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Biomedical subjects

S Fekete

Publications and source records attributed to S Fekete.

At least 73 records · Page 4Linked to original sources
PubMed1992

Bioavailability of methionine-hydroxy-analog free acid and S-methyl-methionine in the growing rat.

The biological utilization of DL-methionine (MET), DL-methionine-hydroxy-analog and DL-S-methyl-methionine-sulphonium-chloride (SMM) was tested in rat growth assay. Weight gain, feed efficiency, protein efficiency ratio (PER), net protein ratio (NPR) and net protein utilization (NPU) were applied as criteria. A test diet containing soybean meal as sole protein source was fortified with MET, MHA or SMM at 1.5 and 3.0 g/kg levels, respectively. All fortifications had a significant (p < 0.05) positive influence on weight gain, feed conversion and protein utilization. SMM showed the highest activity. MET and MHA were almost equally well utilized at 3.0 g/kg fortification level, however MHA proved slightly less efficient at 1.5 g/kg level than MET.

Animal Feed↗
PubMed1992

Feeding animal fats to sheep.

In an experiment lasting 7 weeks, 18% of the calculated energy requirement of 5 nonpregnant ewes was met by giving natural fats of animal origin mixed in the ration (at a level of 6.6%). Changes in certain blood and blood plasma parameters of lipid and energy metabolism were monitored at 5 time-points during the experiment. Samples of rumen fluid were taken three times for determining the concentrations of volatile fatty acids (VFA). Rumen fermentation was studied by in sacco method. Total lipid (TL) content of the liver and fatty acid composition of the liver tissue and subcutaneous adipose tissue (from the tailhead) were analyzed at the beginning and at the end of the experiment. At the concentration used in this experiment, fat supplementation caused neither digestive disturbances nor any other adverse changes in the animals' health status. It did, however, exert a significant influence on blood plasma lipid composition. TL and total cholesterol (TCh) concentration increased and blood glucose level decreased. A rise in triglyceride (TG) content was accompanied by a drop in free fatty acid (FFA) concentration. The in sacco experiments and volatile fatty acid (VFA) levels in the rumen fluid suggested an impaired crude fibre digestion in the rumen. At the same time, fat supplementation enhanced rumen proteolysis. The TL content of liver samples did not exceed the physiological limit. The liver biopsy samples had decreased myristic acid and increased stearic and oleic acid concentrations. No change occurred in the fatty acid composition of the fat depots.

Animal Feed↗
PubMed1991

Serum alpha 2-HS glycoprotein concentration in patients with hematological malignancies. A follow-up study.

We observed significantly reduced serum alpha 2-HS glycoprotein concentrations in patients with acute lymphocytic, acute nonlymphocytic, chronic granulocytic and chronic myelomonocytic leukemias, Hodgkin's and non-Hodgkin's lymphomas, myelofibrosis, and multiple myeloma, but not in patients with chronic lymphocytic leukemia and polycythemia vera, as compared with healthy controls. We followed the serum level of the protein for 18 months. Patients with infectious complications, those receiving cytostatic treatment, and those in the preterminal period had further reduced serum alpha 2-HS glycoprotein levels. The reduction of serum alpha 2-HS glycoprotein concentration was primarily due to decreased production caused by infiltration of the liver, a hepatotoxic effect of cytostatic treatment, and, to a lesser degree, to increased consumption. We found statistically significant negative correlations between serum alpha 2-HS glycoprotein concentration and erythrocyte sedimentation rate, serum aspartate aminotransferase and alkaline phosphatase activities, and IgG and IgM concentrations. The determination of the alpha 2-HS glycoprotein concentration is useful for the assessment and follow-up of the clinical status and therapy of patients with hematological malignancies and also has prognostic significance.

Adolescent↗
PubMed1990

Evaluation of the energy value of rabbit feeds: a critical review.

