Biomedical subjects

S Cai

Publications and source records attributed to S Cai.

At least 109 records · Page 6Linked to original sources

PubMed1999

[The association of radiation-induced apoptosis with radiosensitivity of cervical carcinoma].

OBJECTIVE: To study the relationship between apoptosis, radiation-induced apoptosis and radiosensitivity of cervical carcinoma and find new markers for predicting radiosensitivity. METHODS: TdT-mediated bio-dUTP nick end labeling (TUNEL) method was used to check apoptosis cells. The association of radiation-induced apoptosis with tumor volume shrinkage, radiation damage grade and local control rate was analysed. RESULTS: There was no relationship between apoptosis before radiation and tumor volume shrinkage(P > 0.05); Radiation-induced apoptosis had a significant association with tumor volume shrinkage (P < 0.01); In patients with radiation-induced apoptosis 10 or more, the grade of radiation damage IIb-III accounted for 76% (13/17), while for less than 10, it was 39% (5/13, P < 0.05); After 6 months of radiotherapy, local control rate was 88% (15/17) in the former, 69% (9/13) in the latter, respectively (P > 0.05). CONCLUSION: Radiation-induced apoptosis has some reference value for predicting radiosensitivity of cervical carcinoma.

Apoptosis↗

PubMed1999

Gene deletion and carrier detection in the family of Becker muscular dystrophy by short tandem repeat sequence polymorphism.

OBJECTIVE: To type haplotypes among the patients, carriers and normal offspring in a family of males with Becker muscular dystrophy in one generation by allelic fragment length polymorphism analysis. METHODS: Deletion analysis of the patients were performed using multiplex polymerase chain reaction (PCR) of amplification with 9 dystrophin exon primers. Intragenic short tandem repeat (STR) sequence (STR44, STR45, STR49 and STR50) were amplified by PCR to analyse allelic fragment length polymorphisms in the members of the family. RESULTS: The deletions of exons 17, 19 and 45, as well as deletions of allelic fragments at the loci of STR44 and STR45 were determined in the patients. Hemizygosity at those two loci were detected and carrier status ascertained in the mother of the patients. The normal haplotypes were typed in the sister of the patients. CONCLUSION: The method of STR sequence polymorphism analysis can determine haplotypes at normal status or at risk status. It would be used in prenatal diagnosis and carrier detection in the families of Duchenne and Becker muscular dystrophy.

Adolescent↗

PubMed1999

[Implementation of "four-early principle" to raise the successful rate in mass burn casualties].

OBJECTIVE: To evaluate the feasibility and result of "four-early principle" in the treatment of mass burn casualties. METHODS: During the last 12 years, a total of 155 burn patients were admitted in 18 groups, and the following principles were implemented; early rapid fluid resuscitation during shock stage for delayed resuscitation patients; early tracheostomy to assist air passage humidification and lavation for moderate and severe inhalation injury; planning escharectomy and grafting during shock stage in patients with extensive deep burns; emphasizing early stage enteral nutritional support. RESULTS: The successful rate of treatment was raised, and the incidence of complications decreased in mass casualties with serious burn after implementing early stage treatment principles. CONCLUSION: The application of "four-early principle" on the treatment of mass burn casualties is feasible and effective.

Adolescent↗

PubMed1999

Significant reduction of T-cell chemotaxis to MCP-1 in patients with primary and metastatic melanoma.

OBJECTIVE: To address whether circulating T-cells from the patients with primary and metastatic malignant melanoma show altered chemotaxis to monocyte-chemotactic protein 1 (MCP-1) and its relation to tumor infiltrating lymphocyte (TIL) and metastasis. METHODS: Chemotactic response of T-cells towards MCP-1 and immuno-histochemistry study of TIL and tumor-associated-macrophages (TAM) were investigated in patients with primary and metastatic melanoma compared to patients with basal cell carcinoma and healthy persons. RESULTS: T-cells from patients with primary and metastatic melanoma showed a significantly decreased chemotactic migration towards MCP-1 and T-cells from patients with basal cell carcinoma showed normal chemotactic response. Immuno-histochemistry study showed that there was no correlation between the number of TIL and the decreased chemotaxis of circulating T-cells to MCP-1 in patients with primary melanoma. CONCLUSIONS: Circulating T-cells from patients with primary and metastatic melanoma showed a MCP-1-specific decrease in chemotactic migration. This may be due to abnormal expression or modulation of MCP-1-receptor expression on these cells.

Adult↗

PubMed1999

[Expression of MAGE-1 gene in hepatocellular carcinoma].

