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Biomedical subjects

S E Olsson

Publications and source records attributed to S E Olsson.

At least 19 recordsLinked to original sources

Effects of limited food consumption on the incidence of hip dysplasia in growing dogs.

Forty-eight 8-week-old Labrador Retrievers were allotted to 2 groups of 24 dogs each; 1 group was fed ad libitum and the other group was given 25% less of the same feed until the dogs were 2 years old. Radiography of the hip joints was done when the dogs were 30, 42, 54, 78, and 104 weeks old. Subluxation was measured by the Norberg angle on radiographs made with the dog in the standard (extended limb) position. Independent of age at which the radiography was done, there was less subluxation of the femoral heads in the limit-fed dogs. Using the Swedish method of hip joint evaluation on the same radiographs, it was found that fewer dogs on limited food intake had signs of hip dysplasia. Radiographs done when dogs were 2 years old, for all the methods used (Norberg angle in standard and frog-limb position, the Orthopedic Foundation for Animals [OFA] score, and the Swedish score), revealed less hip dysplasia (less joint subluxation and less degenerative joint disease) in the limit-fed dogs. Using the OFA method, 7 of the 24 limit-fed dogs and 16 of the 24 ad libitum-fed dogs were diagnosed as having hip dysplasia. Similarly, using the Swedish method, 5 of the 24 limit-fed dogs and 18 of the 24 ad libitum-fed dogs were diagnosed as having hip dysplasia. The food-intake-related differences were significant both for the OFA score and for the Swedish score.(ABSTRACT TRUNCATED AT 250 WORDS)

Animal Feed

Proteoglycan alterations during developing experimental osteoarthritis in a novel hip joint model.

Degenerative hip joint disease was induced in dogs by extra-articular surgery that created a condition that mimics hip dysplasia. Decreased acetabular coverage of the femoral head gave altered mechanical load, with ensuing cartilage degeneration. For comparison, degenerative knee joint disease was induced in other dogs by transection of the anterior cruciate ligament of the knee. The femoral head articular cartilage showed macroscopic signs of degeneration within a month. No macroscopical changes of synovitis were present. Chemical analysis of cartilage samples showed loss of proteoglycans. Guanidine hydrochloride extracts of the cartilage contained proteoglycan fragments that could be separated by equilibrium density gradient centrifugation in cesium chloride. The data indicate that proteoglycans are fragmented by proteolytic cleavage and lost from the cartilage. The proteoglycans remaining in the tissue are smaller and have lost the ability to aggregate with hyaluronic acid. Similarly, in experimental knee joint osteoarthritis, the proteoglycan content of the cartilage decreased. The structural changes of those proteoglycans remaining were of a different nature, with no changes in proteoglycan size or aggregation properties, possibly indicating that both degradation and repair took place in the knee articular cartilage and/or that fragments were rapidly lost from the tissue. This may follow from different surgical procedures, only the one used for the hip joint being extra-articular, or from the different anatomy and physiology of the hip joint and the knee joint.

Animals

Two-year comparative trial of the Gyne T* 380 Slimline and Gyne T* 380 intrauterine copper devices.

In a randomized trial of the Gyne T* 380 Slimline, the-two year pregnancy rate was 0.3 per 100 and the continuation rate was 65 per 100. These rates did not differ statistically from those of the standard Gyne T* 380, nor did other performance parameters differ between devices by the log-rank test. Women under age 30 had lower continuation rates using either device than did women age 30 or older at admission because of removals for planned pregnancy. Conception rates among those who desired pregnancy were 35 per 100 at one month and 86 per 100 at one year.

Adult

Detection of Chlamydia trachomatis in urinary samples from women.

With a mean age of 21 years 197 women at risk for an infection with Chlamydia trachomatis (CT) had a urinary sample (20 ml first-void urine, minimum 4 hours from prior mictuation) analysed with an enzyme immunoassay (IDEIA-III) for the detection of CT. They also had samples taken from both cervix and urethra for cultivation on McCoy's cells and testing with an enzyme immunoassay (Chlamydiazyme), plus verification of positive samples in the enzyme immunoassay (EIA) with monoclonal antibodies against CT. The urethral samples were compared against the urinary samples with regard to sensitivity and specificity in detecting CT. Women with a positive culture for CT and/or a positive verified EIA from either the cervix or the urethra, were regarded as "true" infections with CT. The prevalence of CT was 12.2%. The urinary EIA sample had a sensitivity of 84% whereas the urethral EIA sample had a sensitivity of 57%. The specificity was 98% and 100% for the urinary samples, and the urethral samples respectively. It is concluded that the urinary sample is superior to the urethral sample, and that the urinary sample could be used for screening programs, to detect CT among women.

