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Biomedical subjects

S Darougar

Publications and source records attributed to S Darougar.

At least 55 records · Page 3Linked to original sources
PubMed1984

Prevalence of acute conjunctivitis caused by chlamydia, adenovirus, and herpes simplex virus in an ophthalmic casualty department.

The causes of acute conjunctivitis in 140 consecutive patients attending an ophthalmic casualty department in London were investigated. Laboratory evidence of infection with Chlamydia trachomatis, adenovirus, or herpes simplex virus was found in 52 (37%) cases. In 70 cases (50%) no evidence of infection with these agents was found. In the remaining 18 cases (13%) laboratory results were inconclusive. The importance of these findings, the role of laboratory investigations in the differential diagnosis of acute conjunctivitis, and their cost-benefit are discussed.

Acute Disease↗
PubMed1983

Trachoma.

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Adolescent↗
PubMed1983

Perihepatitis.

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Chlamydia Infections↗
PubMed1983

Clinical and epidemiological features of adenovirus keratoconjunctivitis in London.

Ninety-eight patients were studied. Ninety were consecutive patients who were isolation-positive for adenovirus, and 8, who were associated with a hospital outbreak of adenovirus serotype 8 infection, developed characteristic features of infection but were isolation-negative. The ratio of males to females was 2 to 1, and most patients were aged 20 to 39. Adenovirus serotypes 3, 7, and 8 were isolated from 86% of patients, and serotypes 2, 4, 5, 11, 15, and 15/29 from the remaining 14%. Adenovirus serotype 7 was more commonly isolated from patients under the age of 19 and was Not isolated during winter. Sources of infection could be identified in 36% of patients and included contact with upper respiratory tract of ocular infections, a hospital outbreak, and a recent visit to a swimming pool. Associated systemic disease was detected in 47% of patients, most of whom had upper respiratory tract infection. The most severe and prolonged conjunctivitis was caused by serotypes 5 and 8. Most patients developed epithelial punctate keratitis. Subepithelial punctate keratitis, which was once-considered to be a characteristic feature of adenovirus serotype 8, developed in cases of serotype 3, 4, 5, 7, and 8 infection.

Adenoviridae Infections↗
PubMed1983

Unusual features in the conjunctiva and cornea of the normal guinea-pig: clinical and histological studies.

Clinical and histological studies of the outer eyes of newborn and adult female guinea-pigs were carried out together with histological studies of fetal eyes. Fine superficial corneal vessels, extending from the limbus, were seen in most newborn and adult eyes and were also present in histological preparations of fetal eyes. These vessels were therefore considered to be features of the normal guinea-pig eye. Masses of tissue composed of lymphoid cells, which were often organised into germinal centres, were found in the fornices of newborn and adult eyes. Smaller accumulations of lymphoid cells were found in fetal eyes, and this lymphoid tissue was therefore also considered to be a normal feature of the guinea-pig eye. In adult eyes mild but short-lived oedema or hyperaemia of the palpebral conjunctiva was quite common. There were no differences in the amounts of conjunctival cellular infiltration in these eyes c found in fetal eyes, and this lymphoid tissue was therefore also considered to be a normal feature of the guinea-pig eye. In adult eyes mild but short-lived oedema or hyperaemia of the palpebral conjunctiva was quite common. There were no differences in the amounts of conjunctival cellular infiltration in these eyes c found in fetal eyes, and this lymphoid tissue was therefore also considered to be a normal feature of the guinea-pig eye. In adult eyes mild but short-lived oedema or hyperaemia of the palpebral conjunctiva was quite common. There were no differences in the amounts of conjunctival cellular infiltration in these eyes compared with normal eyes, and these inflammatory changes were therefore not considered to be significant.

Animals↗
PubMed1983

Adenovirus serotypes isolated from ocular infections in London.

During the period 1973-8 700 adenoviruses were isolated from the eyes of patients presenting at Moorfields Eye Hospital. Of these, 678 were serotyped by a neutralisation test. Twenty-one different serotypes were identified. Serotype 3, 7, and 10 accounted for 68% of the isolates, 4, 8, 15 (15/29), and 19 for 25%, and the other 14 serotypes for 7%. Community outbreaks of ocular infections by adenovirus 3, 4, 7, 10, and 15 (15/29) were observed. Outbreaks with adenovirus 3, 7, and 10 appeared to continue for 2 years or more, whereas outbreaks with 4 and 15 (15/29) were restricted to one year or less. Hospital outbreaks by adenovirus 8 and 19 were also recorded. During the same period 18 of these 21 adenovirus serotypes were isolated from the nonocular sites (mainly respiratory tract) in 7804 cases. There was a close association in the distribution of adenovirus 1, 2, 3, 5, and 6 in the ocular and nonocular sites. No such association was observed for adenovirus serotypes 4, 8, 10, 15 (15/29), and 19.

