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Biomedical subjects

S F Gould

Publications and source records attributed to S F Gould.

5 recordsLinked to original sources
PubMed1979

Fine structure of fetal human testis and epididymis.

Observations on the structure of the human fetal testis and epididymis at 16 weeks' gestation have been made with the light and electron microscope. The fetal testis is organized into solid cords surrounded by a well-defined cellular investment. The basement membrane is multilaminated with a highly redundant basal lamina. The germ cells rest on thin processes from adjacent Sertoli cells. Intercellular bridges between centrally located germ cells have been observed. The epididymis is remarkably well developed. The tall pseudostratified epithelium lines a discrete duct with a patent lumen. Stereocilia and cilia are seen on apical surface of the principal cells.

Cytoplasm↗
PubMed1978

Malachite green: applications in electron microscopy.

Incorporation of malachite green into a glutaraldehyde fixative results in enhanced staining of a number of cellular elements. Ribosomes and myofilaments exhibit increased electron density, but cell membranes generally are not stained. In certain tissues, lipid inclusions are uniformly and heavily stained. Other populations of lipid droplets exhibit differential affinity for malachite green, facilitating their division into subclasses. In addition to its function as a dye, malachite green has previously been reported to stabilize lipid elements soluble in aqueous glutaraldehyde. Such a component was observed in the stroma of uterine endometrium. The variety of cell components which exhibit increased contrast after preparation with malachite green suggests that this technique may find widespread application in fine structure studies.

Adrenal Cortex↗
PubMed1975

The localisation of bovine sperm hyaluronidase.

Hyaluronidase is a component of the acrosome of bovine spermatozoa. Utilizing fluorescein and peroxidase-labelled antibodies, the enzyme has been localised at both the light and electron microscopic levels. Antibodies against purified bovine testicular hyaluronidase were prepared in New Zealand white rabbits. Immunodiffusion, immunoelectrophoresis, and immuno-inhibition studies of the antiserum demonstrated the presence of an antibody specific for bovine sperm hyaluronidase with no cross reactivity to other sperm-associated antigens. Fluorescence microscopy of ejaculated bovine spermatozoa show the enzyme to be limited to the anterior portion of the acrosome with a sharp termination at the rostral border of the equatorial segment of the acrosome. Light microscopic studies with peroxidase-labelled antiserum were similar to the fluorescent findings. Fine structure studies revealed reaction product predominately associated with the matrix of the acrosome cap. Within the acrosome, more intense localisation was observed in the apical densities, while no product was visualised within the equatorial segment. These findings are compatible with the hypothesis that the enzyme functions to facilitate the passage of spermatozoa between cells.

Acrosome↗