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Biomedical subjects

S C Roy

Publications and source records attributed to S C Roy.

At least 19 recordsLinked to original sources
PubMed1997

Electrophoretic detection of myeloperoxidase, protease, lactoferrin and lysozyme in buffalo polymorphonuclear granular acid extracts.

Polymorphonuclear (PMN) cells of more than 90% viability and 92% purity were isolated from the peripheral blood of buffaloes. The cationic proteins were extracted with 0.2 mol/L sodium acetate, pH 4.0 from the granules in the PMN and subjected to both non-denaturing and denaturing acid urea polyacrylamide gel electrophoresis (AUPAGE) for identification of myeloperoxidase (MPO), lysozyme, protease activity and lactoferrin. Protease was identified using alpha-naphthyl acetate as substrate, while lactoferrin was identified using a reference lactoferrin from bovine milk in AUPAGE, and by double immunodiffusion and Western blot techniques. Based on AUPAGE, lysozyme was found to be most cationic of all the proteins and peptides from the PMN granules as was evident from reference lysozyme run. The results indicated that the granules in buffalo PMN cells have lysozyme, protease, MPO and lactoferrin.

Animals↗
PubMed1996

Effect of Pasteurella multocida vaccination on buffalo polymorphonuclear hydrogen peroxide and nitric oxide production.

An attempt was made to investigate the effect of Pasteurella multocida on certain microbicidal reactive oxygen and nitrogen intermediates released by the polymorphonuclear cells (PMNs) from the vaccinated animals. The PMNs from the peripheral blood of both control and experimental buffaloes vaccinated against haemorrhagic septicaemia were isolated. PMNs from control animals upon activation with P. multocida lipopolysaccharide (LPS) and live P. multocida cells generated higher levels of hydrogen peroxide (H2O2) and nitric oxide (NO-) than the non-activated cells (P < 0.01). In the presence of P. multocida LPS, PMNs from animals vaccinated against haemorrhagic septicaemia generated significantly higher H2O2 (P < 0.05) and NO- (P < 0.01) than the PMNs from control animals. L-Arginine when added to the activation medium enhanced the production of NO- in a dose-dependent manner. This indicated the role of arginine in NO- production. The study suggested that buffalo PMNs possessed a potent oxidant defence system even in the presence of P. multocida, an antiphagocytic bacterium.

Animals↗
PubMed1996

Immunogenicity of outer membrane protein of Pasteurella multocida in buffalo calves.

Outer membrane protein (OMP) from Pasteurella multocida serotype B:2 was extracted and characterized using SDS-PAGE. Ten major polypeptide bands of MW 88 to 25 kDa were observed. Immunoblotting suggested that the polypeptides of MW 44, 37 and 30 kDa were the major immunogens. Buffalo calves vaccinated with the OMP vaccine or a commercial haemorrhagic septicaemia oil adjuvant vaccine developed highest mean log10 ELISA titres day 21 postvaccination (pv). Antibody titres detectable in these animals using an indirect haemagglutination assay were lower than the ELISA titres but the pattern of the antibody response was similar. A passive mouse protection assay revealed that the maximum protection against the challenge infection was conferred by the serum collected from both the groups on day 21 pv and 26 pv. Following challenge with virulent P. multocida cells, all the five OMP vaccinated animals survived whereas only 2 out of the 3 HS oil adjuvant vaccinated animals withstood the challenge. Results suggested that OMP was protective and could be used in vaccines against haemorrhagic septicaemia.

Animals↗
PubMed1977

Orientation of interphase chromosomes as detected by Giemsa C-bands.

The orientation of Giemsa C-bands has been studied in mitotic and interphase cells of Allium cepa. A sativum and of Aloe vera. The C-bands in these three species are located at the telomeres, secondary constriction region of the nucleolar chromosomes and the centromeric regions, respectively. Observations in A. cepa and Aloe indicate clearly that the interphase chromosomes are non-random in their orientation and possibly maintain their telophase configuration through the attachment of telomeres and perhaps of kinetochores with the nuclear membrane. Electron micrographs of onion cells also reveal that certain heterochromatic segments are associated with the nuclear membrane.--The nucleolar interstitial C-bands in A. sativum remain free in the nucleoplasm and may come close to each other due to heterochromatic attraction. Such a heterochromatic attraction is also evident between telomeric regions and between centromeres. However, a two by two attachment could not be noticed. A diagrammatic representation of the orientation of interphase chromosomes has been presented.

Azure Stains↗
PubMed1977

Orientation of Giemsa C-bands in interphase cells of Allium cepa L.

Orientation of Giemsa C-bands in Allium cepa was studied in both mitotic and interphase cells. It has been shown that telophase orientation of the chromosome is maintained throughout the interphase and early phophase. It has been assumed that this non-random orientation is due to anchorage of the telomeres with the nuclear membrane. Contrary to earlier observations, 2 by 2 pairing of the telomers could not be traced to this species.

Chromosomes↗
PubMed1975

An inducible riboflavin synthetase from a pseudomonad.

Riboflavin synthetase activity is induced in a strain of Pseudomonas fluorescens-putida intermediate only under conditions permitting an accumulation of compound P, a 2,4-dioxopteridine, in the medium. The effect of different amino acids and sugars on the production of compound P and riboflavin synthetase was determined. The enzyme was partially destroyed by ammonium sulphate fractionation. It is inhibited by heavy metal ions and PCMB. PCMB inhibition can be almost completely reversed by GSH.

Amino Acids↗
PubMed1973

Protein synthesis in vitro in a system from plant mitochondria.

A mitochondrial system from 48h-germinating seeds of Vigna sinensis (Linn.) Savi is capable of incorporating l-[U-(14)C]valine into proteins and is practically insensitve to cycloheximide, but highly sensitive to chloramphenicol and fusidic acid, a potent inhibitor of peptide-chain elongation factor. A system consisting of mitochondrial S-100 fraction and ribosomes from the same source and other cofactors is capable of polyphenylalanine synthesis and behaves similarly with respect to these inhibitors.

Carbon Radioisotopes↗