Adolescent

Sexual behavior and sexually transmitted diseases among Swedish university students.

Students at Uppsala University, Sweden, were interviewed about their sexual behavior, their knowledge of and attitudes to sexually transmitted diseases (STDs) and related issues. The purpose of the study was to ascertain trends in behavior and attitudes as an indication of the likely risk of the spread of STDs, in particular AIDS, among students. Results revealed that change of partner was commonplace. A significant number of students had suffered from an STD. Students' claims that their own sexual practices, and those of others, had changed as a result of the AIDS epidemic, proved to be unsubstantiated in their behavior. Although contraceptive use was high, condom use with change of partner was infrequent. Alcohol played a significant role in impairing judgement. Despite a reasonably sound knowledge of STDs, the students exhibited a high degree of risk-taking behavior. The study has highlighted the dichotomy between knowledge and practice in student sexual behavior. Notable was the lack of perception among university students of their own risk of contracting STDs.

Acquired Immunodeficiency Syndrome

Screening for Chlamydia trachomatis infection in women and aspects of the laboratory diagnostics.

Nine hundred and thirty-three women who attended consecutively a gynecological clinic for various symptoms, for abortion, or for contraceptives, were screened for genital Chlamydia trachomatis infection. A total of 95 (10.2%) women were found infected. Of women below 25 years of age, 13.4% were infected, as compared with 4.9% over 25. Women with symptoms of genital infection were not over-represented in the infected population. Enzyme immunoassays (EIA) verified with a direct fluorescence antigen test were evaluated and compared with culture controls. Reactive samples detected using EIA were regarded as true positive if they were also positive according to the verification test. The sensitivity of the verified EIA test was 91%, the specificity 100%, the positive predictive value 100% and the negative predictive value was 99%. The sensitivity of the culture was 90%. Low age is the most predictable risk factor for genital Chlamydia trachomatis infection. Compared with cultures, EIA verified with a direct fluorescence antigen test is a rapid and effective method for clinical use.

Adolescent

A randomized trial of the Gyne T 380 and Gyne T 380 Slimline Intrauterine Copper devices.

To facilitate manufacture and insertion of the Gyne T 380 IUD, design changes were instituted. Copper collars were seated flush at the ends of the horizontal crossbar of the device. A randomized study of the Gyne T 380 Slimline, the new design, was undertaken in comparison with the standard Gyne T 380. A total of 996 women were enrolled, with 698 Slimline insertions and 298 of the standard Gyne T. No statistically significant difference in ease of insertion or in performance was detected between the models. At one year, the pregnancy rate of each model was below 0.5 per 100 and the continuation rate was 79-80 per 100. Pelvic inflammatory disease or endometritis was found in one percent of subjects in the first year. This is the seventh multicenter randomized study of a collared T IUD with 380 mm2 of copper surface. In all seven, the one-year gross pregnancy rate has been 1.2 per 100 or lower.

Adult

Contraception with a vaginal ring releasing 3-keto desogestrel and ethinylestradiol.

Eleven healthy women used a contraceptive vaginal ring for three cycles. The rings released 15 micrograms of ethinylestradiol and 150 micrograms of 3-keto desogestrel per 24 hours. The rings were used for 21 days and then removed, rinsed in clean water and stored. After a 7-day ring-free period, the rings were reinserted. Totally, each ring was used for 3 cycles. The rings were well tolerated. The bleeding pattern was excellent. No woman showed any signs of ovulation when using the ring. Estradiol levels were suppressed, but showed an increase during the ring-free period as can be found during the pill-free period of an oral contraceptive regimen. When the ring was reinserted, the estradiol levels were rapidly suppressed again. SHBG capacity increased during use of the ring.

Adult

Clinical results with subcutaneous implants containing 3-keto desogestrel.