OBJECTIVE: To investigate the expression of MAGE-1 mRNA in human hepatocellular carcinoma (HCC) and to discuss the possibility of using MAGE-1 antigens as a new peptide vaccine for immunotherapy for HCC. METHODS: The expression of MAGE-1 mRNA in HCC tissue and the adjacent non-HCC liver tissue was detected using RT-PCR in 45 samples of HCC, 16 samples of cirrhosis and 12 samples of normal liver tissue. The 6 samples of MAGE-1 mRNA positive PCR products were DNA sequenced. The sequenced fragments of MAGE-1 cDNA were used as template to which a [alpha 32P] labeled probe was synthesized and employed for Southern blot analysis. HLA class I-A and I-B typing of 43 HCC patients were assayed by ELISA test. RESULTS: Of the 45 HCC samples, 32 expressed MAGE-1 mRNA. None of the HCC adjacent tissue or liver tissue from cirrhosis or normal liver samples expressed MAGE-1 mRNA. The DNA sequence confirmed that the target gene fragment in all the 6 samples of PCR products was MAGE-1 cDNA. Point mutation occurred in 3 samples resulting in alternation of amino acid residues in 2 positions. Southern blot analysis showed that MAGE-1 was also detectable in HCC tissue which was MAGE-1 positive in RT-PCR assay, but not detectable in non-HCC liver tissue. In HCC patients, the predominant types of HLA were A2(53.5%), A11(25.6%), A24(20.9%), A33(20.9%), B13 (28.3%) and B35(23.2%). MAGE-1 mRNA expression in HCC showed no correlation with the level of alpha-FP in blood and the diameter of tumor. CONCLUSIONS: MAGE-1 mRNA is expressed at a high percentage of HCC samples. This tumor rejection antigen may have potential to be used as a new peptide vaccine for immunotherapy for HCC. There are point mutations of MAGE-1 gene sequence in some Chinese HCC patients.

Adult↗

PubMed1999

A feasible and effective technique for the detection of Schistosoma japonicum cercaria-infested water.

OBJECTIVE: To develop a new technique for detecting Schistosoma japonicum (S. japonicum) cercariae so as to predict the distribution of area with high transmission potential and to control acute infection of schistosoma. METHODS: A C-6 film was established and used to adhere the cercariae on water surface and compared with a few other conventional detecting techniques. RESULTS: The average rate of the cercaria-recovery was 40% in laboratory and field using C-6 film, which was 20 times higher than that of traditional nylon-tissue adsorbing and precipitating method. Either in some areas where oncomelania were present or in some places where the snail had been under control, the technique could detect the cercariae. CONCLUSION: The technique can be characterized by a feasible, economical, promptly accessible method, especially for field application.

Animals↗

PubMed1999

[Prognostic value of cell proliferation and apoptosis in uterine cervical cancer treated with radiation].

OBJECTIVE: To study the prognostic value of cell proliferation and apoptosis in cervical cancer patients treated with radiation. METHODS: A retrospective study was done in 40 radiotherapy-uncontrolled cases of squamous-cell carcinoma of the cervix uteri and an equal number of cases, matched in age, clinical stage, tumor size and histologic grade, which had been cured by radiotherapy. Cell proliferation was judged by the proliferating cell nuclear antigen (PCNA)-positively stained cell number x intensity of staining, and number of cells in mitosis per 10 high-power microscopic fields (HPF). Apoptotic cells were identified by TUNEL method and the result was expressed as the number of cells undergone apoptosis per 10 HPF. RESULTS: The average level of PCNA expression was 5.38 +/- 3.21 in the cured patients and 7.50 +/- 3.16 in the radiotherapy-uncontrolled patients (P < 0.01). The number of mitotic cells/10 HPF was 6.33 +/- 5.15 in the cured patients and 11.33 +/- 7.50 in the uncontrolled patients (P < 0.001). The number of apoptotic cells/10 HPF was 20.90 +/- 8.19 in the cured patients and 27.13 +/- 17.28 in the uncontrolled patients (P < 0.05). The ratio of number of apoptotic cells to that of mitotic cells (A/M) was 4.97 +/- 3.31 in the cured and 3.36 +/- 2.83 in the uncontrolled (P < 0.05). CONCLUSION: In cervical cancer, PCNA expression, the number of cells in mitosis and that of cells in apoptosis are significantly increased while the A/M ratio is significantly decreased in radiotherapy-uncontrolled patients compared to those of patients cured by radiotherapy. These parameters may be of value of predict response to radiotherapy and prognosis of patients with cervical cancer.

Apoptosis↗

PubMed1999

[Interaction of cell adhesion to materials in tissue engineering].

