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Biomedical subjects

S Diehl

Publications and source records attributed to S Diehl.

27 records · Page 2Linked to original sources
PubMed1988

Light microscopical morphometric classification of oncocytic and chromophobe pituitary adenomas.

Light microscopical morphometry was performed on 10 adenomas with oncocytic parts (26%-50% oncocytes, group I), on 9 oncocytic adenomas with 51%-75% oncocytes (group II), and on 12 oncocytic adenomas with 76%-100% oncocytes (group III). We measured the area and circumference of nuclei and areas of cells. Furthermore, shape factors for area and circumference of nuclei and the quotients of nuclei area/cell area were calculated. Data of about 40,000 single measurements were evaluated statistically. There is a clear positive correlation of cell areas compared to the proportion of oncocytes in the respective adenoma. A gradual, steady enlargement of cells can be observed, whereas enlargement of nuclei is seen to a much less extent. Consequently the quotients of area of nuclei/area of cells become smaller with increasing oncocytic transformation, evidence that enlargement of cells takes place in favour of cytoplasm. The three adenoma groups differ significantly from each other in their average cell sizes. Regarding nuclear sizes, the difference is mainly to be seen between group II and III. Comparison of adenomas containing oncocytes with large cell and small cell chromophobe adenomas also show significant differences in sizes of cells and nuclei. Large cell chromophobe adenomas show distinctly larger nuclei, which is looked upon as a display of their endocrine activity. On the other hand, oncocytic adenomas bear comparatively small nuclei in an extended cytoplasm which may well be related to their endocrine inactivity.

Adenoma, Chromophobe↗
PubMed1988

Immunohistochemical analyses of oncocytic and chromophobe pituitary adenomas.

Immunohistochemical analyses using antibodies against the major pituitary hormones, the alpha-subunit of glycoprotein hormones, chromogranin and synaptophysin were performed on 10 adenomas with oncocytic parts (26%-50% oncocytes, Group I), on 9 oncocytic adenomas with 51%-75% oncocytes (Group II), and on 12 oncocytic adenomas with 76%-100% oncocytes (Group III). Only 11 of the 31 investigated adenomas (35%) showed negative immunostaining for all major anterior pituitary hormones. FSH-content could be shown in 16 of 31 adenomas (52%), LH-content in 12 of 31 adenomas (39%), TSH-content in 3 of 31 adenomas (10%). Comparing all three groups of adenomas, there are no differences in the immunoreactivity to alpha-subunit (24 of 31 adenomas, 77%), chromogranin (26 of 31 adenomas, 84%), and of synaptophysin (13 of 31 adenomas, 42%). Considering the high percentage of cells of oncocytes showing alpha-subunit immunoreactivity we regard oncocytomas as originating very often from TSH-gonadotropin cell complexes of the anterior hypophysis. Alpha-subunit might become a reliable marker for oncocytomas. The finding of immunoreactivity to chromogranin in most cases confirms morphological studies that oncocytes contain some secretory granules. In most cases, the studied oncocytomas did not react to synaptophysin showing different results from other adenomas of the anterior hypophysis.

Adenoma↗
PubMedDate not supplied

Portal vein erosion and acute abdominal hemorrhage as a complication of acute pancreatitis.

The case of a 35-year-old male with portal vein erosion and acute life-threatening abdominal hemorrhage as a complication of acute pancreatitis is reported. Erosion of arterial walls is the most common complication of pancreatitis and pancreatic pseudocysts, and erosion of the portal vein leading to a fistula between the pancreas and the portal vein is very rare. Diagnosis was made by multislice spiral computed tomography, and nonsurgical treatment was elected, leading to complete recoverage.

Acute Disease↗
PubMedDate not supplied

Comparison of the detection of cervical human papillomavirus infection by filter DNA hybridization of cytologic specimens and by in situ DNA hybridization of tissues.

Cellular samples and subsequent cone biopsy samples from the same site in 18 patients were screened for infection with human papillomavirus (HPV) types 16 and 18 (HPV 16/18) by DNA hybridization. Filter hybridization of cells collected using cervical swabs was significantly less sensitive (with only 4 positive results) in detecting HPV 16/18 DNA sequences than was in situ hybridization of tissue sections (with 16 positive results). The in situ hybridization results correlated well with the cytologic and histologic findings of cervical intraepithelial neoplasia of grades II (mild dysplasia) and III (severe dysplasia and carcinoma in situ).

Autoradiography↗
PubMedDate not supplied

Gender reassignment in children: ethical conflicts in surrogate decision making.

Parents, as surrogate decision makers for their infants, are generally expected by health care providers to act in the child's "best interest." However, when the wishes of the parents are contrary to those of the medical profession, an ethical dilemma occurs. Whose decisions are best for the infant? Whose recommendations are in the child's best interest? This article explores an issue that may not involve a life and death decision, but one in which the parents clearly refuse expert medical recommendations for what the providers believe are in the best interest of the infant. Specifically, the case study explores the parents' refusal to proceed with gender reassignment to female for their genetic XY baby born with micropenis and nonpalpable testes. The issue calls into question the possible limits of surrogate decision making. The ethical principles of beneficence, respect for persons, nonmaleficence, and justice are addressed in relation to this particular case.

Adult↗