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Biomedical subjects

S Chaudhuri

Publications and source records attributed to S Chaudhuri.

At least 73 records · Page 4Linked to original sources
PubMed1994

A case-control study of reproductive risk factors associated with cervical cancer.

Using the logistic-regression technique, a hospital-based case-control study of 177 married women with invasive squamous-cell cervical cancer and 149 hospital-visiting controls enabled evaluation of selected reproductive factors as risks. Early age at marriage was found to be the single best predictor of the disease status. However, those who married late but gave birth to a large number of children were generally found to be suffering from cervical cancer. The results support the hypothesis that it is not so much parity per se that enhances the risk, but the rapidity of multiple pregnancies that matters. Logistic analysis also revealed the independent influence of birth interval on the risk of cervical cancer. These findings warrant serious consideration in future studies, given the obvious implications for prevention. Other implications for the prevention of cervical cancer are briefly discussed.

Adult↗
PubMed1994

Allele-specific parental imprinting of dzr1, a posttranscriptional regulator of zein accumulation.

Parental imprinting describes the phenomenon of unequivalent gene function based on transmission from the female or male parent. We have discovered parental imprinting of an allele of the dzr1 locus that posttranscriptionally regulates the accumulation of 10-kDa zein in the maize endosperm. The imprinted allele of MO17 inbred origin, dzr1 + MO17, conditions low accumulation of the 10-kDa zein and is dominant when transmitted through the female but recessive when transmitted through the male. Analyzing endosperms with equal parental contributions of dzr1 + MO17 ruled out the possibility that the unequivalent phenotype of dzr1 + MO17 was due to parental dosage imbalance in the triploid endosperm. Second-generation studies show that the dominant or recessive phenotype of dzr1 + MO17 is determined at every generation based on immediate parental origin with no grandparental effect.

Alleles↗
PubMed1994

Cloning of a pig glucose transporter 4 cDNA fragment: use in developing a sensitive ribonuclease protection assay for quantifying low-abundance glucose transporter 4 mRNA in porcine adipose tissue.

A 246-bp fragment of porcine glucose transporter 4 (GLUT4) cDNA was cloned by polymerase chain reaction (PCR) from porcine adipose tissue RNA. Nucleotide sequences 1-138 and 139-246 of the GLUT4 cDNA share 78% sequence identity with exon 4a and 91% sequence identity with exon 4b of the human GLUT4 gene, respectively. The GLUT4 cDNA fragment was subcloned into pGEM-4Z vector to synthesize a highly specific riboprobe that hybridized only to human GLUT4 cDNA but not to human glucose transporter 1 (GLUT1) cDNA. Northern blot analysis of total RNA revealed the presence of a single transcript of 2.8 kb in porcine adipose tissue. Cloning a fragment of the GLUT4 cDNA enabled us to develop a ribonuclease protection assay for detecting porcine GLUT4 mRNA. The ribonuclease (RNase) protection assay is highly reproducible and retains a sensitivity level to as little as 2 pg of GLUT4 mRNA. The standard curve was linear between 2 and 128 pg of sense-strand GLUT4 RNA (r = .994). The ability to detect small quantities of GLUT4 mRNA is important when the abundance of GLUT4 mRNA is low and the quantity of tissue is limiting (e.g., when RNA is extracted from cultured adipose tissue). When porcine adipose tissue explants were cultured in the presence of insulin (10 ng/mL), GLUT4 mRNA abundance was increased. Development of a sensitive assay to quantify GLUT4 mRNA in porcine adipose tissue will enable us to conduct studies to increase our understanding of the molecular mechanisms by which porcine somatotropin (pST) regulates GLUT4 gene expression.

Adipose Tissue↗
PubMed1994

Delirium after cataract extraction: a prospective study.

A prospective study of 350 consecutive in-patients undergoing cataract extraction revealed the incidence of postcataract extraction delirium to be 1.7%. While in one case the delirium was due to anticholinergic toxicity, in the remaining 5 cases no organic cause was evident. Sensory deprivation was present in 3 patients. In addition to organic causes sensory deprivation was found to be an important cause of postcataract extraction delirium.

Adolescent↗
PubMed1993

The stimulation by dendritic cells of host-versus-graft reactivity in vivo.

Dendritic cells (DC) isolated from peripheral intestinal lymph of inbred Wistar rats were injected into the hind footpads of Hooded CBH/Cbi rats. Administration of 10(4) and 5 x 10(5) allogeneic DC produced approximately six-fold increase in popliteal node weight. This is only marginally greater than that caused by similar doses of syngeneic DC and about 30% greater than that caused by allogeneic mononuclear cells. The data indicated that allogeneic DC were not effective stimulators of host-versus-graft reactions in this assay.

Animals↗
PubMed1993

Mechanisms by which somatotropin decreases adipose tissue growth.

