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Biomedical subjects

S E Moss

Publications and source records attributed to S E Moss.

At least 109 records · Page 6Linked to original sources
PubMed1994

The influence of combined kidney-pancreas transplantation on the progression of diabetic retinopathy. A case series.

PURPOSE: To evaluate the impact of combined kidney and pancreas transplantation on the progression of advanced diabetic retinopathy. METHODS: The changes in diabetic retinopathy severity in patients with insulin-dependent diabetes mellitus who had kidney-pancreas transplantation (n = 51) and in those who had kidney transplantation only (n = 21) were compared. Patients were invited to baseline and 1 year follow-up examinations. Fundus photographs were graded in a masked fashion using standardized protocols. RESULTS: The mean age and duration of diabetes were similar for both groups. After combined transplantation, none of the patients used insulin, and their mean glycosylated hemoglobin was significantly lower (6.4% versus 10.6%) than those who underwent only kidney transplantation. There was a nonsignificant difference in overall progression of retinopathy for combined transplantation compared with kidney transplantation only (risk ratio = 0.73; 95% confidence interval, 0.31, 1.71). CONCLUSION: The authors found no evidence that the normalization of glycemia associated with a combined kidney-pancreas transplantation in patients with advanced proliferative diabetic retinopathy (mostly treated with photocoagulation) accelerated retinopathy progression. These data suggest that the normalization of glycemia associated with a combined kidney-pancreas transplantation does not have beneficial influence on the progression of advanced diabetic retinopathy.

Adult↗
PubMed1994

Ten-year incidence of visual loss in a diabetic population.

PURPOSE: The purposes of this study are to estimate the 10-year incidence of blindness and visual loss in a diabetic population, examine risk factors for visual loss, and examine temporal trends in rates of blindness and visual loss. METHODS: The design is a population-based cohort study, which includes 891 younger-onset persons with diabetes, 485 persons with older-onset diabetes who are taking insulin, and 502 persons with older-onset diabetes who are not taking insulin and who participated in baseline, 4-year, and 10-year examinations. The main outcome measures are incidence of blindness (visual acuity, 20/200 or worse), doubling of the visual angle, and visual impairment (visual acuity, 20/40 or worse). RESULTS: The 10-year incidences of blindness were 1.8%, 4.0%, and 4.8% in persons in the younger-onset, older-onset taking insulin, and older-onset not taking insulin groups, respectively. Respective 10-year rates of visual impairment were 9.4%, 37.2%, and 23.9%. Rates for doubling of the visual angle were similar. Doubling of the visual angle was more common in women with older-onset diabetes and in persons with younger-onset and older-onset diabetes who were taking insulin and who had higher glycosylated hemoglobin or proteinuria levels. The presence of macular edema or more severe retinopathy was associated with more visual loss in all three groups. Smoking was associated with visual loss in persons with younger-onset diabetes, and systolic blood pressure was associated in persons with older-onset diabetes who were taking insulin. Rates of blindness and doubling of the visual angle decreased in persons with younger-onset diabetes in the second part of the follow-up. In the older-onset groups, only the rate of blindness declined. These decreases could not be explained entirely by treatment or mortality. CONCLUSIONS: Several modifiable risk factors are associated with loss of vision. Although visual loss is still common in this diabetic population, the incidence of blindness may be decreasing.

Adolescent↗
PubMed1994

Ocular factors in the incidence and progression of diabetic retinopathy.

