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Biomedical subjects

S Ferrone

Publications and source records attributed to S Ferrone.

At least 451 records · Page 25Linked to original sources
PubMed1977

Cytotoxic antibodies to cultured melanoma cells in the sera of melanoma patients.

By means of the complement-dependent microcytotoxicity test, cytotoxic antibodies to melanoma cells in long-term culture were detected in 34 of 90 sera from melanoma patients. The incidence of cytotoxic antibodies in melanoma patients was significantly greater than in subjects free of malignant disease but not significantly greater than in patients with other types of cancer. The sera were cytolytic to melanoma cells only in conjunction with rabbit complement, and they reacted with the pabel of melanoma cells in a distinct fashion. No association was found between presence of cytotoxic antibodies and the occurrence of metastasis.

Antibodies, Neoplasm↗
PubMed1977

Immunogenicity of HLA antigens purified from serum.

The immunogenic properties of HLA-A9 antigens isolated from serum have been evaluated. A9 antigens at various stages of purification can elicit the formation of cytotoxic antibodies which become operationally specific to A9 either after absorption of the xenoantisera with cultured human lymphoid cells or human red blood cells, or after dilution of xenoantisera with human serum. A9 xenoantisera do no affect mixed lymphocyte reactions between allogeneic lymphocytes carrying A9, suggesting that coating of antigens of the A locus does not impair the functional activity of lymphocytes in the mixed lymphocyte reaction.

Animals↗
PubMed1977

Humoral immunity in kidney transplant recipients.

Sera obtained from kidney transplant recipients at various times after the graft were investigated for the presence of cytotoxic antibodies to B and T human lymphoid cells and for their ability to inhibit mixed lymphocyte reaction between allogeneic lymphocytes. More than 50% of sera from 35 such patients contained cytotoxic antibodies to B lymphocytes and 27 of 33 sera blocked and miced lymphocyte reaction by 25 to 75%. A statistically significant association between the presence of cytotoxic antibodies to B cells and the ability to block the mixed lymphocyte reaction was found in the group of patients with functioning grafts. However, the presence of cytotoxic antibodies to B lymphocytes did not correlate with the outcome of the graft.

B-Lymphocytes↗
PubMed1977

Cytotoxic antibodies to cultured human lymphoid cells in polytransfused thrombocytopenic patients: association with reduced survival of allogeneic transfused platelets.

Four out of 8 thrombocytopenic patients without detectable cytotoxic antibodies to human peripheral lymphocytes contained cytotoxic antibodies to cultured human lymphoid cells. The presence of such antibodies was associated with reduced survival of infused allogeneic platelets in these patients. The antibodies reacted in a distinct fashion with a panel of cultured human lymphoid cells and are directed to B cell antigens. Cytotoxic antibodies to cultured human lymphoid cells did not react in vitro with platelets suggesting that the antibodies play no significant role in the accelerated destruction of infused allogeneic platelets, although their presence predicts it. Therefore, screening of sera with cultured human lymphoid cells appears to be a useful test in addition to those now used to select patients for transfusion of allogeneic platelets. Sera from polytransfused patients without cytotoxic antibodies to peripheral lymphocytes may be a useful source of antibodies to B cell antigens.

Antibodies↗
PubMed1977

Immunogenicity of human B cell antigens solubilized from cultured human lymphoid cells.

Antigens solubilized from culured human lymphoid cells WI-L2 and RPMI 1788 were partially purified by ultracentrifugation on a KBr gradient. These antigens injected into rabbits produced xenoantibodies which after absorption with melanoma cells became specific to B cell antigens. Three such xenoantisera were submitted to the Second Histocompatibility Workshop of the Americas and reacted much like alloantisera to B cell antigens against a large panel of B peripheral lymphocytes and cells from patients with chronic lymphocytic leukemia. Xenoantisera to B cell antigens inhibited the mixed lymphocyte reaction, but did not affect the mitogenic activity of phytohemagglutinin or the functional properties of C3 receptors, monkey red blood cell receptors, or T cell receptors.

Antibodies↗
PubMed1977

Biologic and chemical characterization of HLA antigens in human serum.