Adenoviridae Infections↗
PubMed1983

Chlamydial genital infection in prostitutes in Iran.

The prevalence of chlamydial genital infection was studied in 177 prostitutes in Iran; 100 in Teheran and 77 in the port of Bandar Abbas. Chlamydia trachomatis was isolated in eight (6.9%) of 116 patients with valid cultures. Type-specific antibodies were found against C trachomatis serotypes D to K (genital serotypes) in 94.2% and against serotypes A to C (trachoma serotypes) in 2% of the prostitutes. Type-specific IgM at a titre of greater than or equal to 8, indicating current infection, was found in 29.2%, whereas type-specific IgG at a titre greater than or equal to 64, suggesting a current or recent infection, was present in 71.5%. The lower chlamydial isolation rate in these women may have been due to previous treatment with antichlamydial drugs and because of immune responses resulting from repeated reinfection with chlamydiae. The results indicate that in Iran prostitutes are commonly infected with C trachomatis and are probably a major reservoir of chlamydial genital infection.

Adolescent↗
PubMed1982

Biliary-type pain as a manifestation of genital tract infection: the Curtis-Fitz-Hugh syndrome.

Ten patients with biliary-type pain, in whom investigations of the biliary tract were negative, are reported. All the patients were sexually active premenopausal women and all had evidence of infection with chlamydia trachomatis. Five patients submitted to laparoscopy had fibrinous adhesions between the anterior surface of the liver and the parietal peritoneum (perihepatitis). All 10 patients were diagnosed as suffering from the Curtis-Fitz-Hugh syndrome caused by Chlamydia trachomatis. The clinical similarities between the Curtis-Fitz-Hugh syndrome (right upper quadrant abdominal pain, perihepatitis and genital tract infection) and acute biliary disease are emphasized and the diagnostic implications discussed.

Adolescent↗
PubMed1982

A rapid method for staining inclusions of Chlamydia psittaci and Chlamydia trachomatis.

A new staining method was developed for the detection of inclusions of Chlamydia psittaci and Chlamydia trachomatis inclusions in cell cultures. Using a combination of methyl green and neutral red stains and washing at pH 5.0, inclusions were stained red while cell cytoplasm was pale pink and cell nuclei were pale green. The method was significantly better than Giemsa staining and comparable to immunofluorescence for detecting C psittaci inclusions. Its sensitivity for detection C trachomatis inclusions by dark field microscopy was similar to that of Giemsa staining.

Animals↗
PubMed1982

Chlamydial genital infection in Ibadan, Nigeria. A seroepidemiological survey.

Sera from patients attending a sexually transmitted diseases (STD) clinic, a family planning clinic, and an antenatal clinic in Ibadan, Nigeria, as well as from male blood donors from the same area were tested for the presence of type specific antichlamydial antibodies using a modified micro-immunofluorescence test. Among men and women attending the STD clinic the exposure rates to Chlamydia trachomatis serotypes D to K (genital pathogens) were 18.7% and 26.7% respectively. Antibody titres suggesting active disease in these men and women were found in 11.8% and 22.7% respectively. The highest rate of exposure (35%) was among women attending the family planning clinic; of these women 25% had antibody suggesting active disease. Titres of IgG antibody in this study were similar to those found among men and women with chlamydial genital infections in the United Kingdom. Antibodies to serotypes D to K were also detected in 10.3% of women attending an antenatal clinic and in 9.9% of male blood donors. The prevalence of antibodies to C trachomatis serotypes A to C and lymphogranuloma venereum serotypes was low. These results suggest that the prevalence of chlamydial genital infections in Ibadan, both among STD patients and especially among those individuals not seeking treatment (family planning and antenatal clinic patients), is high. Since serious sequelae can follow chlamydial genital infections it is imperative to carry out further investigations in this area.

Antibodies, Bacterial↗
PubMed1982

Chlamydial genital infection in Addis Ababa, Ethiopia. A seroepidemiological survey.

A seroepidemiological survey was undertaken in Addis Ababa to assess the prevalence of chlamydial genital infections among patients attending a sexually transmitted diseases (STD) clinic and patients with no overt genital symptoms. In the STD clinic patients antibodies to Chlamydia trachomatis serotypes D to K (genital types) were detected in 68 of 210 (32.4%) men and in 72 of 159 (45.3%) women, a rate of exposure as high or higher than that found in Europe. Serological evidence of active chlamydial infection was present in 26.7% of men and 28.9% of women. Women were at risk of contracting STD, including chlamydial infections, at the age of 14 years or earlier. The titres of antichlamydial IgG were extremely high in some patients attending the STD clinic, with titres of between 1/512 and 1/8192 in 9.5% of men and 13.2% of women. This suggests that some patients had severe or disseminated chlamydial disease. The prevalence of exposure to chlamydial genital infections among 148 patients with no overt genital disease was 14.2%, which is significantly higher than that found in the United Kingdom. Among the total of 517 patients tested the prevalence of exposure to trachoma, lymphogranuloma venereum, and Chlamydia psittaci agents was very low.