An analysis is given of the methods actually used for the determination and calculation of the energy value of rabbit feeds and feedstuffs. The apparent digestible energy (DE) system is recommended. Standardization of the applied methodology for in vivo determination is emphasized. Four to six ad libitum fed growing rabbits are necessary to obtain sufficiently accurate results. The reliability of the estimation of the feeds' energy content, based on the table values of the ingredients, is discussed. The importance of using recently and properly determined data is stressed. Multiple regression equations, based on the results of the chemical analysis of feeds, explain 85-90% of the variability of DE content. The first attempts with in vitro techniques are encouraging.

Animal Feed↗
PubMed1990

The effect of animal fat and vegetable oil supplementation of feeds of different energy concentration upon the digestibility of nutrients and some blood parameters in rabbits.

The effect of mixed animal fat and sunflower oil supplementation (5%) of a feed of medium (12.02 MJ DE/kg) and low (8.54 MJ DE/kg) energy concentration upon the digestibility of nutrients and on some blood parameters was investigated. The ether-extractable content of feed and faecal samples was determined by diethyl ether extraction (after Soxhlet) and the total (true) fat level was measured by the method of Stoldt (1952), viz. petroleum ether extraction of samples pretreated with 4 N HCl. In the majority of cases the voluntary feed intake decreased after the addition of animal fat or vegetable oil. Mixed animal fat supplementation significantly (by 5 and 11 units) improved the digestibility of the ether extract if added to either of the basal diets. The digestibility of crude fibre and N-free extract increased only in the case of the basal diet of low energy concentration (by 4 and 7 units, respectively). Sunflower oil addition produced changes of the same tendency (9, 9, 28 and 5 units). The digestibility of the crude protein was practically not altered by either supplementation. The total fat content of feed and faecal samples proved to be higher than that of the ether extract, on an average by 27 and 100%, respectively. Consequently, the digestibility coefficients of the total fat are by 10 units lower than those of the ether extract. The DE concentrations, calculated by means of the digestible ether extract or the total fat content, differed significantly only between the basal diets. The addition of mixed animal fat to either of the basal diets increased the blood concentration of total lipids (by 18 and 32%, respectively), while that of sunflower oil did not cause such an alteration. Neither fat nor oil supplementation had an effect on the cholesterol level of the blood plasma. Both the animal fat and the vegetable oil surplus modified the fatty acid composition of blood lipids. It was characteristic of each treatment that the proportion of polyunsaturated fatty acids increased.

Animal Feed↗
PubMed1990

The effect of tannin treatment and subsequent urea supplementation of sunflower meal on the in vitro digestibility of its crude protein for ruminants.

The effect of tannin treatment (TSFM) and subsequent urea supplementation (TSFM + U) on the digestibility of sunflower meal (SFM) protein was studied by means of a three-phase laboratory method simulating the ruminant's digestion. Under the influence of the ruminal fluid 67% of SFM protein, 62% of TSFM protein and 58% of TSFM + U protein was degraded. The pepsin and pancreatin digestibilities of rumen undegraded protein (UDP) were as follow: 58.2% (SFM), 56.6% (TSFM), 43.3% (TSFM + U), and 22.0% (SFM), 28.8% (TSFM), 17.5% (TSFM + U), respectively. The four fractions (dissolved protein, oligopeptides, amino acids, and ammonia) of rumen degradable protein (DP) were also determined: 85 to 92% of rumen degradable protein was recovered in these four fractions. Owing to tannic acid treatment the quantity of dissolved protein decreased and that of oligopeptides increased.

Animal Feed↗
PubMed1989

[Isolation of Corynebacterium JK from the blood of neutropenic patients with septic diseases].

The authors report on the isolation of Corynebacterium JK from the blood of two neutropenic patients with hematological malignancy. In Hungary this paper is the first to discuss the microbiology of the organism and clinical manifestations of the group JK Coryneform infection. The clinical significance of this organism in the nosocomial infections of compromised patients and the association of the infection with the use of plastic devices has been emphasized. The sensitivity of the multiresistant coryneform bacterium to vancomycin may help to make the microbiological diagnosis and to select the drug for therapy.

Adult↗
PubMed1989

Cytochemical maturation index for the prognostication of adult acute nonlymphocytic leukaemia.

