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Biomedical subjects

S F Patten

Publications and source records attributed to S F Patten.

At least 37 records · Page 2Linked to original sources
PubMed1977

Imaging systems for correlation of false alarms in flow.

False alarms, arising from a variety of sources, are the greatest remaining obstacle to development of an automated prescreening system for gynecologic cytology. This paper describes two correlation systems under development at the University of Rochester and discusses their utilization in the study of false alarms in slit-scan cytofluorometry. Both systems permit imaging of objects in flow and correlation between images and corresponding slit-scan contours. Correlation systems will permit a detailed study of false alarm causes and aid in the search for new features to assist in their recognition.

Autoanalysis↗
PubMed1976

Histopathologic spectrum of benign proliferative and intraepithelial neoplastic reactions of the uterine cervix.

A working model for the histogenesis of carcinoma of the uterine cervix is summarized in Table I. The inclusion of squamous metaplasia in this chart does not imply that this reaction falls into the spectrum of cervical neoplasia or is necessarily an antecedent to neoplasia. It does simply imply that the carcinogenic event or events apparently occur in or involve an epithelium that is indistinguishable from squamous metaplasia. The chart intentionally implies that the lesions mentioned are not separate diseases but arbitrary points in the spectrum of cervical neoplasia. It must be emphasized that one stage does not necessarily progress to the next and that at any stage up to indisputable cancer the changes may regress, persist or progress. The careful evaluation of histologic material from the uterine cervix will permit the pathologist to exclude those epithelial abnormalities which are not a part of the spectrum of cervical neoplasia and allow him or her to place the patient with cervical neoplasia at the proper stage in the development of the process. With this information the clinician can then intelligently plan appropriate therapy.

Carcinoma in Situ↗
PubMedDate not supplied

AutoPap 300 QC system scoring of cervical smears without "epithelial cell abnormalities".

OBJECTIVE: To examine four morphologic features other than epithelial abnormality to identify if they are associated with a high quality control (QC) score with the AutoPap 300 QC system. STUDY DESIGN: A total of 180 slides (140 with a high QC score and 40 with a low QC score [the control group]) were manually reviewed, and four morphologic features were assessed while blind to the QC score, as follows: adequacy, epithelial fragments and clumps, cytohormonal pattern, and diagnostic categories within normal limits and benign cellular changes (BCC). RESULTS: Both atrophy and a diagnosis of BCC were associated with a high QC score at a statistically significant level. CONCLUSION: Certain characteristics of the slide population submitted for AutoPap QC review could have an impact on the overall laboratory workload (QC review rate). There are opportunities to optimize the workload of the laboratory when using AutoPap for QC selection.

Adult↗
PubMedDate not supplied

Enhancing the performance of the AutoPap 300 QC system with optimal staining and presentation of cervical smears.

OBJECTIVE: To optimize the staining and presentation of slides for the AutoPap 300 QC System, an automated cytology screening system which examines conventionally prepared cervical smears, and to assess subsequent scanning and scoring rates and compare them to those of laboratories not adopting these changes. STUDY DESIGN: In this study, procedures were developed for optimal presentation and staining of slides for the AutoPap System in response to observations made in preclinical trials. Three thousand eight hundred fifty-five slides were then submitted to the device for analysis in a prospective, blind, clinical evaluation study. The scanning and scoring rates were compared to those of a cohort of 12,525 slides that were analyzed in other laboratories which had not adopted these procedures. RESULTS: Two hundred forty (6.2%) slides failed scanning due to physical defects. An additional 70 slides (2.0% of scanned slides) did not complete scoring due to staining limitations. The laboratories in the clinical trials that had not adopted these preanalytic method changes had higher scanning failure rates due to physical limitations (15.0%) and incomplete scoring rates due to staining limitations (6.2%). CONCLUSION: The present study demonstrated that the performance of the AutoPap 300 is enhanced by meticulous attention to preanalytic staining and presentation procedures.

