A gas-liquid chromatographic procedure for determination of SCH 39304 at low nanogram concentrations in serum, cerebrospinal fluid, and urine is presented. The methodology combines a high selectivity and sensitivity nitrogen-specific detector, a gas chromatograph equipped with a capillary "megabore" column, and an internal standard that is very similar in chemical structure to the drug being assayed. This method is suitable for both pharmacokinetic studies as well as for monitoring drug levels in patients receiving SCH 39304 for antifungal treatment.

Antifungal Agents↗

PubMedDate not supplied

Determination of tricyclic antidepressants by high-performance liquid chromatography.

A method is described for the determination of amitriptyline, nortriptyline, imipramine and desipramine at low nanogram concentrations in serum. Separation and quantitation are performed by reversed-phase, isocratic, high-performance liquid chromatography utilizing ultraviolet detection at 210 nm. An internal standard, chloramitriptyline, is used as an aid to quantitation. Recovery of all four tricyclics is greater than 84%. The relative standard deviation for the analysis of amitriptyline, nortriptyline, imipramine and desipramine is less than 6% at a concentration of 100 ng/mL.

Antidepressive Agents, Tricyclic↗

PubMedDate not supplied

Determination of pentobarbital and certain other barbiturates by capillary gas-liquid chromatography.

A rapid capillary gas-liquid chromatographic method for the determination of barbiturates in plasma at concentrations of clinical importance is presented. This method employs a rapid and simple extraction procedure and direct flame ionization detection with no derivatization.

Barbiturates↗

PubMedDate not supplied

Determination of codeine and morphine in human plasma by high performance liquid chromatography with serial electrochemical detection.

A method is described for the determination of codeine and its metabolite, morphine, at low nanogram concentrations in plasma. Analysis is accomplished by high-performance liquid chromatography utilizing a cyanopropyl normal bonded phase (NBP) column in reversed-phase mode and two electrochemical detectors in series configuration. Two internal standards are utilized, ethyl morphine for codeine and nalorphine for morphine. Codeine and morphine concentration data are presented for several patients receiving codeine-containing medications. The lower limit of detectability was 2.00 +/- 0.39 ng per mL for codeine and 1.20 +/- 0.83 ng per mL for morphine. The patient sample mean within-run coefficients of variation for codeine and morphine (at 10 ng per mL) were less than 10 percent, n = 30. The between-run coefficient of variation for codeine was also less than 10 percent (over a range of two to 190 ng per mL, n = 61), and was approximately 15 percent for morphine (over a range of two to 40 ng per mL, n = 31).

Chromatography, High Pressure Liquid↗

PubMedDate not supplied

Xanthogranulomatous pyelonephritis: a pathological, clinical and aetiological analysis of 87 cases.

Analysis of 87 cases of xanthogranulomatous pyelonephritis ( XPN ) from 1958 to 1983 (14 males, 73 females, ages 13-85) revealed an incidence of 1.4 cases/100 000 population per annum which is apparently increasing. Clinical, radiological and pathological investigations demonstrated universal urinary obstruction (77.5 per cent calculi, 17.5 per cent pelviureteric junction obstruction) and pathogenic organisms such as E. coli or Proteus were cultured from the urine in 72 per cent of cases. Rare complications included sinuses or fistulae to bowel. We believe that the combination of urinary obstruction and infection by organisms of low virulence initiate XPN , and that associated lipid is derived from renal pelvic adipose tissue. Problems of differential diagnosis are discussed in relation to the use of immunocytochemistry and electron microscopy.

Adolescent↗