Eight healthy women using one Silastic implant of 30 mm length filled with 3-keto desogestrel, the active metabolite of desogestrel, were studied for 36-664 days. The release rate of 3-keto desogestrel was quite constant and around 30 micrograms/day. No ovulations occurred. One woman was amenorrheic, while the others had different bleeding patterns, often with periods of spotting. No other side effects were recorded. The mean plasma levels of 3-keto desogestrel were 0.9 nmol/l after 1 month and 0.5 nmol/l after 12 months. 3-Keto desogestrel was less effective than testosterone, estradiol or levonorgestrel in displacing 3H-dihydrotestosterone from SHBG. It is concluded that contraception with 3-keto desogestrel delivered through an implant is a promising method for further investigation. With a single implant we found a duration of at least one year.

Binding, Competitive

Ovarian function during use of a levonorgestrel-releasing IUD.

Ovarian function was studied for two complete menstrual cycles in 9 regularly menstruating women and for 8 weeks in three amenorrhoeic women who had used levonorgestrel-releasing IUDs (LNG-IUD) for more than four years. Nine patients using copper IUDs (Nova-T) were studied for two complete menstrual cycles as controls. According to progesterone levels, 15/17 cycles in women using LNG-IUDs were ovulatory, whereas only 8/17 cycles showed normal follicular growth and rupture as judged by ultrasound. In ovulatory cycles, the peak progesterone levels were lower than in the controls. The preovulatory estradiol and LH peak levels were also lower than in control subjects. SHBG levels were lower in LNG-IUD users than in copper IUD users. It is concluded that, although the dose of levonorgestrel released from the IUD is very low, it probably exerts an effect on the gonadotrophin secretion, which disturbs follicular development in many of the women studied, which in addition to the local effect on the endometrium, contributes to its high contraceptive efficacy.

Adult

Contraception with NORPLANT implants and NORPLANT-2 implants (two covered rods). Results from a comparative clinical study in Sweden.

Two-hundred-and-forty healthy women, ages 18 to 40 years, were randomized in a ratio of 2:5 to use NORPLANT implants or NORPLANT-2 implants. Through three years of use, no pregnancies were recorded among women using NORPLANT implants and two pregnancies were noted among women using NORPLANT-2 implants resulting in a cumulative net pregnancy rate of 1.3 +/- 0.9 per 100 acceptors (mean +/- SE) by the end of year three. This difference was not statistically significant. During the fourth year, no pregnancies were observed in the NORPLANT group, but 4 pregnancies occurred in the NORPLANT-2 group. The most common reason for terminating the study was bleeding disturbances. During the first year there were significantly more terminations due to bleeding problems in the NORPLANT group than in the NORPLANT-2 group. However, during the second year of use the proportion of women discontinuing for bleeding problems dropped considerably among NORPLANT users and during the third year very few women in either group discontinued because of bleeding problems. The continuation rates after one year were for NORPLANT users 59.4% and for NORPLANT-2 users 77.2%. Corresponding figures after three years of use were 46.1% and 51.7%, respectively. The second most common reason for discontinuation was depression and other mood changes. In both groups we noted a slight increase in weight during the study and a slight decrease in blood pressure and hemoglobin levels with time. In conclusion, both NORPLANT and NORPLANT-2 implants are very effective methods for contraception. The efficacy of NORPLANT-2 implants, however, was not acceptable during the fourth year of use in this study. The latter system could, however, become a suitable three-year contraceptive method, possibly with less bleeding disturbances than NORPLANT in the first year.

Adolescent

Plasma levels of levonorgestrel and free levonorgestrel index in women using NORPLANT implants or two covered rods (NORPLANT-2).