PURPOSE: To investigate the association of intraocular pressure (IOP), ocular perfusion pressure, and myopia with the incidence and progression of diabetic retinopathy. METHODS: The design is a cohort study of a population-based sample (n = 1210) of persons with younger-onset diabetes (diagnosis was made before 30 years of age, and subjects were taking insulin) and a random sample (n = 1780) of persons with older-onset diabetes (diagnosis made after 30 years of age). Baseline and 4-year follow-up examinations were completed by 891 younger-onset and 987 older-onset persons. Retinopathy was graded from stereoscopic fundus photographs. Endpoints were incidence of retinopathy, progression of retinopathy, and progression to proliferative diabetic retinopathy (PDR). Ocular perfusion pressure was calculated from IOP and blood pressure. Myopia was a refractive error of -2 diopters or less. RESULTS: In univariate analyses, ocular perfusion pressure was associated with incidence of retinopathy (P < 0.005), progression of retinopathy (P = 0.07), and progression to PDR (P < 0.001) in the younger-onset group but not in older-onset subjects taking or not taking insulin. Intraocular pressure and myopia were not associated with any endpoint in any group. Using logistic regression to control for covariates, ocular perfusion pressure was significantly associated only with incidence of retinopathy in younger-onset persons. The odds ratio for a 10-mmHg increase in ocular perfusion pressure was 2.13 (95% confidence interval, 1.30-3.50). Also, myopia was protective for progression to PDR in younger-onset persons with an odds ratio of 0.40 (95% confidence interval, 0.18-0.86). CONCLUSIONS: These results suggest that pressure phenomena may be related to the development of retinopathy in younger-onset persons. This would have implications for treatments affecting both IOP and blood pressure.

Adult↗
PubMed1994

The relation of socioeconomic factors to the incidence of proliferative diabetic retinopathy and loss of vision.

PURPOSE: To investigate the relations between socioeconomic factors and the incidence of proliferative retinopathy and loss of vision. METHODS: A population-based sample of younger-onset persons with diabetes 25 years of age or older (n = 334) and older-onset persons with diabetes (n = 906) was followed over a 4-year period. Education, occupational status, employment status, and marital status were measured at baseline and follow-up. Main outcome measures were incidence of proliferative retinopathy and loss of vision measured using standard protocols. RESULTS: Proliferative retinopathy was more likely to develop in younger-onset women with less education than in those with more education; no relation was found in the older-onset group. Education was associated inversely with incidence of loss of vision in younger-onset women and older-onset men. CONCLUSION: These data suggest that education is associated with the development of loss of vision, independent of other risk factors. The associations vary between men and women and between younger-onset and older-onset people with diabetes. Further understanding of these relationships may lead to interventions to prevent loss of vision in people with diabetes.

Adult↗
PubMed1994

Endocytosis occurs independently of annexin VI in human A431 cells.

Annexin VI is one of a family of calcium-dependent phospholipid-binding proteins. Although the function of this protein is not known, various physiological roles have been proposed, including a role in the budding of clathrin-coated pits (Lin et al., 1992. Cell. 70:283-291.). In this study we have investigated a possible endocytotic role for annexin VI in intact cells, using the human squamous carcinoma cell line A431, and report that these cells do not express endogenous annexin VI, as judged by Western and Northern blotting and PCR/Southern blotting. To examine whether endocytosis might in some way be either facilitated or inhibited by the presence of annexin VI, a series of A431 clones were isolated in which annexin VI expression was achieved by stable transfection. These cells expressed annexin VI at similar levels to other human cell types. Using assays for endocytosis and recycling of the transferrin receptor, we report that each of these cellular processes occurs with identical kinetics in both transfected and wild-type A431 cells. In addition, purified annexin VI failed to support the scission of coated pits in permeabilized A431 cells. We conclude that annexin VI is not an essential component of the endocytic pathway, and that in A431 cells, annexin VI fails to exert any influence on internalization and recycling of the transferrin receptor.

Annexin A6↗
PubMed1994

Self-rated health and mortality in people with diabetes.

OBJECTIVES: This study examined whether self-rated health is an independent and significant predictor of mortality in people with diabetes, using data collected in the Wisconsin Epidemiologic Study of Diabetic Retinopathy. METHODS: Participants were asked to rate their health in comparison with others their age. A proportional hazards model was used to regress survival time on self-rated health and a number of covariates measuring physical health. RESULTS: People with younger onset diabetes (n = 891) who rated their health relative to their peers as "worse" or "don't know" were no more likely to die than those rating their health as "the same" or "better" when physical health status was controlled. In contrast, those with older onset diabetes (n = 987) who rated their health as "worse" or "don't know" were almost twice as likely to die as those rating their health as "the same" or "better" when physical health status was controlled. CONCLUSIONS: Self-rated health is a significant predictor of mortality in people with older onset diabetes but not in those with younger onset diabetes when physical health status is controlled.

Adult↗
PubMed1994

Refractive status in the Beaver Dam Eye Study.

