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Biomedical subjects

S Cinti

Publications and source records attributed to S Cinti.

At least 127 records · Page 7Linked to original sources
PubMed1982

[Ultrastructural histochemical study of the mammary gland of the lactating rat].

The authors report the results of their ultrastructural study on the lactating mammary gland of the rat using Grimelius' silver stain, modified for electron microscopy. The granules of the gland proteic ésocrine secretion are positive to silver impregnation, both within the cytoplasm and in the alveolar lumen. These results are interesting since they suggest that Grimelius' stain positive structures, in the mammary gland, should not be necessarily interpreted as endocrine, at least in the rat.

Animals↗
PubMed1982

[Ultrastructural changes in the mammary gland of the lactating rat induced by treatment with bromocriptine].

The authors report the results of their ultrastructural study on the lactating mammary gland of the rat treated with bromocriptine. On the sixth day of treatment the mammary gland shows the same morphological features of the physiologically involuting mammary gland and the same morphological features of the mammary gland of the rat whose pups have been taken away. These results suggest that the pharmacologic treatment with bromocriptine induces a physiological involution of the lactating mammary gland in the rat and that the same drug abates the effect of the persisting suckling stimulus.

Animals↗
PubMed1982

Human malignant diffuse mesothelioma: submicroscopic study on the epithelial component.

A submicroscopic analysis of eight cases of malignant diffuse mesothelioma was performed. Unlike normal and hyperplastic mesothelium, well differentiated neoplastic cells were found to have a prominent RER and a conspicuous Golgi system. By using a postfixation in osmium ferrocyanide, an amorphous substance was observed in the extracellular space. These features may be related to the ability of malignant diffuse mesothelioma to produce large amounts of hyaluronic acid. The significance of groups of membrane bound dense bodies found in seven of eight cases examined is also discussed.

Cell Membrane↗
PubMed1980

Gangliocytic paraganglioma of the duodenum: case report.

A case of gangliocytic paraganglioma of the duodenum, the 25th in world medical literature, is reported. The histologic appearance consists of epithelioid cell nests resembling "Zellballen" of carotid body tumors, together with gangliocyte-like elements. Ultramicroscopically, the cytoplasm of the cells contains round electron-dense granules or a large number of filaments. Occasional cells contain both granules and filaments.

Adult↗
PubMed1979

[Particular ultrastructural aspects of human Paneth's cells].

Our research is about the ultrastructural aspects of Paneth cells obtained from human jejunal mucosa. The cells appear columnar in shape and show a roundish nucleus; their cytoplasm is characterized by a great amount of R.E.R. and by typical large granules in sopranuclear position. Our results confirm those previously described by other authors about the morphology of Paneth cells. In our specimens we have found moreover a variable number of particular cytoplasmic structures; these may show a fibrillar or granular matrix and are always surrounded by a membrane.

Cell Nucleus↗
PubMed1978

Failure of somatostatin to diagnose organic hyperinsulinism.

In four patients with organic hyperinsulinism (two with surgically proven beta-cell adenomas of the body of the pancreas) a standard tolbutamide test during continuous somatostatin infusion (5 microgram/min) was carried out. Tolbutamide induced insulin release was completely inhibited by somatostatin as in normal subjects. These results suggest that the inhibition test with somatostatin does not seem to be a better or safer way of diagnosing insulin producing tumours.

Adult↗
PubMed1976

[Serum calcium evaluation and incidence of primary hyperparathyroidism in hospitalized patients (author's transl)].

Automated laboratory procedures have made possible to "screen" a large population for specific biochemical abnormalities. Primitive hyperparathyroidism is for several respects an excellent disease model for testing "mass screening". Il is often asymptomatic, not uncommon, and is manifested by abnormalities in the levels of serum calcium and inorganic phosphorus, that can be detected cheaply with automated equipment. A computer program has been developed to screen patients with hypercalcaemia. During a period of 18 months 22720 hospitalized patients were investigated by the evaluation of serum calcium, and 80 hypercalcaemic patients were found. The diagnosis of primary hyperparathyroidism was established in 24 patients (in 19 histologically confirmed) so that the incidence of primary hyperparathyroidism (1,05%) compares favorably with that reported from some foreign Authors.

Adult↗
PubMedDate not supplied

Ultrastructural findings in the jejunal mucosa of children with IgA deficiency.

The primary aim of this work was to investigate the ultrastructural morphology of the small intestinal mucosa in children affected by selective IgA deficiency (SIgAD). Absorptive tests and intestinal biopsies were performed in a group of nine children with SIgAD, none of them presenting with diarrhea. Biopsy specimens were processed for routine histology and scanning or transmission electron microscopy (SEM and TEM). By light microscopy, the jejunal mucosa appeared normal in all but one patient with patchy villous atrophy. By SEM, two further biopsies showed small areas of flat mucosa surrounded by villous ridges of nearly normal height. By TEM, four out of the nine mucosal specimens showed degenerative changes of the surface epithelium, including enlargement of the mitochondria, apical accumulation of primary and secondary lysosomes, and shortening and branching of the microvilli. These findings suggest that the small intestinal mucosa of children with SIgAD often shows pathological changes, some of which are recognizable only at the ultrastructural level. The study of biopsy specimens by SEM seems to be a useful diagnostic tool when patchy mucosal damage may occur, as in some of our patients with SIgAD.

Child↗
PubMedDate not supplied

Unresponsive enteropathy associated with circulating enterocyte autoantibodies in a boy with common variable hypogammaglobulinemia and type I diabetes.