Adolescent↗
PubMed1982

Chlamydial urethral infection in Teheran. A study of male patients attending an STD clinic.

The prevalence of chlamydial infection of the urethra was studied in 172 consecutive male patients attending a sexually transmitted disease clinic in Teheran. Chlamydia trachomatis was isolated in 8.8% of the patients with a valid culture result. Of the five isolates serotyped, two were serotype E and three were serotypes G, H, and K. Type-specific antibodies against C trachomatis serotypes D to K were found in 16% of patients, and IgM, indicating current infection, was detected in 12%. Type-specific antibodies against serotypes A to C (trachoma agent) were detected in 5.4%. The low chlamydial isolation rate may have been due to the inclusion of a large number of patients with a mild or trivial urethritis or a history of previous treatment with antichlamydial drugs. The results indicate that in Iran where trachoma is still endemic, chlamydial infection of the urethra does occur in the urban population and is caused by serotypes D to K.

Adolescent↗
PubMed1981

Effect of centrifugation on herpes simplex virus isolation.

The effects of high-speed centrifugation on the isolation of herpes simplex virus (HSV) were studied. Aliquots of laboratory or clinical specimens were inoculated into test tubes and flat-bottomed tubes containing HEp2 monolayers. Test tubes were incubated at 35 degrees C on roller drums (standard method), and flat-bottomed tubes were centrifuged at 15,000g at 35 degrees C for 1 hr, before being incubated at 35 degrees C without rolling (centrifuged method). Centrifugation of clinical and laboratory specimens of HSV type 1 and HSV type 2 produced significantly increased isolation rates compared with the standard method. When clinical and laboratory specimens were diluted, the centrifuged method was more sensitive at all dilutions. When 20 specimens were used for end-point titrations, the centrifuged method was 10 times more sensitive for 15 specimens and 100 times more sensitive for five specimens. There was no difference in the time taken for the appearance of cytopathic effect (CPE) between the standard and centrifuged methods.

Cells, Cultured↗
PubMed1981

[Chlamydial disease of the eye: a report on 27 cases. Clinical picture, differential diagnosis, treatment (author's transl)].

During the past five years a presumptive diagnosis of ocular chlamydial disease has been made in 90 patients consulting the outpatient department of Freiburg University Eye Clinic. The diagnosis was confirmed by different methods (giemsa stains, chlamydia cultures and serology, electron microscopy and histology) in 27 cases. The clinical spectrum included inclusion blennorrhea in newborns as well as inclusion conjunctivitis, TRIC keratoconjunctivitis and sporadic trachoma in adults. Presumably the incidence of the disease is much higher than indicated by our figures. We suspect a very high number of undiagnosed cases. These patients usually do not receive proper therapy (tetracyclin or erythromycin for at least three weeks) and their disease may run a protracted course with the risk of permanent eye damage.

Adolescent↗
PubMed1981

Transmission of chlamydiae by the housefly.

The ability of the housefly to carry viable Chlamydia trachomatis and to transmit a chlamydial ocular infection was studied under laboratory conditions. After feeding flies (Musca domestica) on suspensions of egg yolk sac infected with C. trachomatis serotypes A or B (responsible for hyperendemic trachoma) the agents were reisolated from flies' intestines for up to 6 hours and from their legs and/or proboscises for up to 2 hours. It was found that the viability of chlamydiae is dependent on the protective effect of yolk concentration in the original inoculum. Results of experiments with guinea-pig inclusion conjunctivitis as an animal model show that under laboratory conditions flies can readily transmit this chlamydial ocular infection from one animal to another. These results suggest that under field conditions flies can play an important role in the transmission of trachoma, particularly in areas with favourable conditions such as a large reservoir of infection among children with severe trachoma, copious eye discharge caused by trachoma and associated bacterial infections, a large fly population, and close proximity of children in large family groups.

Animals↗
PubMed1981

A comparison of the efficiency of human embryo intestine, Hep 2 cells, and human embryo kidney cells for the primary isolation of ophthalmic viruses.

A comparison has been made of the efficiency of 3 cell systems, human embryo kidney (HEK), Hep 2, and a continuous line of human embryo intestine (HEI), for the isolation of ophthalmic viruses. A total of 40 herpes simplex, 51 adenoviruses, and 2 vaccinia viruses were isolated from 323 specimens. HEK cells were found to be the optimal system, 85 out of 93 (91%) of the viruses being detected in these cells alone. However, HEK cells are difficult to obtain, and therefore the use of a combination of the continuous cell lines HEI and Hep 2 is recommended as an alternative. 89% of the viruses were detected by this combination. The use of either HEI or Hep 2 cells alone was unsatisfactory.

Adenoviruses, Human↗