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S F Dealler

Publications and source records attributed to S F Dealler.

31 records · Page 2Linked to original sources
PubMed1989

Reliable five-minute test strip method for identification of Streptococcus pyogenes.

A novel and rapid method (Strep Strip, Lab M) for identification of Streptococcus pyogenes was evaluated. The method combines the established test for pyroglutamyl aminopeptidase (PYR) with a rapid chromogenic test for beta-glucosidase on a paper strip. The test was evaluated with 274 clinical isolates and 237 culture collection isolates of beta-haemolytic streptococci. Streptococcus pyogenes was identified with 100% specificity. Six isolates identified as Lancefield group A and which might therefore be assumed to be Streptococcus pyogenes were shown by the test strip method not to be this species. The beta-glucosidase test on the test strip allowed differentiation between enterococci and Streptococcus pyogenes (both PYR positive) in all cases. The test strip method represents an economical and accurate method for identification of Streptococcus pyogenes in clinical material.

Aminopeptidases↗
PubMed1989

Purple urine bags.

Purple urine drainage bags were found in 7 of 71 chronically catheterized elderly women. The purple staining of the bags is due to a violet discoloration (indirubin) of the plastic of the catheter bag and fine blue crystals of indigo in the urine. The colors are formed from the substrate indoxyl sulfate (indican) and all 7 patients had bacteria in the urine that would produce blue colonies on agar enriched with the urine (filter sterilized) of the patients involved. Organisms identified were Providencia or Klebsiella species. Indican excretion was higher in patients with purple urinary catheter bags than in controls.

Aged↗
PubMed1989

Bacterial contamination of intraocular lenses: the source of the bacteria.

In order to investigate potential sources for the bacterial contamination of intraocular lenses, specimens were taken for culture from a variety of sites. A swab from the lid margin, the operating room air, an intraocular lens which was allowed to rest on the eye, and the sodium hyaluronate were cultured during routine intercapsular lens implant surgery on 31 patients. Positive cultures were obtained from six intraocular lenses. Subtyping by three independent methods showed that isolates from five of these lenses were indistinguishable from bacteria cultured from the air. In the case of the sixth lens a bacterial isolate from the lid margin matched with that from the lens. Staphylococcus epidermidis was cultured from four lenses; Staphylococcus haemolyticus was also isolated from one of these four lenses and the remaining two lenses produced isolates of Staphylococcus aureus.

Air Microbiology↗
PubMed1989

Identification of Branhamella catarrhalis in 2.5 min with an indoxyl butyrate strip test.

Branhamella catarrhalis, an occasional cause of human respiratory infections, unlike most other members of the family Neisseriaceae, produces a butyrate esterase. This is capable of breaking the ester linkage between butyryl groups and carrier molecules. B. caviae and B. ovis, which are rarely encountered in pathological specimens, also produce butyrate esterase. This property can be used as a rapid test in the identification of B. catarrhalis. The recently reported rapid test for butyrate esterase relies on the release of methylumbelliferate, which can be detected only by using UV light after 5 min of incubation. In the rapid test described here, indoxyl is liberated from indoxyl butyrate by butyrate esterase and spontaneously forms indigo in the presence of oxygen. B. catarrhalis can be distinguished from other oxidase-positive, gram-negative cocci after 2.5 min by inoculating the organism onto a filter paper strip containing this compound.

Carboxylic Ester Hydrolases↗
PubMed1988

Enzymatic degradation of urinary indoxyl sulfate by Providencia stuartii and Klebsiella pneumoniae causes the purple urine bag syndrome.

The etiology of the purple urine bag syndrome (PUBS), in which the urinary catheter bag of some elderly patients develops intense purple coloration, was studied. The purple was found to be a mixture of indirubin dissolved in the plastic and indigo on its surface. Six patients with PUBS were studied, and Providencia stuartii was isolated from the urine of five and Klebsiella pneumoniae was isolated from the urine of one. These strains produced indigo in 7.9 mM indoxyl sulfate-containing agar. One hundred and fifty isolates of 41 species of bacteria were tested for their ability to produce indigo on agar containing indoxyl sulfate, but only 17 of 27 strains of P. stuartii, a single strain of Klebsiella pneumoniae, and Enterobacter agglomerans were positive. All of the indigo-producing bacteria had an indoxyl phosphatase with a pI of 6.4. This enzyme also possessed indoxyl sulfatase activity and was not present in strains that were unable to produce indigo from indoxyl sulfate. We conclude that PUBS results from the decomposition of urinary indoxyl sulfate to indigo and indirubin by bacteria (notably P. stuartii). As elderly catheterized patients often have high urinary indoxyl sulfate levels and colonization of their urinary tract with P. stuartii, the condition is most commonly seen in them.

Bacteriuria↗
PubMed1981

Electrical phenomena associated with bones and fractures and the therapeutic use of electricity in fracture healing.

Living bones have small electrical potentials on their surfaces, the magnitudes of which change only slightly with the reaching of adulthood. When using a limb stresses are put on the bones inside, and these create piezo-electric potentials which may themselves cause bone growth along the lines of stress, hence making the bone stronger. Voltages also appear at fracture sites and may be important in causing the bone to heal its wound. Fractures that have not healed properly can be stimulated into repair by the passage of small electric currents through them. The factors involved in these processes are discussed.

Biomechanical Phenomena↗