OBJECTIVE: To investigate the adhesive interactions of cells with materials and the effects of material properties on cell adhesion in tissue engineering. METHODS: By looking up the recent literatures dealt with adhesive interactions of cells with materials and reviewing previous work on the adhesion of tissue-derived cells to materials. RESULTS: The adhesion characteristics of cells to materials not only depend on the nature of materials, including bulk and surface properties, surface modification, surface morphology, net charge, porosity and degradation rate, but also on the expression of cell surface molecules and their interaction with the material. CONCLUSION: The quantitative measure and biophysical mechanisms of cell adhesion to materials might be very important in tissue engineering.

Biocompatible Materials↗

PubMed1999

[Preliminary evaluation of xenotransplantation and utilization of native pig breeds in China].

OBJECTIVE: To evaluate the general situation of the native pig breeds and the relative present study conditions for better development of xenotransplantation in China. METHODS: By comparing the profits between the pigs and the non-human primates as the potential donors for xenotransplantation and emphasizing the source of the pigs as the organ donors from the transplantation studies, we analyzed the possible values of the native pig breeds as the donors. RESULTS: As one of the richest resources in the world, the species variation and relative genetic stability of Chinese native pig breeds could be the very valuable resources for xenotransplantation study and utilization. As a reverse, the xenotransplantation could provide opportunity for more economically and environmentally utilization of the pigs besides as the meat supply. CONCLUSION: As a very valuable and potential resource of organ donor, the native pig breeds of China might be noticed by the xenotransplantation colleagues in the world. It is necessary, to keep the balance among the risk vs. benefit and the protection vs. utilization of this valuable resource.

Animals↗

PubMed1999

[Studies on identification of yinchaihu by UV and IR].

OBJECTIVE: To study the identification of nine species of Yinchaihu derived from four genera of Caryophyllaceae, namely, Stellaria dichotoma var. lanceolata, St. dichotoma var. dichotoma, Arenaria juncea, A. capillaris var. glandulifera, Silene fortunei, Si. jenisseensis, Gypsophila pacifica, G. oldhamiana and G. licentiana. METHOD: UV and IR of alcohol extraction. RESULTS: The UV and IR spectra of these 9 plant roots have some difference. CONCLUSION: St. dichotoma var. lanceolata specified in the Chinese Pharmacopoeia can be distinguished from the other plants and all these 4 genera can be differentiated from one another by both UV and first derivative spectra. The genus Stellaria can be identified from the other 3 genera by IR.

Caryophyllaceae↗

PubMed1999

[Effect of po li gan capsule on immunofunction in mice].

This study aimed at the immune response of mice to Po Li Gan Capsule (PLGC). The effects of PLGC on phagocytosis of phagocyte, on serum hemolysin, and on IL-1, IL-2 in mice were investigated. The results showed that the coefficient and index of phagocytosis obviously increased in the experiment group, compared with that in the control group (P < 0.01); the serum hemolysin in the experiment group increased too (P < 0.01). The results also showed that PLGC could restore the capacity of macrophage for secreting IL-1 that had been inhibited by cyclophosphamide and PLGC could enhance the release of IL-2 from spleen cell in the experiment group (P < 0.05-0.01). These suggest that PLGC enhances both cellular and humoral immunity function in mice.

Adjuvants, Immunologic↗

PubMed1999

[Isolation and identification of two flavonoids from Taraxacum mongolicum Hand.-Mazz].

OBJECTIVE: To study the chemical constituents of Taraxacum mongolicum. METHOD: Compounds were separated by column chromatography on silica gel and polyamide, and their structures were determined by spectral analysis and chemical evidence. RESULT: Two flavonoid compounds were separated and elucidated as isoquercitrin and hyperin. CONCLUSION: These two compounds were isolated from the plant for the first time.

Flavonoids↗

PubMed1999

[Rapid determination of cefazolin in porcine serum by high performance capillary electrophoresis].

A rapid high performance capillary electrophoretic method was established to assay cefazolin in porcine blood serum. Porcine serum was deproteined by 60% acetonitrile and superlants were injected with 10 s pressure load. The final assay condition was: 40 cm x 75 microns silica capillary, 50 mmol/L bicarbonate buffer at pH10, 20 kV for 5 min, 254 nm UV detection. The assay was linear within the range of 5-320 micrograms/ml, the intra-day and inter-day relative standard deviations ranged from 1.7% to 8.0%. Analytical recovery of cefazolin was 107.9%, the limit of detection was 1.5 micrograms/ml, the limit of quantitation was 3.6 micrograms/ml. To investigate the pharmacokinetic profile of cefazolin in pig, six adult pigs were injected with 16 mg/kg cefazolin and all data was analyzed by pharmacokinetic software 3P87. Cefazolin metabolism in adult pigs appeared to be a typical two-compartment model with a clearance of 8.43 ml/(kg.min).

