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Biomedical subjects

S D Rao

Publications and source records attributed to S D Rao.

At least 19 recordsLinked to original sources
PubMed2001

Development of a novel immunoradiometric assay exclusively for biologically active whole parathyroid hormone 1-84: implications for improvement of accurate assessment of parathyroid function.

We developed a novel immunoradiometric assay (IRMA; whole parathyroid hormone [PTH] IRMA) for PTH, which specifically measures biologically active whole PTH(1-84). The assay is based on a solid phase coated with anti-PTH(39-84) antibody, a tracer of 125I-labeled antibody with a unique specificity to the first N-terminal amino acid of PTH(1-84), and calibrators of diluted synthetic PTH(1-84). In contrast to the Nichols intact PTH IRMA, this new assay does not detect PTH(7-84) fragments and only detects one immunoreactive peak in chromatographically fractionated patient samples. The assay was shown to have an analytical sensitivity of 1.0 pg/ml with a linear measurement range up to 2,300 pg/ml. With this assay, we further identified that the previously described non-(1-84)PTH fragments are aminoterminally truncated with similar hydrophobicity as PTH(7-84), and these PTH fragments are present not only in patients with secondary hyperparathyroidism (2 degrees -HPT) of uremia, but also in patients with primary hyperparathyroidism (1 degrees -HPT) and normal persons. The plasma normal range of the whole PTH(1-84) was 7-36 pg/ml (mean +/- SD: 22.7 +/- 7.2 pg/ml, n = 135), whereas over 93.9% (155/165) of patients with 1 degrees -HPT had whole PTH(1-84) values above the normal cut-off. The percentage of biologically active whole PTH(1-84) (pB%) in the pool of total immunoreactive "intact" PTH is higher in the normal population (median: 67.3%; SD: 15.8%; n = 56) than in uremic patients (median:53.8%; SD: 15.5%; n = 318; p < 0.001), although the whole PTH(1-84) values from uremic patients displayed a more significant heterogeneous distribution when compared with that of 1 degrees -HPT patients and normals. Moreover, the pB% displayed a nearly Gaussian distribution pattern from 20% to over 90% in patients with either 1 degrees-HPT or uremia. The specificity of this newly developed whole PTH(1-84) IRMA is the assurance, for the first time, of being able to measure only the biologically active whole PTH(1-84) without cross-reaction to the high concentrations of the aminoterminally truncated PTH fragments found in both normal subjects and patients. Because of the significant variations of pB% in patients, it is necessary to use the whole PTH assay to determine biologically active PTH levels clinically and, thus, to avoid overestimating the concentration of the true biologically active hormone. This new assay could provide a more meaningful standardization of future PTH measurements with improved accuracy in the clinical assessment of parathyroid function.

Adult↗
PubMed1996

Growth hormone-releasing hormone immunoreactivity in the brain, pituitary, and pineal of the goldfish, Carassius auratus.

Using antisera directed against carp growth hormone-releasing hormone (cGHRH), the distribution of immunoreactive (ir) structures in the brain, pituitary, and pineal of the goldfish, Carassius auratus, was investigated. The antisera were produced in rabbits by administration of cGHRH(1-44)-NH(2) followed by cGHRH(1-45)-OH for different periods of time, both coupled to human alpha-globulins via bisdiazotized benzidine as immunogen. Immunoreactive perikarya were visualized in the nucleus preopticus periventricularis (NPP), nucleus lateralis tuberis (NLT), nucleus of the lateral lemniscus (NLL), and the pineal. The preoptic area and area ventralis telencephali seemed to receive innervation from the NPP cells. The fibers of the NLT cell bodies extended caudally along the infundibular floor and innervated the hypophysis. The processes of the NLL perikarya extended rostrally along the lateral lemniscus, curved ventrally along the outer margin of the glomerular nuclear complex, and extended to the dorsal area of the lateral recess, while issuing fascicles that branched off and fanned out to innervate extensively the nucleus diffusus of the hypothalamic inferior lobes and the nucleus lateralis tori; several fiber bundles branched off and extended toward the lateral areas of the paraventricular organ area and even into the rostral region of the hypothalamic inferior lobes. A small percentage of the corticotrophs of the rostral pars distalis, a few cells in the central area of the pars intermedia (PI), and neurohypophysial fibers in the peripheral area of the PI of the pituitary gland had colocalization of GHRH-ir; GHRH-ir fibers in the rostral and proximal pars distalis were sparse. It is suggested that the GHRH-ir perikarya of the diencephalon may stimulate growth hormone release either by modulating the activity of somatotrophs or of other hypothalamic neurosecretory neurons. The innervation of the hypothalamic inferior lobe by GHRH-ir perikarya of the NLL suggests some influence on feeding processes. Presence of GHRH in the pineal implies involvement in some daily cyclic activity.

Animals↗
PubMed1993

Comparison of rectal and axillary temperatures in neonates admitted to a neonatal unit.

To determine whether axillary temperature could be used as an alternative to rectal temperature, 45 neonates were subjected to simultaneous rectal temperature and axillary temperature recordings. Each baby had an average of 10 recordings over the first 72 hours. The mean of these readings was taken and subjected to regression analysis and the 't' paired test. The overall mean difference between the rectal and axillary temperature was 0.3 degrees F. The mean difference was least in preterm small for date (SFD) babies (0.2 degrees F) and most in preterm appropriate for date (AFD) babies (0.5 degrees F). The mean difference was less in incubator babies (0.2 degrees F) compared to those in warmer cradles (0.4 degrees F). The regression analysis showed that the correlation was good in all groups except the term small for date babies. We conclude that axillary temperature could be used as an alternative to rectal temperature in neonates.

Axilla↗
PubMed1988

Doctors dilemma?

Explore the source record for details and available documents.

Adult↗
PubMed1980

Hyperparathyroidism following head and neck irradiation.

A history of head and neck irradiation in childhood or adolescence was found in 22 of 130 patients with primary hyperparathyroidism compared with only 12 of 400 control patients, a significant difference (P less than .025). Among 200 patients with a known history of childhood irradiation, biochemical or surgical evidence of hyperparathyroidism was found in ten, a prevalence of 5%. This is at least 30 times the prevalence of hyperparathyroidism in the general population (P less than .025). The data indicate that head and neck irradiation should be regarded as an important risk factor in the subsequent development of hyperparathyroidism.

Adenoma↗