Biomedical subjects

S C Pandey

Publications and source records attributed to S C Pandey.

58 records · Page 4Linked to original sources

PubMedDate not supplied

Beta-adrenergic receptor subtypes in stress-induced behavioral depression.

The purpose of this study was to examine the role of beta-adrenergic receptors in an animal model of stress-induced behavioral depression. beta-Adrenergic receptors in several brain regions and leukocytes of rats were determined by receptor binding techniques using 125I-cyanopindolol (cyp) as ligand and propranolol as displacer for total beta-adrenergic receptors, and ICI 86,406 for beta 1- and ICI 118,551 for beta 2-adrenergic receptors. We observed that the maximum number of binding sites (Bmax) and the apparent dissociation constant (Kd) of 125I-cyp binding to total beta-adrenergic receptors were increased in hippocampus of stressed rats with escape deficits (48 h after training) as compared to control rats. This increase was due to an increase in Bmax and Kd of 125I-cyp binding to beta 1-adrenergic receptors but not to beta 2-adrenergic receptors. There was no significant difference in beta 1-adrenergic receptors in cortex and cerebellum or beta 2-adrenergic receptors in hippocampus, cortex, cerebellum, or leukocytes of stressed (48 h after training) rats with escape deficits as compared to control rats. Interestingly, it was observed that beta 1- and beta 2-adrenergic receptors in various brain regions (cortex, cerebellum, and hippocampus) and beta 2-adrenergic receptors in leukocytes of stressed rats (10 days after training) were not significantly different from control rats, although escape deficits were still present. These results suggest that abnormalities in adrenergic neurotransmission are associated with an upregulation of beta 1-adrenergic receptors, which in turn may be involved in the early stages of behavioral deficits caused by uncontrollable shock.

Animals↗

PubMedDate not supplied

Chronic ethanol consumption decreases the phorbol ester binding to membranal but not cytosolic protein kinase C in rat brain.

We examined the effect of 60 days of ethanol treatment on protein kinase C (PKC) in membrane and cytosolic fractions of the rat cerebral cortex. Membranal and cytosolic PKC were determined by binding technique using [3H]-phorbol 12,13 dibutyrate (PDBU) as radioligand and phorbol 12-myristate 13-acetate (PMA) as displacer. Chronic ethanol consumption resulted in a decrease in the maximum number of binding sites (Bmax) of [3H]-PDBU binding to membranal PKC without significant change in the apparent dissociation constant (KD) in the rat cortex. We also observed that chronic ethanol consumption had no significant effect on Bmax or KD of [3H]-PDBU binding to cytosolic PKC in the rat cerebral cortex. These results suggest that chronic ethanol consumption leads to the down-regulation of brain PKC associated with membrane but not with cytosol.

Alcoholism↗

PubMedDate not supplied

Chronic ethanol effects on the expression of phospholipase C isozymes and Gq/11-protein in primary cultures of astrocytes.

The goal of this investigation was to determine whether chronic ethanol exposure alters the expression of specific protein sites distal to receptors [Gq/11-protein, phospholipase C (PLC) isozymes] in primary cultures of astrocytes obtained from neonatal rat cortex. The protein expression (immunolabeling) of the PLC-beta 1, -gamma 1, -delta 1 isozymes and of the Gq/11 alpha subunit was determined by Western blot analysis using specific monoclonal antibodies. The PLC-beta 1, -gamma 1, -delta 1 isozymes and the Gq/11 alpha subunit migrated at apparent molecular masses (PLC-beta 1, 41 kDa; PLC-gamma 1, 145 kDa. PLC-delta 1, 85 kDa: Gq/11 alpha protein, 42 kDa). Thus, a PLC-beta 1 fragment of 41 kDa, but not the biologically active 150 kDa PLC-beta 1, was detected in primary cultures of astrocytes. Chronic ethanol exposure (4 days) resulted in a significant increase in the expression of PLC-delta 1, whereas under identical conditions, the expression of PLC-beta 1, -gamma 1, and of the alpha subunit of Gq/11 protein was not significantly altered in astrocytes. These results suggest that chronic ethanol exposure results in an increased expression of the PLC-delta 1, isozyme in primary cultures of astrocytes.

Animals↗

PubMedDate not supplied

The status of oral hygiene in cleft lip, palate patients after surgical correction.

UNLABELLED: The cleft lip and palate patients usually present a number of problems viz. altered oral anatomy leading to changes in oral physiology diminishing the self-cleansing ability of individual. The handicapped children are unable to maintain their oral hygiene properly. The present study was formulated with the aim that does normalization of oral anatomy have its effect on improvement of oral hygiene? An assessment of oral hygiene index-simplified was performed between preoperative and postoperative values in the same patient at KGMU and KGDU. A total of 50 cases were recorded in two groups of 25 each: (i) 6 years. The observations are statistically analyzed by paired 't' test to get the significance of results. RESULTS: The data analyzed showed the significant decrease in oral hygiene indices observed in both groups. A relative significance in oral hygiene status following surgery was observed. Both groups expressed greater significance when compared pre and postoperatively which is indicative of considerable improvement of oral hygiene after surgical correction. The study concludes that oral hygiene improves more in older cleft lip-palate cases following reconstruction of palatal vault, premaxilla and anterior lip seal by secondary bone grafting method when compared with oral hygiene indices results in primary periosteoplasty cases. The surgical correction of cleft lip palate enhances self-cleaning ability and better compliance to maintain oral hygiene in children as the age advances.

Age Factors↗