridges found in the germinal epithelium and those found between the follicle cells and oocyte.

Animals↗

DNA haplotypes in the G6PD gene cluster studied in the Chinese Li population and their relationship to G6PDCanton.

In an effort to investigate the subtelomeric region of the X chromosome among Orientals, five DNA sites in the F8C and G6PD genes were analyzed in a sample of 46 chromosomes belonging to the Chinese Li population, an ethnic group characterized by a high prevalence of G6PD deficiency. The DNA sites analyzed, which are highly polymorphic in other populations, have a low degree of heterozygosity in the Li sample and, furthermore, the distribution of the corresponding haplotypes is very different from that previously observed in Italian populations. Interestingly, three unrelated Li G6PD-deficient variants analyzed at the DNA level have the 1376G-->T mutation characteristic of G6PDCanton and they share the same haplotype, including the sites mentioned above, as well as eight DNA polymorphisms in the red/green color vision pigment genes located proximal to G6PD on chromosome X.

Adult↗