76 adult acute nonlymphocytic leukaemias (ANLL) were characterized by cytochemical markers and placed in a coordinate system. The position of each ANLL was determined on the basis of the peroxidase and nonspecific esterase reactivity of the blast cells. This classification numerically identifies the maturation tendency and heterogeneity of individual ANLL cases according to its position in the coordinate system. 49 ANLL patients were treated with TAD protocol and the response rate seemed to be in a conspicous correlation with the position of the individual ANLL cases in the modified arrangement. Cases exhibiting a strong peroxidase maturation, i.e. the cytochemical maturation index being 80% or more had a considerable higher complete remission rate and longer duration of remission than those with low (less than 80%) maturation index. Age profoundly influenced even this figure.

Adult↗
PubMed1989

Effect of T-2 toxin on feed intake, digestion and pathology of rabbits.

Feed containing sublethal T-2 toxin concentrations (12.5 and 25 ppm) was fed to adult rabbits. The animals ate 60-70% less toxin-containing food. The dry matter content of their feces decreased significantly (on an average by 10%). The nutrient digestibility of the feed containing 12.5 ppm T-2 toxin, was increased by 2-6% and that of the 25 ppm T-2 toxin level decreased by 4-11% as compared to the control values. The rabbits showed emaciation, subacute catarrhal gastritis, necrosis of the lymphoid cells of the intestinal mucosa, depletion and necrosis in the lymphoid follicles of the ampulla ilei, spleen and lymph nodes. Necrosis of the cells of mononuclear phagocyte system and myeloid hemacytogenesis was characteristic. The toxin concentration of feces, cecotroph and urine was proportional to intake.

Animals↗
PubMed1989

In vitro evaluation of protein degradability in the rumen and digestibility of undegraded protein.

A three-phase laboratory procedure suitable for predicting protein degradability in the rumen and digestibility of undegraded protein is reported. In the first phase the feed was incubated with starch and buffered rumen fluid. In the incubation mixture the viability of protease-active bacteria was checked by anaerobic culturing, whereas changes in protease activity were monitored by azocasein degradation. In the second and third phase rumen undegradable protein (UDP) was digested with pepsin and pancreatin, respectively. The measurements showed that 63.2, 5.2 and 4.7% of the crude protein of green lucerne was decomposed by rumen fluid, pepsin and pancreatin, respectively. Degradability of the crude protein of extracted sunflower meal was 68.3, 17.7 and 5.5% in the three phases, respectively. Repeated determination yielded crude protein degradabilities of 66.7, 27.1 and 5.1% for the three phases, respectively.

Animal Feed↗
PubMed1989

Acute toxicological experiment of T-2 toxin in rabbits.

Rabbits were treated with a single oral dose (1, 2, 4, 6, 8, 10 or 15 mg/kg body mass) of T-2 fusariotoxin. Doses of 4 mg or higher killed the animals in 24 to 48 h. As opposed to the controls, in the treated rabbits gross pathological and histopathological examinations revealed acute catarrhal gastroenteritis, necrosis of lymphoid cells of the gastrointestinal mucosa, centrolobular dystrophy of the liver, necrosis of cells of the mononuclear phagocyte system (MPS) in the liver, tubulonephrosis, focal dystrophy of the adrenal cortex, lymphocyte depletion involving both T- and B-cell-dependent zones of the lymphoid organs (spleen, lymph, ampulla ilei), and depletion and necrosis of the myelopoietic cell colonies of the bone marrow. Similar but milder changes were observed in surviving rabbits exsanguinated 48 h after treatment. In addition to the direct damage done to the digestive tract mucosa and liver, the toxin severely damaged the cells participating in humoral and cell-mediated immunity and in the local defence of the intestinal mucosa, and markedly impaired phagocytosis and granulocytopoiesis. In another experiment rabbits were given oral doses of 2 mg/kg body mass T-2 toxin daily for several days. One rabbit was killed by bleeding every day. In rabbits killed beyond day 7 there was subacute catarrhal gastritis, emaciation, and hypertrophy of the adrenal cortex.

Animals↗
PubMed1989

Restricted feed intake influences thyroid hormone production and peripheral deiodination in chickens.