When growing pigs are treated daily with recombinantly derived porcine somatotropin (pST) for 30-60 d there is a dose-dependent decrease in lipid accretion. Maximal doses of pST can reduce lipid accretion by as much as 70%. The reduction in lipid accretion occurs because of a marked decrease in glucose transport and lipogenesis that is the result of a pST-dependent decrease in the ability of insulin to stimulate these processes in the adipocyte; lipolysis is not affected. The decrease in insulin sensitivity is not due to a decrease in insulin binding or insulin receptor kinase activity. Little is understood about the somatotropin (ST) intracellular signal pathway(s) that mediate the biological effects of ST. These effects are chronic rather than acute as was previously believed. This pattern likely reflects that ST decreases transcription of important insulin-responsive genes such as the muscle-adipose tissue transporter gene (GLUT4) and key lipogenic enzymes.

Adipose Tissue↗
PubMed1993

Immunomodulation and 'Ia' variability in dendritic lymph cells: a radiobinding immunoassay versus immune response study.

A radiobinding immunoassay technique has been employed to determine the 'Ia' antigens localised on the surface of the following immunocompetent cells: Dendritic lymph cells from peripheral intestinal lymph, peritoneal macrophages and thymocytes all gathered from sheep red blood cell treated animals, and the irradiated cell controls. A corresponding popliteal lymph node assay was performed following the administration of the same cells into the foot pad of allogeneic rats to determine the comparative immunogenic responsiveness. Dendritic lymph cells in free flowing lymph of sensitised, irradiated and control animals showed a significantly higher 'Ia' content and consequently greater immunogenicity as measured by the popliteal lymph node assay. Sheep red blood administration in animals may stimulate surface 'Ia' expression in lymph derived dendritic cells resulting in higher immune-responsiveness.

Animals↗
PubMed1992

Reconstitution of the activities of the RecBCD holoenzyme of Escherichia coli from the purified subunits.

The Escherichia coli RecBCD holoenzyme and the individual constituent subunits have been purified from overproducing strains. The purified RecBCD holoenzyme has a native molecular mass of approximately 330 kDa, indicative of a heterotrimer subunit assembly. The RecB, RecC, and RecD subunits can associate in vitro to give nuclease, helicase, ATPase, and Chi-specific endonuclease activities which are indistinguishable from those of the RecBCD holoenzyme. At concentrations at which the reconstituted RecB + C + D enzyme is very active, none of the individual RecB, RecC, or RecD subunits have readily detectable activities of the holoenzyme, except RecB protein which had previously been shown to exhibit DNA-dependent ATPase activity (Hickson, I. D., Robson, C. N., Atkinson, K. E., Hutton, L., and Emmerson, P. T. (1985) J. Biol. Chem. 260, 1224-1229). At higher concentrations and with shorter DNA substrates reconstituted RecBC protein exhibits low levels of helicase and exonuclease activity.

Adenosine Triphosphate↗
PubMed1992

Theoretical analysis of gel electrophoretic data for interaction of lysine rich histone with supercoiled DNA.

We demonstrate the possibility of using gel electrophoresis as a technique for the quantitative analysis of interaction of lysine rich histone with DNA. On the basis of theoretical framework for extended ligand binding to one-dimensional lattices such as DNA we have set up systems of equations which relate the ligand-to-DNA ratio to the observed gel migration distance of the complex. From the analysis of experimental data for gel electrophoresis of supercoiled DNA in the presence of lysine rich histones we have found that the observed variation of electrophoretic mobilities of the histone-DNA complexes at low histone-to-DNA ratios can be described by a non-cooperative binding behaviour. At this limit we have estimated the intrinsic binding constant to be of the order of 10(3) M-1.

DNA, Superhelical↗
PubMed1992

Growth hormone binding proteins in pig adipose tissue: number, size and effects of pGH treatment on pGH and bGH binding.

The present study was undertaken to determine the number and size of growth hormone binding proteins present in pig adipose tissue, determine if there were differences in binding of pGH and bGH to adipose tissue membranes and establish the effects of pGH treatment on GH binding. Administration of pGH (0, 25, 50 or 100 micrograms pGH/kg BW/d) for 7 d did not affect binding of [125I]bGH to adipose tissue microsomes. Maximum binding of bGH was approximately 8-fold higher than that observed for pGH. Half-maximal inhibition of [125I]bGH binding was observed at 11 ng/ml of bGH. In contrast, a more than 10-fold greater concentration of pGH was required to half-maximally inhibit [125I]pGH binding. bGH and pGH both bound to the same GH binding proteins (Mr of 92,000, 73,000 and 53,000). The GH binding proteins appear to be produced by post-translational modification of a single GH receptor transcript rather than alternative splicing of a primary transcript since only one GH receptor mRNA transcript (4.2 kb) was detected on Northern analysis. Our findings indicate that: 1) bGH is the preferred ligand to use to study GH binding in pig adipose tissue membranes (or adipocytes); 2) exogenous pGH does not alter GH binding; and 3) only one GH receptor mRNA transcript is present in pig adipose tissue.

Adipose Tissue↗