PURPOSE: To describe the prevalence of refractive errors in a population of adult Americans. METHODS: From 1988 to 1990, 4926 adults who were 43 to 84 years of age and living in Beaver Dam, Wisconsin at the time of the 1987-1988 census were examined. Refractions were performed according to a modification of the Early Treatment Diabetic Retinopathy Study protocol. Included in this study were 4533 people who had not undergone cataract surgery and who had a best corrected visual acuity better than 20/40 in at least one eye. Myopia was defined as a refractive error less than -0.50 diopters; hyperopia was defined as a refractive error greater than +0.50 diopters. RESULTS: Hyperopia was more frequent than myopia in the study group (age-adjusted of 49.0% and 26.2% in right eyes, respectively, P = 0.0001). The prevalence of hyperopia in the right eye increased with increasing age from 22.1% in those 43 to 54 years of age to 68.5% in those 75 years of age or older. The prevalence of myopia in the right eye decreased from 43.0% in those 43 to 54 years of age to 14.4% in those 75 years of age or older. There was significant relationship between education level and refractive error (age adjusted r = -0.32, P = 0.0001). Neither household income nor occupation was associated with refractive error in our data. CONCLUSION: These cross-sectional data indicate age-related differences in refractive status in an adult population and suggest that education is associated with myopia independent of age.

Adult↗
PubMed1993

Modulation of protein tyrosine phosphorylation during G1/S transition in activated human T-lymphoblasts.

We report that in activated human T-lymphoblasts synchronized early in the G1-phase of the cell cycle, addition of interleukin 2 (IL-2) stimulates transition into S-phase in conjunction with specific and reproducible protein tyrosine phosphorylation events. Prominent among these was de novo phosphorylation of the p56lck tyrosine kinase, which appeared as a single polypeptide on SDS-polyacrylamide gel electrophoresis prior to the addition of IL-2, but as a doublet of approximately 56 and 59 kDa 3 h after IL-2 addition. Although the lck polypeptide doublet persisted into S-phase, the 59-kDa form was virtually undetectable in T-lymphoblasts in log phase growth. In T-lymphoblasts metabolically labeled with 32Pi, antiphosphotyrosine antisera identified a major 56-kDa tyrosine-phosphorylated protein, whereas antisera to lck showed that phosphorylation of lck occurred on both the 56- and 59-kDa forms. Phosphoamino acid analyses identified phosphoserine as the major phosphoamino acid on both of the lck polypeptides, although small amounts of phosphotyrosine were also detected on the 56-kDa form. Immune complex kinase assays revealed that only the lower band of the lck doublet exhibited autocatalytic activity. Furthermore, the 59-kDa form neither displayed autocatalytic activity, nor was it a substrate for phosphorylation by the 56-kDa form. We conclude that entry into S-phase in activated human T-lymphoblasts is associated with tyrosine phosphorylation of a limited number of proteins and suggest that p56lck activity is regulated during G1/S.

Antigen-Antibody Complex↗
PubMed1993

Is there an ethnic difference in the effect of risk factors for diabetic retinopathy?

Mexican Americans have an increased prevalence and incidence of non-insulin-dependent diabetes mellitus (NIDDM). In addition, Mexican American diabetic subjects have an increased prevalence of retinopathy relative to Caucasian diabetic subjects. In Mexican American diabetic subjects, established risk factors may have a stronger effect on diabetic retinopathy, compared to Caucasian diabetic subjects. In this report, we compare the effect of established risk factors (age at examination, gender, age at diagnosis of diabetes, duration of diabetes, glycemia, type of therapy, systolic and diastolic blood pressure, and hypertension prevalence) between Caucasian diabetic subjects (n = 478), a low-risk population for NIDDM (Wisconsin Epidemiologic Study of Diabetic Retinopathy), and Mexican American diabetic subjects (n = 231), a high-risk population for NIDDM (San Antonio Heart Study). Retinopathy was classified into two categories (any or none) as assessed by seven standard stereoscopic retinal photographs read at the University of Wisconsin Reading Center. Mexican American diabetic subjects in Texas had an increased prevalence of any retinopathy (odds ratio = 1.71, 95% confidence interval: 1.25, 2.34), compared to Caucasian diabetic subjects in Wisconsin. Longer duration of diabetes, more severe glycemia, earlier age at diagnosis, and insulin therapy were associated with diabetic retinopathy in both Mexican Americans and Caucasians. Socioeconomic status was not associated with prevalence of retinopathy. Moreover, the effect of risk factors for retinopathy was similar in both ethnic groups.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1993

Sex hormones and the incidence of severe retinopathy in male subjects with type I diabetes.