Thyroid hormones are of major importance in determining metabolic state both in mammals and in birds. Whether or not feed intake itself has an autoregulatory role in adjusting the setpoint of thyroid hormone activity is yet not well understood. The present work investigates the effects of restricted feed intake on the serum levels and on the peripheral metabolism of thyroid hormones. Also, the sensitivity of the pituitary-thyroid axis was looked at by means of thyrotropin releasing hormone provocation test. Several groups of Hunniahybrid chickens aged 2-6 weeks were used. Restriction was made by providing the experimental animals 70 and 85 percent of the amount of food consumed in the ad libitum fed control group. It was found that feed restriction lowers circulating concentration of triiodothyronine probably by inhibiting the activity of liver deiodinase and also decreases the sensitivity of the pituitary-thyroid axis meaning that both central and peripheral regulatory mechanisms of thyroid economy are affected by feed restriction.

Animals↗
PubMed1989

Effect of heat treatment and subsequent urea supplementation of sunflower meal on the in vitro ruminal degradability of crude protein content and its postruminal digestibility.

A three-phase laboratory procedure was used for predicting the degradability of the protein of extracted sunflower meal before (SFM) and after heat treatment (HSFM). The rumen fluid degraded 69.1% and 67.1% of the SFM and HSFM protein, respectively. The digestibility values of rumen undegraded protein (UDP) were 57.3% (SFM) and 57.0% (HSFM) with pepsin and 17.6% (SFM) and 15.9% (HSFM) with pancreatin. Urea supplementation practically did not alter the rumen degradability of HSFM protein, while the pepsin digestibility of UDP decreased to 47.2%. Four fractions (NH3, dissolved amino acids, oligopeptides and proteins) of rumen degradable crude protein (RDP) were also determined in vitro: 81 to 92% of the degraded crude protein was found in the fractions tested. Heat treatment reduced free NH3 content but did not alter the other three fractions. Urea supplementation decreased the quantity of NH3, peptides and proteins as well.

Animal Feed↗
PubMed1989

Recent findings and future perspectives of digestive physiology in rabbits: a review.

The rabbit is one of the most common laboratory animals. Consequently, there is an enormous number of publications concerning its physiology. The present review is restricted to a special field and deals with the new findings of the rabbit's digestive physiology. For the sake of better intelligibility the most important antecedents are also given. First the particularities of feed intake regulation are demonstrated. The strong control mechanism can be disturbed by feed antinutritives, mycotoxins and high environmental temperature. Caecotrophy depends on three main factors: stimulation of rectal mechanoreceptors, perception of the specific odour of the soft faeces, and the inner motive determined by the blood level of metabolites and hormones. The species characteristics of proportions, pH conditions, microflora and -fauna of the rabbit's digestive tract are given. The digestion and absorption of dietary nutrients are discussed. Special sections deal with the sorption of electrolytes and VFAs in the gut, the caecal digestive process, the formation of hard and soft faeces and the role of the caecotrophy in stomachal carbohydrate degradation. A relatively new area, i.e. the development and maturation of the gastrointestinal tract, is also shown. The postnatal evolution of digestive enzyme activity is also summarized. The main endogenic factors (breed, sex, age, stress, caecotrophy, presence of hairball in the stomach) influencing the digestion of dietary nutrients are also described. The probable future trends are given, too.

Animals↗
PubMed1988

Bone scanning and radiography in the evaluation of patients with malignant lymphoma.

The diagnostic value of bone scintigraphy and radiography in the detection of lymphomatous bone involvement, and the role of bone scintigraphy in the evaluation of lymphomatous bone marrow involvement, were investigated in 41 patients with malignant lymphoma. 10 patients had lymphomatous bone involvement. Whereas scintigraphy detected all the 10 cases, radiography was false negative in 2 cases. The lytic bone lesions on radiography were in most cases not detected by scintigraphy. Scintigraphy is insensitive for the detection of early bone marrow metastases. The simultaneous use of bone scanning and x-ray, however, seems to be helpful in the detection of lymphomatous bone involvement and consequently in the clinical management of patients with malignant lymphoma.

Adolescent↗