HLA antigens of both the A and B loci were shown to be associated with the high density lipoprotein fraction of serum prepared by ultracentrifugal flotation. HLA-A9 antigens were purified 100-fold with essentially complete recovery by a simple procedure of high density lipoprotein preparation involving precipitation with polyanions and ultracentrifugal flotation. The purified lipid-associated antigen was immunogenic since it elicited the formation of cytotoxic xenoantibodies in rabbits. Serum HLA-A9 antigens were found by immunoprecipitation and gel electrophoresis to consist of a 45,000 m.w. heavy chain associated with beta2-microglobulin. The size of the HLA-lipid complex (less than 190,000 m.w.) and of the HLA-deoxycholate complex (less than 102,000 m.w.) suggests that HLA antigens are shed into plasma as a complex of a single HLA molecule and a single beta2-microglobulin chain, associated with boundary lipid.

Antibodies↗
PubMed1977

Cell surface markers on epithelial-Burkitt hybrid cells superinfected with Epstein-Barr virus.

Attempts were made to superinfect two epithelial-Burkitt hybrid cell lines, designated D98/HR-1 and D98/Raji, with Epstein-Barr virus (EBV) and to investigate the expression of some cell surfacr markers including histocompatibility antigens, and the presence of B-cell markers, such as receptors for the third complement component and for monkey red blood cells. Successful superinfection of D98/HR-1 cells with EBV was made evident by the expression of early antigen and, to a lesser extent, virus capsid antigen. Only a rare D98/Raji cell was found to be positive for early antigen. The histocompatibility antigens of the parental cell lines D98, HR-1, and Raji were expressed on the surfaces of the hybrid cells. Receptors for third complement components b and d were not detected on the hybrid cells or on the D98P OR HR-1 cell lines; they were found, however, on the Raji cells, indicating that EBV receptors and complement receptors can be separated. The significance of the infection of the hybrid cells with EBV and the expression of cell surface markers is described.

Antigens, Viral↗
PubMed1977

Approaches for the isolation of biologically functional tumor-associated antigens.

Melanoma-associated antigens were isolated from human melanoma cells in long-term tissue culture and from the spent culture fluid of these cells propagated in chemically defined, serum-free media. The 3 M KCl extracts from such cells and their concentrated spent culture media elicited specific delayed cutaneous hypersensitivity reactions in patients with malignant melanoma but not in patients with other neoplasms. HLA antigens present in these extracts could be specifically removed by ultracentrifugation in KBr at a density of 1.23 g/ml. Purification of melanoma-associated antigens was achieved by this step, followed by ion-exchange chromatography and preparative isoelectric focusing on Pevikon C870. Another approach is described for the isolation of carcionembryonic antigens from metastatic lesions with an approximately 70% yield utilizing the least denaturing procedures, which avoid lyophilization and involve essentially 0.9% NaCl solution extraction, specific adsorption, elution from concanavalin A Sepharose, and subsequent gel-exclusion chromatography on Ultrogel AcA 22. For effective isolation of carcinoembryonic antigens freely shed from cultured cells derived from a primary colon tumor, a system was devised based on the use of Amicon hollow fiber culture units, in which cultured tumor cells were introduced in the extracapiliary spaces of such a unit. The extracapillary fluid, containing carcinoembryonic antigens but no fetal calf serum components, is removed and further purified by affinity chromatography.

Animals↗
PubMed1977

The immunologic and molecular profiles of HLA antigens isolated from urine.

Human urine was shown to be a good source for the isolation of immunologically functional HLA-A9 antigens. The use of complex solubilization procedures can be avoided since the antigens are present in soluble form and are not complexes with membrane fragements. Purification in excess of 400-fold could be achieved by the application of cellulose ion exchange chromatography, isoelectric focusing, and acrylamide gel electrophoresis. The purified HLA-A9 antigen is composed of a glycoprotein of m.w. 38,000 and beta2-microglobulin, a peptide of m.w. 12,000. HLA-A9 antigens isolated from urine proved to be immunologically functional since they not only reacted specifically with anti-HLA-A9 alloantibody but also elicited anti-HLA-A9 xenoantibodies. These antibodies when covalently attached to Sepharose 4B specifically bound HLA-A9 antigens isolated from both serum and urine.

Arginine↗