Automation↗
PubMedDate not supplied

AutoPap system performance in screening for low prevalence and small cell abnormalities.

OBJECTIVE: To summarize the design principles of the AutoPap System evaluation score by evaluating slides having a low prevalence of abnormal cells and small cell abnormalities and assessing the evaluation score as a diagnostic tool. STUDY DESIGN: Data from two clinical studies conducted using the AutoPap System and data obtained from the evaluation score training slides were analyzed to demonstrate the effectiveness of the evaluation score. The clinical studies included a prospective, intended-use study involving approximately 13,000 slides and a comprehensive sensitivity study using approximately 1,200 slides from five laboratories. The evaluation score training set consisted of 4,174 slides from 10 laboratories. RESULTS: The robust design of the AutoPap evaluation score was demonstrated by similar detection capabilities and sensitivities to slides having either a low or high prevalence of abnormal cells. No significant difference in performance was detected between the small cell slides and the comparison groups of carcinoma in situ and invasive squamous carcinoma having normal-sized abnormal cells. In addition, the evaluation scores corresponded well to the diagnostic severity of the slides.

Algorithms↗
PubMedDate not supplied

Sensitivity of the AutoPap 300 QC System for cervical cytologic abnormalities. Biopsy data confirmation.

OBJECTIVE: To assess the AutoPap 300 QC System's sensitivity to cases of high grade squamous intraepithelial cervical lesion and invasive cervical cancer, a comparison of outcome was made in cases that had surgical biopsy confirmation. STUDY DESIGN: The sensitivity of the device to biopsy-confirmed diagnostic categories at the level of high grade squamous intraepithelial lesion (HSIL) and greater was calculated. At two of six clinical trial sites, biopsy data were available and were compared to device scores indicating whether a case would or would not be selected for 10% or 20% quality control rescreening. RESULTS: In 86 biopsy-positive cases that had a cytologic diagnosis of HSIL or greater, the device indicated inclusion in the 10% or 20% review fraction for 66 slides (77%) and 74 (86%), respectively. This figure indicated an approximately eightfold improvement in the ability of the device to include such cases for quality control rescreening when compared to a 10% random case selection process. CONCLUSION: These data showed that the sensitivity of the AutoPap in identifying cases of HSIL and greater was similar to that previously calculated for these diagnostic categories in clinical trials, when calculated on the basis of an alternate or "higher" standard of tissue confirmation.

Biopsy↗
PubMedDate not supplied

Fibroxanthosarcoma of the uterine cervix: cytopathologic and histopathologic manifestations.

A case of fibroxanthosarcoma of the uterine cervix is reported with its cytopathologic manifestations. The cellular features of two cell populations characterized by atypical cells of fibroblastic and histiocytic types, suggests the correct diagnosis. The possibility of uterine sarcoma must be considered in the differential diagnosis of bizarre and unusual cytologic findings.

Adult↗
PubMedDate not supplied

Plastic-embedded semi-thin sections of fine needle aspiration biopsies with dibasic staining. Diagnostic and didactic applications.

The diagnostic and didactic utility of plastic-embedded semi-thin sections of fine needle aspiration biopsies is presented using a case-study approach. The Spurr epoxy semi-thin sections were stained with a newly developed sequential basic fuchsin-methylene blue stain, which gives hematoxylin-and-eosin-like staining and simultaneously substitutes for a wide variety of special stains. The informational content of the sections can approach that of electron microscopy. The use of a direct off-the-slide "pop-off" technique in preparing the plastic-embedded sections allows for a direct comparison between similar groups of cells embedded in plastic and present on the routine aspiration slides; retrospective analysis can discern subtle, previously unrecognized morphologic features in the alcohol-fixed, Papanicolaou-stained slides. The limitations of this comparative approach, however, become manifest when the effects of alcohol fixation on cells are directly compared in plastic and at the ultrastructural level to aldehyde fixation.

Adenoma↗