Plasma levels of levonorgestrel, sex hormone binding globulin (SHBG) and estradiol were studied during four years in 283 healthy women using either NORPLANT implants or two covered rods (NORPLANT-2). The women were randomized to use either type of implant. Both implant systems have previously been shown to have similar release rates of levonorgestrel. In both groups plasma levels of levonorgestrel decreased throughout the study, and there were no statistically significant differences between the two groups in mean plasma levels of levonorgestrel. During the study 8 women became pregnant. All pregnancies but one occurred after 35 months of implant use and only in women using the covered rods. No significant differences were seen between the women who became pregnant and the rest of the group using two covered rods with respect to plasma levels of levonorgestrel. SHBG capacity tended to be somewhat higher in women using the two covered rods. As levonorgestrel is to a great extent bound to SHBG, and in that form not biologically active, a "free levonorgestrel index" was calculated as a ratio between levonorgestrel and SHBG. This index was significantly lower in users of two covered rods than in users of NORPLANT implants at 1, 12 and 48 months of use. Women who became pregnant had significantly lower "free levonorgestrel index" than had the rest of the group. It is postulated that the difference in "free levonorgestrel index" between users of the two implant systems reflect differences in release rate, the covered rods having a lower release rate of levonorgestrel than NORPLANT throughout the observation period. It is concluded that "free levonorgestrel index" is a better parameter than levonorgestrel plasma levels to describe implant function, and to discriminate women who are at risk of pregnancy.

Adult

Plasma levels of cortisol and corticosteroid binding globulin during use of Norplant-2 implants.

Plasma levels of cortisol and corticosteroid binding globulin (CBG) were studied during one year in eleven healthy women using NORPLANT-2 implants. NORPLANT-2 implants release approximately 50 ug of levonorgestrel/day. A significant diurnal variation of cortisol levels was found during the study. No significant change in cortisol levels compared to pretreatment levels was found. Levels of CBG showed no diurnal variation, but decreased significantly during use of the implants. This was most probably due to a direct effect of the levonorgestrel, as the levels of estradiol did not change compared to pretreatment levels, except for the values after 6 months that were significantly lower than pretreatment levels. A "free cortisol index" calculated as: level of cortisol/level of CBG, was unchanged during treatment compared to pretreatment levels. It is concluded that although CBG levels were reduced, no significant change in cortisol patterns was seen during use of the implants.

Adult

General and local [corrected] aetiologic factors in canine osteochondrosis.

Observations based on necropsy findings in 89 dogs with osteochondrosis are presented. Osteochondrosis was most common in the elbow joint, followed by the shoulder joint, the stifle and the hock joint. There were 59 male and 30 female dogs, and the mean age was 2 years. The high frequency of bilateral lesions and the occurrence of osteochondrosis in more than one pair of joints in 25 of the 89 dogs indicates that general factors are important. The various manifestations of osteochondrosis and their frequency are reported. Various local factors believed to be important for the occurrence of the lesions within the joints are also considered.

Animals

Contraception with subdermal implants releasing levonorgestrel. A clinical and pharmacological study.

Two methods for release of levonorgestrel from polydimethylsiloxane (Silastic) subdermal implants for contraception were studied. The first system consists of six 3 cm long Silastic capsules filled with levonorgestrel (NORPLANT). The second one consists of two 4 cm long rods made from Silastic and levonorgestrel, covered with thin Silastic tubing (NORPLANT-2). The contraceptive efficacy of NORPLANT implants was excellent during the four years of observation. That of NORPLANT-2 implants was very good during the first three years, but not acceptable during the fourth year. Both types of implants were well tolerated and the most common side effect experienced was bleeding disturbances such as irregular bleeding and periods of prolonged bleeding. The frequency of such disturbances decreased considerably after the first year of use. The plasma levels of levonorgestrel decreased throughout the study, and did not show any statistically significant difference between users of the two types of implants. The plasma levels of levonorgestrel in women who became pregnant did not differ from those in women who did not become pregnant. Levonorgestrel binds to sex hormone binding globulin (SHBG) with high affinity, and in the bound form is not biologically active. A "free levonorgestrel index" (FLI), i.e. the ratio of plasma level of levonorgestrel to SHBG capacity, was calculated. Women with NORPLANT had higher FLI than those with NORPLANT-2 and women who became pregnant had lower FLI than those who did not. It is suggested that NORPLANT-2 implants have a lower rate of release of levonorgestrel than NORPLANT implants. SHBG capacity, thyroid binding proteins and corticosteroid binding globulin decreased during treatment. Plasma total testosterone decreased during treatment, whereas free testosterone was unaltered. Thyroid function and plasma cortisol levels did not change during treatment. Treatment with phenytoin decreased the contraceptive effect. In a group of regularly menstruating women who had used NORPLANT-2 implants for more than three years, 40% showed anovulation, 20% luteinization of unruptured follicles and 40% apparent ovulation.

Adolescent