A 13-year-old boy with common variable hypogammaglobulinemia and type I diabetes developed a severe enteropathy that proved to be unresponsive to any treatment, including total parenteral nutrition. No evidence of known etiologies of malabsorption and/or secretory diarrhea was found in this subject. A high titer of complement-fixing enterocyte autoantibody was persistently found in the patient's serum. These features suggest that the enteropathy of this primarily immunodeficient subject had an autoimmune origin. A cycle of cyclophosphamide failed to show any amelioration of the diarrhea or a significant decrease in the autoantibody titer.

Adolescent↗
PubMedDate not supplied

Hairy cell leukemia: an ultrastructural study of hairy cells before and after interferon therapy.

AIMS AND BACKGROUND: To test the diagnostic relevance of the presence of ribosome-lamellae complexes (RLC) in 18 hairy cell leukemia (HCL) cases, and to correlate clinical response to interferon (IFN) therapy with hairy cell ultrastructural modifications in 5 of these cases. METHODS: Peripheral blood samples of 18 HCL patients were studied by transmission electron microscopy. Five of these patients received IFN treatment and subsequently were evaluated at different intervals for ultrastructural modifications of the peripheral blood. RESULTS: RLC were observed in 66.66% of our 18 HCL patients, but in less than 1% of all the cases contained in the files (consisting of over 8,000 cases) of our Electron Microscopy Unit. The microvilli disappeared after IFN therapy in the patients who did not display RLC before therapy (2 cases), whereas they were fewer, shortened and blunted, but still evident, in the cases where RLC had been observed before therapy (3 cases). Moreover, in the HCL cases with pretherapy RLC, neoplastic cells still synthesized RLC after IFN treatment, but their morphologic aspect was immature. CONCLUSIONS: Our study suggests that: 1) the presence of RLC, when associated to the hairy aspect of the cells, has considerable diagnostic value even though RLC are observed in other rare neoplastic and non-neoplastic conditions; 2) HCL cases with pre-therapy RLC exhibited a morphologic response to IFN therapy different from that of cases without pre-therapy RLC; 3) the quantitative and qualitative modifications of RLC following IFN treatment, as yet unexplained, are probably related to IFN action, in line with a previous report.

Adult↗
PubMedDate not supplied

RER-associated structure in parathyroid glands removed because of tertiary hyperparathyroidism.

We have performed a submicroscopic analysis of the parathyroid glands removed from 2 patients with tertiary hyperparathyroidism. In both cases an RER-associated structure was found, which has never been described in normal or pathologic conditions in protein-secreting cells. This finding was present in only one of the glands of each patient. Their morphologic features are described. The significance of this structure is also discussed.

Adult↗
PubMedDate not supplied

Ultrastructural features of cultured mature adipocyte precursors from adipose tissue in multiple symmetric lipomatosis.

The pathogenesis of multiple symmetric lipomatosis (MSL) is as yet unknown; however, some studies seem to indicate the neoplastic nature of this lesion. In this study we have examined the ultrastructural features of the adipocyte precursors of patients with MSL. The cells were isolated by collagenase, cultured, and then examined at the moment of their confluence in a monolayer. Their ultrastructural features were compared with those of cells of the abdominal subcutaneous adipose tissue taken from patients with nonsystemic, surgically treated illnesses. When examined immediately after isolation, the cells from normal tissue did not show significant differences from MSL cells. In culture, however, MSL grew more quickly into a monolayer and showed numerous nuclear pockets and cytoplasmic microfilaments, which were not seen in cells from normal tissue. These differences appear compatible with the neoplastic nature of the MSL adipocyte precursors.

Adipose Tissue↗
PubMedDate not supplied

Ultrastructural morphology of folliculo-stellate cells in human pituitary adenomas.

Fifty-six pituitary adenomas were studied by electron microscopy in a search for the presence of folliculo-stellate cells (FSCs) with the aim of evaluating their prevalence and ultrastructural morphology. FSCs were scattered in two adenomas (one oncocytoma and one densely granulated GH cell adenoma) and were numerous in a sparsely granulated GH cell adenoma; their overall prevalence was 5.4%. Ultrastructural examination of the three neoplasms revealed that FSCs were hypertrophic element with abundant cytoplasm and organelles (in contrast to FSCs of the normal pituitary) and no obvious signs of neoplastic transformation. Junctional complexes between FSCs were similar to those described in the normal gland. Numerous follicular structures were lined by FSCs. FSCs in pituitary adenomas are probably nonneoplastic, reactive cells showing signs of hyperactivity, similar to FSCs found during pituitary hypersecretion and in estrogen-induced tumor.

Adenoma↗
PubMedDate not supplied

Adipose tissues and obesity.

In rats and mice the adipose organ consists of different depots, the most important are: the dermic, subcutaneous and visceral depots. Some areas of these depots are brown in colour and correspond to brown adipose tissue, while most are white and correspond to white adipose tissue. Varying amounts of brown adipocytes are found in white areas. Brown adipocyte precursors are different from those of white adipocytes, but among the brown adipocytes found in white depots precursors with an intermediate morphology can also be found. Together with a rich vascular supply, brown areas receive abundant noradrenergic and sensory innervation. The gross anatomy and histology of the organ vary considerably in different physiological (cold acclimation, warm acclimation, fasting, lactation) and pathological (obesity) conditions, and also many important genes such as leptin and uncoupling protein 1 are expressed differently in the two cell types. These basic mechanisms should be taken into account when addressing the physiopathology of obesity and its treatment.

Acclimatization↗