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Biomedical subjects

S E Miller

Publications and source records attributed to S E Miller.

81 records · Page 5Linked to original sources
PubMedDate not supplied

Primary actinomycosis of an extremity: a case report and review.

Primary infection of an extremity is an uncommon feature of actinomycosis and can readily be confused with actinomycetoma caused by aerobic actinomycetes such as Nocardia and Streptomyces. A case of primary actinomycosis of the leg is reported, and 35 cases published in the English-language literature are reviewed. There were 14 cases of upper extremity infections and 22 cases of lower extremity infections. Antecedent trauma had occurred in 21 cases. Lesions began in the skin, subcutaneous tissue, muscle, bone, and joint space. Spread to contiguous structures was characteristic, and most lesions eventually involved all of the aforementioned structures. The lesions were typical of actinomycosis with sinus formation and granules in the pus. Cultures were positive in 17 cases. Surgical therapy was necessary in most instances; penicillin remains the drug of choice. Pending results of culturing, a lesion with granules of branching gram-positive bacteria would best be treated empirically with penicillin and a sulfonamide. Prognosis is excellent.

Actinomyces↗
PubMedDate not supplied

Using a process action team to improve patient education.

To develop an effective, coordinated, and integrated process for patient education in primary care, the Department of Veterans Affairs, Western New York Healthcare System, launched a process action team. The interdisciplinary team utilized a variety of methods, including flowcharts, cause-and-effect diagrams, and variance matrices. The team developed, implemented, and analyzed a data collection plan involving chart reviews, staff interviews, and patient-staff surveys. An improvement plan was initiated in collaboration with staff. The results highlight the importance of using a data collection plan to understand the causes of problems as well as the need for staff involvement.

Education, Continuing↗
PubMedDate not supplied

Identification of focal viral infections by confocal microscopy for subsequent ultrastructural analysis.

A correlative microscopy method for the ultrastructural analysis of focal viral tissue infections is presented. Using a confocal scanning laser microscope, foci of infection are identified in tissue sections prior to embedment; a variety of techniques can be employed for viral detection, including staining with standard histochemical reagents and fluorescently labeled antibodies. Areas of infection identified using confocal microscopy are excised from the tissue sections, embedded, and examined by transmission electron microscopy. Applications of this technique in both diagnostic and basic research settings are described.

Animals↗
PubMedDate not supplied

Concerted use of immunologic and ultrastructural analyses in diagnostic medicine: immunoelectron microscopy and correlative microscopy.

Electron microscopy (EM) is a valuable tool in diagnostic medicine, and in some cases, can be enhanced by immunological methods. A major medical application of EM, diagnostic virology, can frequently be augmented by employment of immunological reagents. Three immunoelectron microscopy (IEM) methods, aggregation, coating, and gold labeling, provide means for serotyping viruses; aggregation by antibody can also be used to concentrate viruses in dilute suspension or to serotype them. As a research tool, IEM can be useful in studying the relationship of various pathogen proteins to the infected cells or tissues. Delineating the subcellular location of viral components can yield information about how virions are constructed, and hence, suggest methods and compounds for inhibiting that process. Conversely, labeling virus-infected cells with antibodies against various cell receptors and proteins can yield information about the association of the proteins with budding virions. Another research example is the identification by immunological staining of virus-infected cells for subsequent ultrastructural identification of the specific cell type involved. Electron microscopy and immunolabeling methods are also useful in the diagnosis of immune complex disorders, including various forms of postinfectious immune complex glomerulonephritis. Precise analysis of immune complex deposits can be accomplished by using EM to pinpoint their location and immunohistology to probe their composition. Finally, a variety of optical microscopic techniques, including some involving immunofluorescent labeling, can be used to identify areas of interest in inhomogeneous tissues for further study by EM.

Child↗
PubMedDate not supplied

Nurses' attitudes toward nursing process as measured by the Dayton Attitude Scale.

This study measured the knowledge of and attitudes toward the nursing process held by nurses who provide direct patient care. The Dayton Attitude Scale Toward Care Planning (DASC) was returned by 1,096 nurses practicing in nine hospitals. Overall, participants were knowledgeable of the nursing process and held a relatively positive attitude toward the nursing process and nursing diagnosis. Higher nursing degrees were associated with more positive attitudes. The most common barrier to the use of the nursing process was insufficient time. The study found that less than 30% of the subjects liked the way care planning was done. These findings suggest that improving care planning systems would further promote consistency in the process.

Educational Status↗
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Isolation and identification of a turkey respiratory adenovirus.

A virus with physical and biological characteristics of an adenovirus was isolated from turkey poults with respiratory disease. The virus was ether-resistant and incorporated [3H] thymidine. Electron microscopy revealed virions of icosahedral configuration, approximately 78 nm in diameter, within the nuclei of infected cells. The virus produced cytopathology in turkey kidney cells, but did not produce observable disease when inoculated into commercial turkey poults or specific-pathogen-free embryonated chicken eggs. Virus-neutralization tests indicated widespread exposure to the virus in North Carolina turkey populations.

Adenoviridae↗
PubMedDate not supplied

Physicochemical characterization and pathogenicity studies of two turkey adenovirus isolants.

Two turkey adenoviruses were isolated from poults with respiratory disease, and their physicochemical properties were studied. The virus particles were unenveloped. contained DNA genome, replicated within the nuclei of infected cells, and were icosahedral in shape. Pathogenicity studies in poults indicated that North Carolina Kennedy isolant was a pathogen of relatively low virulence whereas the North Carolina Jackson isolant was nonpathogenic in commercial turkey poults.

Adenoviridae↗
PubMedDate not supplied

Isolation of an etiologic agent of acute respiratory disease (rhinotracheitis) of turkey poults.

A small gram-negative motile bacillus was isolated from laboratory poults affected by acute respiratory disease (rhinotracheitis) of turkeys. The bacterium was inoculated intranasally into susceptible day-old poults; the poults developed typical clinical signs of acute respiratory disease, and the bacterium was reisolated. This same bacterium was isolated from commercial poults with typical signs of acute respiratory disease but not from poults of similar age which were clinically normal. The bacterium has not been identified taxonomically. We conclude that it is a primary etiologic agent for acute respiratory disease of turkey poults.

Animals↗