Animals↗

PubMed1999

[Effects of yi qi tong mai oral liquid on acute myocardial ischemia in dogs].

The objective of this study was to assess the prophylactic and therapeutic effects of Yi Qi Tong Mai Oral Liquid (YQTMOL) on acute myocardial ischemia. The models of ischemia were made, in which the anterior coronary arteries of anesthetized dogs were ligated. Four groups of dogs were examined and compared. We determined the extent of their myocardial ischemia and infarction by means of epicardial mapping and N-BT staining. The results showed that in the treatment groups, the increase of sigma-ST was inhibited 30-180 minutes after the oral administration of YQTMOL(compared with control group, P < 0.01), and the sigma-ST was decreased (P < 0.05). Furthermore, in the group dosed with YQTMOL (10 g/kg), the ratios of "area of infarction/heart weight" and "area of infarction/ventricle weight" both decreased (compared with control group, P < 0.05). These findings indicated that YQTMOL reduced the area of myocardial ischemia and the degree of myocardial infarction in dogs, so it could be effective for the prevention and treatment of acute myocardial ischemia.

Animals↗

PubMed1999

[A study on adhesive properties of transformed human embryonic tendon cells to PLA and PLGA].

To study the adhesive properties of transformed human embryonic tendon cells (THETCs) to polylactic acid(PLA) and polylactic-co-glycolic acid 85/15 (PLGA 85/15), the adhesive mechanical properties of single THETCs to PLA films (average thickness 18.6 microns) and PLGA 85/15 films (average thickness 17.3 microns) were measured by means of micropipet aspirating technique. The rsults showed that the attachment rate and adhesive force for two polymer groups(PLA precoated with bovine serum albumin (BSA) and PLGA 85/15 precoated with BSA) were significantly lower than those for two control groups(PLA and PLGA 85/15), respectively, while the attachment rate and adhesive force for other two polymer groups (PLA precoated with poly-D-lysine (PDL) and PLGA 85/15 precoated with PDL) were significantly higher than those for two control groups, respectively, and that the adhesive properties of THETCs to PLGA 85/15 films were better than those of THETCs to PLA films. These demonstrate that BSA can inhibit THETCs attachment to polymer, and PDL can promote THETCs attachment to polymer, and suggest that PLGA 85/15 films were better than those of THETCs to PLA films. These demonstrate that BSA can inhibit THETCs attachment to polymer, and PDL can promote THETCs attachment to polymer, and suggest that PLGA 85/15 coated with PDL may be used as a new type attachment substrate for THETCs.

Cell Adhesion↗

PubMed1999

[A new viscoelastic model for granulocytes].

In this paper, a new viscoelastic model for granulocytes was introduced. The stress was placed on the theory and its experimental results of three-parameter viscous model with micropipette aspirating technique. The small deformation of single rat granulocyte into the micropipette was analyzed by the model. Within the small deformation, the fit of the experiment data using the three-parameter viscous model was more satisfactory than that using the standard solid model, and the former suggested that cytoplasm of granulocyte was treated as a liquid rather than a solid.

Animals↗

PubMed1999

[The advances of research for problems of developing flow in blood vessel].

The development and the tendency of the problems of developing flow in the vessel are reviewed in this paper. It is pointed out that the problems of developing flow of viscoelastic blood vessel are front subjects, and that consideration shouldbe be give to three aspects, namely the blood flow, the blood vessel's wall motion and the coupling between the blood and the wall.

Blood Flow Velocity↗

PubMed1999

Feasibility study of an ultrasound contrast agent (levovist) in color Doppler imaging of liver neoplasms.

The purpose of this study was to determine the efficacy of using an ultrasound contrast agent (levovist) to enhance the color Doppler imaging of liver neoplasms. Thirty patients with hepatic tumors were enrolled in this study. After intravenous administration of levovist, the color Doppler signals of normal hepatic vessels were enhanced. In various hepatic tumors, the different patterns of tumor vascularity were observed, which had not been demonstrated in conventional non-contrast color Doppler imaging. In 11 of 16 patients with hepatocarcinoma, additional color Doppler signals were observed in the central part of the tumors. On the contrary, 3 patients with metastatic liver lesions the enhanced color Doppler signals appear only at the peripheral of tumors. A typical rim-like color enhancement was seen in 2 of the 3 cases. In six patients with hepatic hemangiomas contrast-enhanced color Doppler imaging demonstrated the blood vessels at the margin of the neoplasms. Contrast-enhanced color Doppler imaging improves the visualization of the hepatic neoplasm vascularity. This technique holds great promise for detecting small liver tumors and differentiating hepatic neoplasms.

Adult↗