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PubMed1999

DNA ploidy of liver biopsies from patients with liver cirrhosis and hepatocellular carcinoma: a flow cytometric analysis.

Flow cytometric DNA analysis was used to assess cellular kinetics of needle liver biopsies from patients with liver cirrhosis and hepatocellular carcinoma (HCC). An abnormal DNA content was shown in 44.5% of liver cirrhosis cases and in 78.6% of tumor sites. The number of proliferating cells (S + G2M%) was significantly increased in cirrhotic liver (P < 0.05). Dysplasia was found in 66% of cirrhotic specimens. All negative dysplasia specimens showed a diploid pattern while 69% of positive dysplastic specimens were aneuploid (P < 0.001). In conclusion, cell proliferation, aneuploidy and liver cell dysplasia are important indicators in liver cirrhosis for the development of HCC.

Adult↗

PubMed1997

Brucellosis in fish.

Brucellosis is a zoonotic infectious disease that represents serious economic and health problems. It has been extensively studied in different species of livestock but literary references to fish brucellosis are scarce. Therefore, the purpose of the research hereby presented was to investigate the serologic-bacteriologic response of Nile catfish to experimental infection with brucellosis. Thirty predatory catfishes were used. They were divided into two groups. The first was subjected to s. c. infection with Brucella melitensis biovar 3, the other to s. c. application of physiological solution. Three fishes from each group were killed once a week within 5 weeks to subject them to serologic-bacteriologic examinations. It was found that brucella antibodies were detected in the blood of infected fishes after 7 days post-inoculation. The antibody titre was maintained throughout the period of observation. The organism was also recovered from visceral organs of infected fishes. On the other hand, none of control fishes showed any serologic or bacteriologic response. These results suggest that fish could be considered as a suspectable species to brucellosis. Subsequently, further studies are needed to clarify the possible role of fish in the disease transmission.

Animals↗