BACKGROUND: Diabetic retinopathy rarely occurs before puberty, suggesting that changes in sex hormones may influence the development of this condition. METHODS: The authors measured total testosterone, free testosterone, sex hormone-binding globulin, estradiol, and dehydroepiandrosterone-sulfate levels in 44 men with type I diabetes participating in the 1984 to 1986 re-examination phase of the Wisconsin Epidemiologic Study of Diabetic Retinopathy, a population-based study of diabetic complications. The mean age was 32.7 years, and the mean duration of diabetes was 15.4 years. Subjects who progressed to proliferative or preproliferative retinopathy 6 years later (cases, n = 22) were compared with subjects who had little or no progression (controls, n = 22). Both groups were matched on initial level of retinopathy. Seven stereoscopic retinal photographs of each eye were obtained at both examinations, and the photographs were read by the University of Wisconsin Reading Center. RESULTS: Sex hormone-binding globulin concentrations (nmol/l) were significantly lower (reflecting increased androgenicity) in cases than in controls (22.4 +/- 11.4 versus 32.5 +/- 19.8; P = 0.04). No other statistically significant differences in hormone levels were observed. CONCLUSION: Neither serum testosterone nor other sex hormones were related to the incidence of severe retinopathy. The relationship to low-serum sex hormone-binding globulin suggests that increased androgenicity may be associated with the progression of retinopathy in male subjects with type I diabetes.

Adult↗
PubMed1993

Is gross proteinuria a risk factor for the incidence of proliferative diabetic retinopathy?

PURPOSE: To examine the relationship between gross proteinuria, measured at the initial examination, and the 4-year incidence of proliferative diabetic retinopathy (PDR) in a population-based study in Wisconsin. METHODS: Gross proteinuria was measured by reagent strip. Diabetic retinopathy was determined from stereoscopic fundus photographs in a masked fashion using the modified Airlie House classification scheme. RESULTS: In the younger-onset group taking insulin (n = 693), the relative risk of proliferative retinopathy developing in those with gross proteinuria at baseline was 2.32 (95% confidence interval [CI]: 1.40,3.83) compared with those without gross proteinuria. For the older-onset group taking insulin, the relative risk was 2.02 (95% CI: 0.91,4.44), and for those not taking insulin it was 1.13 (95% CI: 0.15,8.50). After controlling for other risk variables, the relationship was of borderline statistical significance (P = 0.052) in the younger-onset group with no or early nonproliferative retinopathy at baseline. CONCLUSION: These data suggest that gross proteinuria is a risk indicator for proliferative retinopathy in younger-onset patients with diabetes. These patients might benefit from having regular ophthalmologic evaluation.

Adult↗
PubMed1993

The association of microalbuminuria with diabetic retinopathy. The Wisconsin Epidemiologic Study of Diabetic Retinopathy.

PURPOSE: To investigate the relationship between microalbuminuria and the presence and severity of diabetic retinopathy in a large population-based cohort of individuals with diabetes. METHODS: Microalbuminuria was measured by an agglutination inhibition assay in random urine samples obtained from participants in the Wisconsin Epidemiologic Study of Diabetic Retinopathy (n = 1139) who did not have hematuria, gross proteinuria, or a history of renal disease. Retinopathy was determined from stereoscopic color fundus photographs graded according to a modification of the Airlie House Classification System. RESULTS: Younger-onset (diagnosed with diabetes before 30 years of age) and older-onset (diagnosed with diabetes when 30 years of age or older) individuals with microalbuminuria were more likely to have retinopathy than those without microalbuminuria. Younger-onset individuals who had microalbuminuria at the time of examination were more likely to have proliferative retinopathy than younger-onset subjects with normoalbuminuria. These relationships remained after controlling for glycemia, hypertension, duration of diabetes, and other potential confounders. CONCLUSIONS: Microalbuminuria is associated cross-sectionally with the presence of retinopathy in persons with diabetes and with the presence of proliferative disease in younger-onset individuals. These data suggest that microalbuminuria may be a marker for the risk of proliferative retinopathy developing. If longitudinal studies confirm these findings, individuals with insulin-dependent diabetes mellitus (IDDM) who have microalbuminuria may benefit from ophthalmologic follow-up.

