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S Faine

Publications and source records attributed to S Faine.

89 records · Page 5Linked to original sources
PubMed1973

Independent antibiotic actions of hetacillin and ampicillin revealed by fast methods.

Fast methods for measuring inhibition of growth of Escherichia coli or Staphylococcus aureus by hetacillin or ampicillin showed that the rate of inhibition was a function of the antibiotic concentration. When ampicillin concentrations were measured rapidly, hetacillin was shown to have a half-life of 20 min at 37 C and pH 6.7. Hetacillin was much less susceptible to penicillinase (beta-lactamase) than was ampicillin. An inhibitory effect of hetacillin was shown in the presence of beta-lactamase, which destroyed ampicillin activity very rapidly.

Ampicillin↗
PubMed1972

Research needs in leptospirosis.

Leptospirosis is a widespread infection of man and animals, and locally it assumes considerable importance as a public health and economic problem. It is an important occupational infection among persons working with animals or in environments that may be contaminated by infected animals. In recent years, considerable attention has been devoted to this infection but efforts to control and eliminate it, especially from natural foci, are hindered by gaps in our knowledge. This memorandum is a review of recent progress and current problems in leptospirosis research, with special reference to taxonomy, epidemiological methods, and control measures. Certain aspects of the basic biology of leptospires that are relevant to these topics are also discussed.

Agglutination↗
PubMed1970

Electron-microscopic evidence for reactions of axial filaments of Leptospira with IgM and IgG antibodies.

Serological identifications of structural components of leptospires have not previously been reported although morphological and antigenic studies have been carried out extensively.The antigenic activity of isolated axial filaments was characterized by direct electron-microscopic visualization of immune precipitates with fractionated antisera. The precipitates were composed of axial filament with IgM or IgG antibodies attached.The axial filament is the first structural component of leptospires to be identified serologically. Knowledge of the pattern of cross-reactivity of this axial filament antigen among various serotypes may be of value in helping to establish a new system of classification based on specific antigens rather than the current system using reciprocal agglutinin-absorption tests, which measures the resultant of many antigens.

Journal Article↗
PubMed1970

Antileptospiral agglutinins produced in rabbits.

Agglutinating antibodies produced in an adult rabbit in response to intravenous injections of live Leptospira sp. (serotype biflexa, strain Waz), consisted of a 19S component (present at day 6 after inoculation) and a 7S component (present at day 6 after inoculation). Both 19S and 7S antibodies persisted in the serum until the conclusion of the observations (day 216). The 19S antibody was completely destroyed by 2-mercaptoethanol but the 7S antibody was only partially sensitive to 2-mercaptoethanol.The serum proteins present in the 19S and 7S serum fractions were determined by immunoelectrophoresis and cellulose acetate electrophoresis. Elution of the serum components from cellulose acetate membranes directly confirmed that the beta-migrating IgM was the 19S serum component with leptospiral agglutinating activity and that gamma-migrating IgG was the 7S serum component with antileptospiral agglutinating activity.

Animals↗
PubMed1968

Natural antibody in mammalian serum reacting with an antigen in some leptospires.

Serum from normal mammals agglutinated and immobilized nonpathogenic Leptospira biflexa and agglutinated avirulent lines of pathogenic serotypes L. icterohaemorrhagiae and L. zanoni. Virulent lines of L. icterohaemorrhagiae and L. zanoni were not affected, nor were any of three strains of L. pomona, one of which was avirulent. The active principle in serum was a beta-macroglobulin which was heat-labile and reduced by 2-mercaptoethanol, and acted in conjunction with complement and lysozyme; it was absorbable from serum by Formalin-treated susceptible leptospires. The Formalin-stable receptor antigen, named "Z antigen," is associated with virulence rather than pathogenicity, but may not be a determinant of virulence.

Animals↗
PubMedDate not supplied

Musical chairs.

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Bed Occupancy↗