Adult↗
PubMed1993

Incidence of gross proteinuria in older-onset diabetes. A population-based perspective.

A few population-based studies describe the incidence of gross proteinuria in people with diabetes. We performed a population-based study in southern Wisconsin of diabetic individuals diagnosed at > or = 30 yr of age either taking insulin (n = 398) or not taking insulin (n = 441). The presence of gross proteinuria (> or = 0.3 g/L) was determined by means of a reagent strip. The incidence of proteinuria in the 4-yr interval was 17.3% (95% CI 13.6-21.0) in those taking insulin and 10.7% (95% CI 7.8-13.6) in those not taking insulin. The relative risk of developing proteinuria for those in the highest level of total pack-yr smoked compared with those who had never smoked was 2.0 (95% CI 1.2-3.3) for those taking insulin and 2.5 (95% CI 1.3-4.5) for those not taking insulin. After controlling for other risk variables, the incidence of gross proteinuria was also associated with higher GHb. These data suggest that smoking and glycemic control, both potentially modifiable factors, are significant risk factors for the development of gross proteinuria.

Adult↗
PubMed1993

Prevalence of microalbuminuria in older-onset diabetes.

OBJECTIVE: To examine the prevalence of microalbuminuria and the relationships of microalbuminuria to blood pressure and other risk factors. RESEARCH DESIGN AND METHODS: Individuals diagnosed with diabetes at > or = 30 yr of age either taking insulin (n = 435) or not taking insulin (n = 363), who were participants in the population-based Wisconsin Epidemiologic Study of Diabetic Retinopathy, were examined during 1984-1986. Random urine samples were collected and an agglutination inhibition test was used to determine the presence of microalbuminuria, which is defined as > or = 0.03 g/L but < 0.3 g/L. RESULTS: The frequency of microalbuminuria was 29.2% in those individuals taking insulin and 22.0% in those not taking insulin. Microalbuminuria was significantly associated with the male sex, older age, higher systolic blood pressure, higher GHb, use of insulin, higher recent alcohol consumption, and a history of cardiovascular disease. CONCLUSIONS: These findings suggest a relationship between controllable risk factors, blood pressure and GHb, and microalbuminuria in older-onset diabetic individuals.

Adult↗
PubMed1993

Blood pressure, hypertension and retinopathy in a population.

This study provides precise estimates of the prevalence of retinal lesions in nondiabetic persons with and without hypertension. The findings suggest that retinopathy (6% in normotensives and 11% in people with hypertension), and retinal arteriolar narrowing (11% in normotensives and 19% in people with hypertension) are common. Further longitudinal study is necessary to evaluate the public health significance of these findings.

Adult↗
PubMed1992

A growth-dependent post-translational modification of annexin VI.

Annexin VI (p68, 67-kDa calelectrin) is a member of a family of Ca2+/phospholipid-binding proteins, that includes p35 (annexin I) and p36 (annexin II), the major cellular substrates for phosphorylation by the epidermal growth factor receptor and pp60v-src tyrosine kinase activities, respectively. We report here that like annexins I and II, annexin VI is phosphorylated in vivo, but that in contrast, annexin VI phosphorylation is associated with cell growth. In both Swiss 3T3 fibroblasts and human T-lymphoblasts the pattern of phosphorylation followed an almost identical profile. In particular, annexin VI was not phosphorylated in quiescent cells, but was phosphorylated on serine and to a lesser extent threonine, several hours following cell stimulation. Furthermore, annexin VI also incorporated phosphate in a growth-dependent manner, in a form other than a phosphoamino-acid. The phosphate was visualised following acid hydrolysis of immunoprecipitated annexin VI, as part of a complex having high mobility on 2-D thin-layer electrophoresis. The identity of this complex is not known. The results suggest that a post-translational modification other than direct protein phosphorylation may influence the activity of annexin VI and provide evidence linking cell growth with regulation of annexin VI function.

3T3 Cells↗